anti-Human BCL2 antibody for Immunofluorescence

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • Bcl-2
  • PPP1R50
  • bcl-2
  • AW986256
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • BCL-2
  • Bcl2
  • BCL2, apoptosis regulator
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2a
  • B-cell CLL/lymphoma 2 protein
  • BCL2
  • Bcl2
  • bcl2a
  • BCL-2
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1237
266
253
68
38
21
19
17
6
4
3
3
3
2
2
Host
Rabbit
666
642
19
6
2
Clonality
Polyclonal
Conjugate
This BCL2 antibody is un-conjugated
61
55
48
46
36
23
21
21
18
11
11
11
11
10
10
10
10
10
10
9
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
987
442
440
440
380
357
210
155
142
125
91
42
33
15
13
9
3
3
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN6260222
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
31.012884 ABIN388095 IF IHC (p) WB Rabbit Ig Fraction AA 75-110, BH3 Domain Log in to see Polyclonal 23
23.765488 ABIN934065 IF ELISA WB Mouse IgG2b kappa AA 1-211 Log in to see AT1B5 0
23.512884 ABIN1871251 IF WB Rabbit IgG Log in to see Polyclonal 0
23.512884 ABIN1876758 IF IHC IP WB Rabbit IgG Log in to see Polyclonal 0
19.252602 ABIN3183498 ELISA IF IHC WB Rabbit IgG Ser168, Ser170 Log in to see Polyclonal 0
17.752602 ABIN965651 FACS IF IHC ELISA Mouse IgG1 Log in to see 8E12 3
17.512884 ABIN3030102 IF IHC ELISA WB Rabbit Ig Fraction AA 75-110 Log in to see Polyclonal 0
16.252602 ABIN3181931 ELISA IF IHC IP WB Rabbit IgG pThr56 Log in to see Polyclonal 0
16.252602 ABIN3183499 ELISA IF IHC WB Rabbit IgG Ser172 Log in to see Polyclonal 0
14.512884 ABIN2705577 IC IF IHC WB Rabbit Center Log in to see Polyclonal 0
14.512884 ABIN2705579 IC IF IHC WB Rabbit Center Log in to see Polyclonal 0
14.512884 ABIN1531787 IF IHC IP WB Rabbit IgG AA 26-75, pThr56 Log in to see Polyclonal 1
14.512884 ABIN1532505 IF IHC ELISA WB Rabbit IgG AA 46-95 Log in to see Polyclonal 0
14.512884 ABIN2852691 IC IF IHC WB Mouse Log in to see 0
14.512884 ABIN2957874 IC IF IP IHC WB Rabbit pSer56 Log in to see Polyclonal 0
14.512884 ABIN5648994 ELISA IF IHC (p) WB Rabbit Log in to see Polyclonal 0
14.512884 ABIN5648763 ELISA FACS IF IHC (p) Mouse IgG1 Log in to see 8E12 0
14.512884 ABIN1105505 EIA FACS IF IHC (p) WB Mouse IgG1 Log in to see 8-00E-012 0
14.512884 ABIN1574993 FACS IF ELISA WB Mouse Log in to see 0
14.5 ABIN6260221 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 1

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1237), (266), (253), (68), (38), (21), (19), (17), (6), (4), (3), (3), (3), (2), (2)
Host Rabbit
(666), (642), (19), (6), (2)
Clonality
Polyclonal
Conjugate This BCL2 antibody is un-conjugated
(61), (55), (48), (46), (36), (23), (21), (21), (18), (11), (11), (11), (11), (10), (10), (10), (10), (10), (10), (9), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(987), (442), (440), (440), (380), (357), (210), (155), (142), (125), (91), (42), (33), (15), (13), (9), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 17 references available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN6260222) Images
Specificity BCL-2 Antibody detects endogenous levels of total BCL-2
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Immunogen A synthesized peptide derived from human BCL-2
Isotype IgG
Plasmids, Primers & others

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 antibody (ABIN6260222) Images back to top
Antigen
Alternative Name BCL-2 (BCL2 Antibody Abstract)
Background

Description: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).

Gene: BCL2

Molecular Weight 26kDa
Gene ID 596
UniProt P10415
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 antibody (ABIN6260222) Images back to top
Application Notes WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 antibody (ABIN6260222) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

References for anti-BCL2 antibody (ABIN6260222)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Product cited in:

Zhang, Chen, Wang, Yang, Li, Wang, Liu, Ye: "Treatment of diabetes mellitus-induced erectile dysfunction using endothelial progenitor cells genetically modified with human telomerase reverse transcriptase." in: Oncotarget, Vol. 7, Issue 26, pp. 39302-39315, 2018

Li, Cui, Wang, Wang, Li, Qiu, Yu, Liu, Wen, Rao: "Hyperlipidemia impairs erectile function in rats by causing cavernosal fibrosis." in: Andrologia, Vol. 49, Issue 7, 2018

Wang, Li, Li, Pan, Bai, Huang: "Angelicin inhibits liver cancer growth in vitro and in vivo." in: Molecular medicine reports, Vol. 16, Issue 4, pp. 5441-5449, 2018

Gao, Ma, Ma, Ding, Xu, Wei, Wei, Zhang: "Betulinic acid induces apoptosis and ultrastructural changes in MDA-MB-231 breast cancer cells." in: Ultrastructural pathology, Vol. 42, Issue 1, pp. 49-54, 2018

Yin, Meng, Zhang, Hu, Chen, Han, Wu, You, Li, Jin, Guan: "Bacillus spore-based oral carriers loading curcumin for the therapy of colon cancer." in: Journal of controlled release : official journal of the Controlled Release Society, Vol. 271, pp. 31-44, 2018

Lu, Zhou, Hao, Li, Wang, Shu, Du, Zhu, Zhang, Yin: "The mTOR promotes oxidative stress-induced apoptosis of mesangial cells in diabetic nephropathy." in: Molecular and cellular endocrinology, Vol. 473, pp. 31-43, 2018

Shao, Zhang, Ma, Lu, Meng, Li, Wang, Chen, Zhang, Han, Liu, Ma: "MicroRNA-139-5p affects cisplatin sensitivity in human nasopharyngeal carcinoma cells by regulating the epithelial-to-mesenchymal transition." in: Gene, Vol. 652, pp. 48-58, 2018

Liu, Geng, Zhang, Wang, Zhang, Duan, Zhang: "Oligo-Porphyran Ameliorates Neurobehavioral Deficits in Parkinsonian Mice by Regulating the PI3K/Akt/Bcl-2 Pathway." in: Marine drugs, Vol. 16, Issue 3, 2018

Wang, Liu, Guo, Quan: "Mitofusin-2 Triggers Cervical Carcinoma Cell Hela Apoptosis via Mitochondrial Pathway in Mouse Model." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 46, Issue 1, pp. 69-81, 2018

Zhou, Zhang, Liu, Lei, Liu, Jiao, Dang: "Altered Light Conditions Contribute to Abnormalities in Emotion and Cognition Through HINT1 Dysfunction in C57BL/6 Mice." in: Frontiers in behavioral neuroscience, Vol. 12, pp. 110, 2018

Wang, Li, Chen, Luo, Gu, Wang, Wu, Xu et al.: "Baicalin extracted from Huangqin (Radix Scutellariae Baicalensis) induces apoptosis in gastric cancer cells by regulating B cell lymphoma (Bcl-2)/Bcl-2-associated X protein and activating caspase-3 ..." in: Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan, Vol. 37, Issue 2, pp. 229-5, 2018

Liu, Zheng, Zhang, Wang, Yang, Bai, Dai: "Fucoxanthin Activates Apoptosis via Inhibition of PI3K/Akt/mTOR Pathway and Suppresses Invasion and Migration by Restriction of p38-MMP-2/9 Pathway in Human Glioblastoma Cells." in: Neurochemical research, Vol. 41, Issue 10, pp. 2728-2751, 2017

Zhao, Deng, Xu, Zhang, Mi, Meng, Gou, Xu: "PirB Overexpression Exacerbates Neuronal Apoptosis by Inhibiting TrkB and mTOR Phosphorylation After Oxygen and Glucose Deprivation Injury." in: Cellular and molecular neurobiology, Vol. 37, Issue 4, pp. 707-715, 2017

Chen, Wu, Gan, Liu, Lv, Shen, Dai, Xu: "Iridoid glycoside from Cornus officinalis ameliorated diabetes mellitus-induced testicular damage in male rats: Involvement of suppression of the AGEs/RAGE/p38 MAPK signaling pathway." in: Journal of ethnopharmacology, Vol. 194, pp. 850-860, 2017

Pan, Zhang, Zhao, Zhou, Wang, Deng: "The cardiac glycoside oleandrin induces apoptosis in human colon cancer cells via the mitochondrial pathway." in: Cancer chemotherapy and pharmacology, Vol. 80, Issue 1, pp. 91-100, 2017

Liu, Liu, He, Zhang, Liu, Wang, Qiu, Xu, Xiang, Lv: "Differential gene and lncRNA expression in the lower thoracic spinal cord following ischemia/reperfusion-induced acute kidney injury in rats." in: Oncotarget, Vol. 8, Issue 32, pp. 53465-53481, 2017

Li, Chen, Wang, Wang, Liu: "The effect of cavernous nerve traction on erectile function in rats." in: PLoS ONE, Vol. 12, Issue 10, pp. e0186077, 2017

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN6260222) back to top
Supplier Images
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 2 (BCL2) antibody (ABIN6260222) Western blot analysis of extracts of various sample,using bcl2 antibody.
Immunofluorescence (fixed cells) (IF/ICC) image for anti-B-Cell CLL/lymphoma 2 (BCL2) antibody (ABIN6260222) ABIN6269130 staining A549 cells by ICC/IF. Cells were fixed with PFA and permeabilize...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-B-Cell CLL/lymphoma 2 (BCL2) antibody (ABIN6260222) ABIN6269130 staining MCF7 by IF/ICC. The sample were fixed with PFA and permeabilized...
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 2 (BCL2) antibody (ABIN6260222) Western blot analysis of extracts from rat brain, mouse brain, mouse muscle, rat live...
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 2 (BCL2) antibody (ABIN6260222) Western blot analysis of BCL-2 expression in K562 whole cell lysates,The lane on the ...
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 2 (BCL2) antibody (ABIN6260222) Western blot analysis of extracts from Mouse liver, using BCL-2 Antibody. Lane 1 was ...