anti-Horse (Equine) MIS12 antibody for Western Blotting

Recommended MIS12 Antibody (supplied by: Log in to see )

Antigen
MIS12, MIND Kinetochore Complex Component, Homolog (S. Pombe) (MIS12) Antibodies
  • 2510025F08Rik
  • KNTC2AP
  • MTW1
  • hMis12
  • BcDNA:RE19545
  • CG18156
  • Dmel\\CG18156
  • Mis-12
  • dmMis12
  • AU044651
  • Misc12
  • RP23-83I13.9
  • mtw1
  • kntc2ap
  • MGC80789
  • ARABIDOPSIS MINICHROMOSOME INSTABILITY 12 (MIS12)-LIKE
  • ATMIS12
  • MINICHROMOSOME INSTABILITY 12 (MIS12)-LIKE
  • MINICHROMOSOME INSTABILITY 12 HOMOLOGUE
  • MIS12 HOMOLOGUE
  • MOK9.12
  • MOK9_12
  • MIS12, kinetochore complex component
  • CG18156 gene product from transcript CG18156-RA
  • MIS12 kinetochore complex component
  • MIS12 kinetochore complex component L homeolog
  • MIS12, MIND kinetochore complex component, homolog (S. pombe)
  • minichromosome instability 12 (mis12)-like protein
  • kinetochore protein Mis12
  • MIS12
  • Mis12
  • mis12.L
  • mis12
Epitope
Middle Region
7
4
2
2
2
1
Reactivity
Cow (Bovine), Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
23
1
1
1
1
1
1
Host
Rabbit
15
8
Clonality
Polyclonal
Conjugate
This MIS12 antibody is un-conjugated
1
1
1
Application
Western Blotting (WB)
16
7
5
4
4
4
2
1
Options
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Available images

Catalog No. ABIN2787627
$ 289.00
Plus shipping costs $45.00

General

Antigen MIS12, MIND Kinetochore Complex Component, Homolog (S. Pombe) (MIS12) Antibodies
Epitope Middle Region
(7), (4), (2), (2), (2), (1)
Reactivity Cow (Bovine), Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(23), (1), (1), (1), (1), (1), (1)
Host Rabbit
(15), (8)
Clonality
Polyclonal
Conjugate This MIS12 antibody is un-conjugated
(1), (1), (1)
Application Western Blotting (WB)
(16), (7), (5), (4), (4), (4), (2), (1)
Pubmed 1 reference available
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Product Details anti-MIS12 Antibody

Target Details MIS12 Application Details Handling References for anti-MIS12 antibody (ABIN2787627) Images
Blocking Peptide Blocking peptide for this product available: ABIN980093
Sequence TLTFFDELHN VGRDHGTSDF RESLVSLVQN SRKLQNIRDN VEKESKRLKI
Predicted Reactivity Cow: 86%, Horse: 86%, Human: 100%, Mouse: 91%, Rabbit: 86%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against MIS12. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human MIS12
Plasmids, Primers & others

Target Details MIS12

Product Details anti-MIS12 Antibody Application Details Handling References for anti-MIS12 antibody (ABIN2787627) Images back to top
Antigen
Alternative Name MIS12 (MIS12 Antibody Abstract)
Background MIS12 is part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis.
Alias Symbols: 2510025F08Rik, KNTC2AP, MGC2488, MTW1, hMis12
Protein Interaction Partner: PMF1, ZWINT, SUGT1, CBX5, CASC5, NSL1, NDC80, NF1, BUB1, DSN1, BUB1B, HSP90AA1, SKA1, UBC, SPC24, NUF2, MIS12, SPC25, TF, PIGR, SERPINA1, HP, FGB, FGA, CENPC, APOA1, CBX3,
Protein Size: 205
Molecular Weight 23 kDa
Gene ID 79003
NCBI Accession NM_024039, NP_076944
UniProt Q9H081
Pathways M Phase

Application Details

Product Details anti-MIS12 Antibody Target Details MIS12 Handling References for anti-MIS12 antibody (ABIN2787627) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 205 AA

Restrictions For Research Use only

Handling

Product Details anti-MIS12 Antibody Target Details MIS12 Application Details References for anti-MIS12 antibody (ABIN2787627) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-MIS12 antibody (ABIN2787627)

Product Details anti-MIS12 Antibody Target Details MIS12 Application Details Handling Images back to top
Background publications

Kline, Cheeseman, Hori, Fukagawa, Desai: "The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation." in: The Journal of cell biology, Vol. 173, Issue 1, pp. 9-17, 2006

Images

Product Details anti-MIS12 Antibody Target Details MIS12 Application Details Handling References for anti-MIS12 antibody (ABIN2787627) back to top
Supplier Images
Western Blotting (WB) image for anti-MIS12, MIND Kinetochore Complex Component, Homolog (S. Pombe) (MIS12) (Middle Region) antibody (ABIN2787627) WB Suggested Anti-MIS12 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive ...
Western Blotting (WB) image for anti-MIS12, MIND Kinetochore Complex Component, Homolog (S. Pombe) (MIS12) (Middle Region) antibody (ABIN2787627) WB Suggested Anti-MIS12 Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.12500 Posit...