anti-Horse (Equine) ATPase, H+ Transporting, Lysosomal 31kDa, V1 Subunit E2 antibody for Western Blotting

Recommended ATPase, H+ Transporting, Lysosomal 31kDa, V1 Subunit E2 Antibody (supplied by: Log in to see )

Antigen
ATPase, H+ Transporting, Lysosomal 31kDa, V1 Subunit E2 (ATP6V1E2) Antibodies
  • ATP6E1
  • ATP6EL2
  • ATP6V1EL2
  • VMA4
  • 4930500C14Rik
  • Atp6e1
  • E1
  • ATPase H+ transporting V1 subunit E2
  • ATPase, H+ transporting, lysosomal V1 subunit E2
  • Atp6v1e2
  • ATP6V1E2
Epitope
Middle Region
11
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
23
8
7
1
1
1
Host
Rabbit
20
4
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
Application
Western Blotting (WB)
17
10
3
1
Options
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Catalog No. ABIN2787752
$ 289.00
Plus shipping costs $45.00

General

Antigen ATPase, H+ Transporting, Lysosomal 31kDa, V1 Subunit E2 (ATP6V1E2) Antibodies
Epitope Middle Region
(11), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
(23), (8), (7), (1), (1), (1)
Host Rabbit
(20), (4)
Clonality
Polyclonal
Conjugate Un-conjugated
(1), (1), (1)
Application Western Blotting (WB)
(17), (10), (3), (1)
Pubmed 1 reference available
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Product details

Target Details Application Details Handling References Images
Blocking Peptide Blocking peptide for this product available: ABIN973636
Sequence LMSTMRNQAR LKVLRARNDL ISDLLSEAKL RLSRIVEDPE VYQGLLDKLV
Predicted Reactivity Cow: 79%, Dog: 79%, Guinea Pig: 86%, Horse: 79%, Human: 100%, Mouse: 79%, Rabbit: 79%, Rat: 86%, Sheep: 79%
Characteristics This is a rabbit polyclonal antibody against ATP6V1E2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human ATP6V1E2

Target Details

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Antigen
Alternative Name ATP6V1E2 (ATP6V1E2 Antibody Abstract)
Background ATP6V1E2 is a subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. This isoform is essential for energy coupling involved in acidification of acrosome.
Alias Symbols: ATP6E1, ATP6EL2, ATP6V1EL2, MGC9341, VMA4
Protein Interaction Partner: C9orf16, ATP6V1G1, UBC,
Protein Size: 226
Molecular Weight 26 kDa
Gene ID 90423
NCBI Accession NM_080653, NP_542384
UniProt Q96A05
Pathways Transition Metal Ion Homeostasis, Proton Transport

Application Details

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Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 226 AA

Restrictions For Research Use only

Handling

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Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

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Background publications

Hillier, Graves, Fulton, Fulton, Pepin, Minx, Wagner-McPherson, Layman, Wylie, Sekhon, Becker, Fewell, Delehaunty, Miner, Nash, Kremitzki, Oddy, Du, Sun, Bradshaw-Cordum, Ali, Carter, Cordes, Harris et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4. ..." in: Nature, Vol. 434, Issue 7034, pp. 724-31, 2005

Images

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Supplier Images
Western Blotting (WB) image for anti-ATPase, H+ Transporting, Lysosomal 31kDa, V1 Subunit E2 (ATP6V1E2) (Middle Region) antibody (ABIN2787752) WB Suggested Anti-ATP6V1E2 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:1562500 Pos...