anti-Human Ninein (GSK3B Interacting Protein) antibody for Flow Cytometry

Recommended Ninein (GSK3B Interacting Protein) Antibody

Antigen
Ninein (GSK3B Interacting Protein) (NIN) Antibodies
  • si:ch211-282c13.6
  • ninein
  • 3110068G20Rik
  • AI385615
  • AU024711
  • mKIAA1565
  • SCKL7
  • ninein
  • ninein (GSK3B interacting protein)
  • ninein L homeolog
  • NIN
  • nin
  • nin.L
  • lpg0126
  • LPC_0146
  • lpa_00185
  • Nin
Reactivity
Human
16
11
8
Host
Rabbit
23
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
Application
Flow Cytometry (FACS)
19
13
4
4
3
3
Options

Available images

Catalog No. ABIN2666189
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
1 ABIN2666188 FACS Rabbit IgG Polyclonal 0

General

Antigen Ninein (GSK3B Interacting Protein) (NIN) Antibodies
Reactivity Human
(16), (11), (8)
Host Rabbit
(23)
Clonality (Clone)
Polyclonal   ( )
Conjugate Un-conjugated
(1), (1), (1)
Application Flow Cytometry (FACS)
(19), (13), (4), (4), (3), (3)
Pubmed 6 references available
Supplier

Product details

Target Details Application Details Handling References Images
Purification The antibody was purified by antigen-affinity chromatography.
Clone Poly6028
Isotype IgG

Target Details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name Ninein (NIN Antibody Abstract)
Background Ninein (also known asGSK3β-interacting protein and ninein centrosomal protein) is a chromosome segregation ATPase with 5 reported isoforms, (molecular weights ranging from 236 to 246 kD). Ninein is localized at the centrosome and is thought to position and anchor microtubules minus-ends. Ninein is important for centrosomal function in epithelial cells. This protein is modified by phosphorylation. Ninein has also been shown to interact with CEP250/c-Nap1, CEP110, γ-tubulin, centrin, GSK3β, AIK, and PKA. The Poly6028 antibody recognizes the N-terminal region of human ninein and has been shown to be useful for Western blotting.
Pathways Maintenance of Protein Location

Application Details

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Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product details Target Details Application Details References Images back to top
Format Liquid
Buffer phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Upon receipt, store frozen at -20°C.

References

Product details Target Details Application Details Handling Images back to top
Background publications

Jay, Hammer, Nestor-Kalinoski, Diakonova: "JAK2 tyrosine kinase phosphorylates and is negatively regulated by centrosomal protein Ninein." in: Molecular and cellular biology, Vol. 35, Issue 1, pp. 111-31, 2014

Ibi, Zou, Inoko, Shiromizu, Matsuyama, Hayashi, Enomoto, Mori, Hirotsune, Kiyono, Tsukita, Goto, Inagaki: "Trichoplein controls microtubule anchoring at the centrosome by binding to Odf2 and ninein." in: Journal of cell science, Vol. 124, Issue Pt 6, pp. 857-64, 2011

Hurd, Zhou, Jenkins, Liu, Swaroop, Khanna, Martens, Hildebrandt, Margolis: "The retinitis pigmentosa protein RP2 interacts with polycystin 2 and regulates cilia-mediated vertebrate development." in: Human molecular genetics, Vol. 19, Issue 22, pp. 4330-44, 2010

Chen, Howng, Cheng, Chou, Huang, Hong: "Molecular characterization of human ninein protein: two distinct subdomains required for centrosomal targeting and regulating signals in cell cycle." in: Biochemical and biophysical research communications, Vol. 308, Issue 4, pp. 975-83, 2003

Ou, Mack, Zhang, Rattner: "CEP110 and ninein are located in a specific domain of the centrosome associated with centrosome maturation." in: Journal of cell science, Vol. 115, Issue Pt 9, pp. 1825-35, 2002

Hong, Chen, Chang, Wang, Sy, Chou, Howng: "Cloning and characterization of a novel human ninein protein that interacts with the glycogen synthase kinase 3beta." in: Biochimica et biophysica acta, Vol. 1492, Issue 2-3, pp. 513-6, 2000

Images

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Supplier Images
Western Blotting (WB) image for anti-Ninein (GSK3B Interacting Protein) (NIN) antibody (ABIN2666189) anti-Ninein (GSK3B Interacting Protein) (NIN) antibody