anti-Human BCL2 antibody for Enzyme Immunoassay

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • Bcl-2
  • PPP1R50
  • bcl-2
  • AW986256
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • BCL-2
  • Bcl2
  • BCL2, apoptosis regulator
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2a
  • B-cell CLL/lymphoma 2 protein
  • BCL2
  • Bcl2
  • bcl2a
  • BCL-2
Epitope
BH3 Domain
164
77
67
64
44
31
25
25
24
21
18
15
15
15
13
13
13
10
9
9
6
6
6
5
5
5
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1053
258
213
54
28
22
18
18
5
4
4
3
2
2
2
2
Host
Rabbit
572
547
18
6
2
2
2
Clonality
Polyclonal
Conjugate
This BCL2 antibody is un-conjugated
50
50
45
38
35
25
24
16
15
14
14
14
14
14
14
14
13
13
7
7
7
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
833
397
386
338
336
321
232
195
171
81
40
14
14
13
12
11
9
4
3
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN356812
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5552988 EIA WB Goat N-Term Log in to see Polyclonal
1 ABIN5554595 EIA IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN5554596 EIA IF WB Rabbit IgG Intermediate Domain Log in to see Polyclonal
1 ABIN567617 EIA IF WB Mouse IgG2b Log in to see AT1B5
1 ABIN5555618 EIA IF IHC (p) WB Rabbit IgG AA 145-157, Internal Region Log in to see Polyclonal
1 ABIN1105505 EIA FACS IF IHC (p) WB Mouse IgG1 Log in to see 8-00E-012
1 ABIN5566912 EIA RIA Biotin Rabbit AA 1-211 Log in to see Polyclonal
1 ABIN5566910 EIA RIA Rabbit AA 1-211 Log in to see Polyclonal
1 ABIN5541058 EIA Rabbit pThr56 Log in to see Polyclonal
1 ABIN5541059 EIA Rabbit pThr56 Log in to see Polyclonal
1 ABIN5541056 EIA Rabbit Log in to see Polyclonal
1 ABIN5541057 EIA Rabbit Log in to see Polyclonal
1 ABIN2716564 EIA Rabbit IgG Log in to see Polyclonal

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope BH3 Domain
(164), (77), (67), (64), (44), (31), (25), (25), (24), (21), (18), (15), (15), (15), (13), (13), (13), (10), (9), (9), (6), (6), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1053), (258), (213), (54), (28), (22), (18), (18), (5), (4), (4), (3), (2), (2), (2), (2)
Host Rabbit
(572), (547), (18), (6), (2), (2), (2)
Clonality
Polyclonal
Conjugate This BCL2 antibody is un-conjugated
(50), (50), (45), (38), (35), (25), (24), (16), (15), (14), (14), (14), (14), (14), (14), (14), (13), (13), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(833), (397), (386), (338), (336), (321), (232), (195), (171), (81), (40), (14), (14), (13), (12), (11), (9), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN356812) Images
Specificity This antibody is specific to Bcl-2 (BH3 Domain).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the BH3 domain of human Bcl2.
Isotype Ig Fraction

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 antibody (ABIN356812) Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background BCL2 is integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.Synonyms: Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2, BCL2, Bcl-2 alpha
Gene ID 596, 9606
UniProt P10415
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 antibody (ABIN356812) Images back to top
Application Notes ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. Immunoprecipitation: 1/100-1/500Flow Cytometry: 1/100-1/500. Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 antibody (ABIN356812) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-BCL2 antibody (ABIN356812)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Background publications

Liu, Zhou, Li, Sun, Lin, Lang, He, Cao, Yan, Wang, Lu, Han, Cao, Zhang, Zeng: "Modulation of orphan nuclear receptor Nur77-mediated apoptotic pathway by acetylshikonin and analogues." in: Cancer research, Vol. 68, Issue 21, pp. 8871-80, 2008

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN356812) back to top
Supplier Images
 image for anti-B-Cell CLL/lymphoma 2 (BCL2) (BH3 Domain) antibody (ABIN356812) anti-B-Cell CLL/lymphoma 2 (BCL2) (BH3 Domain) antibody
 image for anti-B-Cell CLL/lymphoma 2 (BCL2) (BH3 Domain) antibody (ABIN356812) anti-B-Cell CLL/lymphoma 2 (BCL2) (BH3 Domain) antibody (Image 2)