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Lung Tissue Lysate (Tumor)

WB Whole Tissue Lysate Tumor Lysate
Catalog No. ABIN5608714
  • Species of Lysate
    Human
    Application
    Western Blotting (WB)
    Specificity
    Lung Tissue
    Characteristics
    Lung tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1 % Triton X-100, 1 % sodium deoxycholic acid, 0.1 % sodium dodecylsulfate, 5 μ,g/mL of aprotinin, 5 μ,g/mL of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5 % glycerol, 1 % sodium dodecylsulfate, 0.01 % bromophenol blue) containing 5 % β,-mercaptoethanol.

    Source: Human Lung Tissue
    Age: 73
    Gender: M
    Race: Black
    Diagnosis: Tumor
    Cancer Type: Adenocarcinoma
    Cancer Type (Details): Moderately differentiated
    Lysate Type
    Tumor Lysate
    Lysate Fraction
    Whole Tissue Lysate
  • Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    2 mg/mL
    Buffer
    SDS sample buffer containing 5 % β,-mercaptoethanol.
    Handling Advice
    Repeated freezing and thawing is not recommended.
    Storage
    4 °C/-80 °C
    Storage Comment
    Store at 2-8˚,C for continuous use. For extended storage, freeze working aliquots at -70˚,C.
    Expiry Date
    6 months
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