anti-Human BCL2 antibody for Immunofluorescence

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • Bcl-2
  • PPP1R50
  • bcl-2
  • AW986256
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • BCL-2
  • Bcl2
  • BCL2, apoptosis regulator
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2a
  • B-cell CLL/lymphoma 2 protein
  • BCL2
  • Bcl2
  • bcl2a
  • BCL-2
Epitope
AA 26-75, pThr56
165
77
67
63
44
31
25
25
24
21
18
15
15
15
13
13
13
11
9
9
6
6
6
5
5
5
5
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1063
264
219
54
28
22
18
18
5
4
4
3
2
2
2
2
Host
Rabbit
575
554
18
6
2
2
2
Clonality
Polyclonal
Conjugate
This BCL2 antibody is un-conjugated
53
50
45
41
35
25
24
16
15
14
14
14
14
14
14
14
13
13
7
7
7
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
843
398
386
338
337
321
232
195
171
81
40
15
14
13
12
11
9
4
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN1531787
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.115504 ABIN388095 IF IHC (p) WB Rabbit Ig Fraction AA 75-110, BH3 Domain Log in to see Polyclonal 19
12.115504 ABIN1531491 IF IHC ELISA Rabbit IgG AA 46-95, pThr69 Log in to see Polyclonal
12.115504 ABIN1532505 IF IHC ELISA WB Rabbit IgG AA 46-95 Log in to see Polyclonal
12.115504 ABIN4283376 ELISA FACS ICC IF IHC IHC (p) Mouse IgG1 Log in to see 8E12 1
12.115504 ABIN1538273 IF WB Rabbit Ig Fraction AA 300-327, Center Log in to see Polyclonal
12.115504 ABIN1871251 IF WB Rabbit IgG Log in to see Polyclonal
12.115504 ABIN2705577 IC IF IHC WB Rabbit Center Log in to see Polyclonal
12.115504 ABIN2705579 IC IF IHC WB Rabbit Center Log in to see Polyclonal
12.115504 ABIN2957874 IC IF IP IHC WB Rabbit pSer56 Log in to see Polyclonal
12.115504 ABIN567617 EIA IF WB Mouse IgG2b Log in to see AT1B5
12.115504 ABIN1876758 IF IHC IP WB Rabbit IgG Log in to see Polyclonal
12.115504 ABIN3030102 IF IHC ELISA WB Rabbit Ig Fraction AA 75-110 Log in to see Polyclonal
12.115504 ABIN934065 IF ELISA WB Mouse IgG2b kappa AA 1-211 Log in to see AT1B5
12.115504 ABIN5075260 ICC IF Rabbit IgG Log in to see Polyclonal
12.115504 ABIN1105505 EIA FACS IF IHC (p) WB Mouse IgG1 Log in to see 8-00E-012
12.115504 ABIN2852691 IC IF IHC WB Mouse Log in to see
1 ABIN1105504 IHC (fro) IF IHC (p) WB Mouse IgG1 AA 41-54 Log in to see BL-2 2
1 ABIN968024 IF IP IHC WB Mouse IgG1 AA 49-179 Log in to see 7-Bcl 5
1 ABIN968025 IF IP IHC WB Mouse IgG1 AA 49-179 Log in to see 7-Bcl 5
1 ABIN197202 IF IHC (p) WB Rabbit AA 54-58 Log in to see Polyclonal 4

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope AA 26-75, pThr56
(165), (77), (67), (63), (44), (31), (25), (25), (24), (21), (18), (15), (15), (15), (13), (13), (13), (11), (9), (9), (6), (6), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1063), (264), (219), (54), (28), (22), (18), (18), (5), (4), (4), (3), (2), (2), (2), (2)
Host Rabbit
(575), (554), (18), (6), (2), (2), (2)
Clonality
Polyclonal
Conjugate This BCL2 antibody is un-conjugated
(53), (50), (45), (41), (35), (25), (24), (16), (15), (14), (14), (14), (14), (14), (14), (14), (13), (13), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(843), (398), (386), (338), (337), (321), (232), (195), (171), (81), (40), (15), (14), (13), (12), (11), (9), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN1531787) Images
Specificity

BCL-2 (Phospho-Thr56) Antibody detects endogenous levels of BCL-2 only when phosphorylated at Thr56.

PhosphorylationH:T56

Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human BCL-2 around the phosphorylation site of Thr56.
Isotype IgG

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 antibody (ABIN1531787) Images back to top
Antigen
Alternative Name BCL-2 (BCL2 Antibody Abstract)
Background

Synonyms: Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, BCL2

NCBI Gene Symbol: BCL2

Molecular Weight 26 kDa
Gene ID 10000
OMIM 151430
UniProt P10415
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 antibody (ABIN1531787) Images back to top
Application Notes WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000
Comment

Unigene-Number: Hs.150749 (NCBI Gene Symbol: BCL2)

Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 antibody (ABIN1531787) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20°C for at least 1 year.
Expiry Date 12 months

References for anti-BCL2 antibody (ABIN1531787)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Background publications

Cleary, Smith, Sklar: "Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation." in: Cell, Vol. 47, Issue 1, pp. 19-28, 1986

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN1531787) back to top
Supplier Images
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 26-75), (pThr56) antibody (ABIN1531787) Western blot analysis of extracts from K562 cells treated with H2O2 100uM 30', using ...
Immunohistochemistry (IHC) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 26-75), (pThr56) antibody (ABIN1531787) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BCL-...
Immunofluorescence (IF) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 26-75), (pThr56) antibody (ABIN1531787) Immunofluorescence analysis of A549 cells, using BCL-2 (Phospho-Thr56) Antibody. The ...