anti-Human ARAF antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended ARAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) Antibodies
  • zgc:92074
  • wu:fk45h07
  • ARAF
  • a-raf
  • araf
  • araf1
  • pks2
  • rafa1
  • A-RAF
  • ARAF1
  • PKS2
  • RAFA1
  • 1200013E08Rik
  • A-Raf
  • AW495444
  • Araf1
  • A-Raf proto-oncogene, serine/threonine kinase
  • A-Raf proto-oncogene, serine/threonine kinase L homeolog
  • A-Raf proto-oncogene, serine/threonine kinase S homeolog
  • Araf proto-oncogene, serine/threonine kinase
  • araf
  • ARAF
  • araf.L
  • araf.S
  • Araf
Epitope
AA 371-554
23
20
16
15
14
12
10
9
8
5
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
145
82
75
6
6
6
3
2
2
2
1
1
Host
Rabbit
120
25
Clonality
Polyclonal
Conjugate
This ARAF antibody is un-conjugated
5
5
5
3
3
3
3
3
3
3
3
3
3
3
2
2
2
Application
Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
97
52
39
29
27
13
6
3
2
1
1
1
Options
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Catalog No. ABIN2799807
$ 431.20
Plus shipping costs $45.00

General

Antigen V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) Antibodies
Epitope AA 371-554
(23), (20), (16), (15), (14), (12), (10), (9), (8), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(145), (82), (75), (6), (6), (6), (3), (2), (2), (2), (1), (1)
Host Rabbit
(120), (25)
Clonality
Polyclonal
Conjugate This ARAF antibody is un-conjugated
(5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(97), (52), (39), (29), (27), (13), (6), (3), (2), (1), (1), (1)
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Product Details anti-ARAF Antibody

Target Details ARAF Application Details Handling Images
Immunogen Recombinant fragment corresponding to a region within amino acids 371-554 of A-RAF
Isotype IgG

Target Details ARAF

Product Details anti-ARAF Antibody Application Details Handling Images back to top
Antigen
Alternative Name A-RAF (ARAF Antibody Abstract)
Background Synonyms: A-RAF antibody, ARAF1 antibody, PKS2 antibody, RAFA1 antibody, ARAF antibody, proto-oncogene Pks antibody, serine/threonine-protein kinase A-Raf antibody, A-Raf proto-oncogene serine/threonine-protein kinase antibody, v-raf murine sarcoma 3611 viral oncogene homolog 1 antibody, Oncogene ARAF1 antibody, Ras-binding protein DA-Raf antibody, proto-oncogene A-Raf-1 antibody, proto-oncogene A-Raf antibody, v-raf murine sarcoma 3611 viral oncogene homolog antibody
Molecular Weight 68 kDa
UniProt P10398
Pathways MAPK Signaling, RTK Signaling, Hepatitis C

Application Details

Product Details anti-ARAF Antibody Target Details ARAF Handling Images back to top
Application Notes Tested ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer ( pH 6.0) or Tris-EDTA buffer ( pH 8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ARAF(107-10259) antibody at 1:500 dilution.Immunofluorescence analysis of paraformaldehyde-fixed A431, using ARAF(107-10259) antibody at 1:200 dilution.Sample (30 μg of whole cell lysate) A: Hela 7.5 % SDS PAGE 107-10259 diluted at 1:1000 Sample (30 μg of whole cell lysate) A:NIH-3T37.5 % SDS PAGE 107-10259 diluted at 1:1000
Comment

A-RAF antibody [C1C3], 0.85 mg/ml (100 μl)

Restrictions For Research Use only

Handling

Product Details anti-ARAF Antibody Target Details ARAF Application Details Images back to top
Format Liquid
Concentration 0.85 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.