anti-Human BCL2 antibody for Immunofluorescence

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • Bcl-2
  • PPP1R50
  • bcl-2
  • AW986256
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • BCL-2
  • Bcl2
  • BCL2, apoptosis regulator
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2a
  • B-cell CLL/lymphoma 2 protein
  • BCL2
  • Bcl2
  • bcl2a
  • BCL-2
Epitope
AA 75-110, BH3 Domain
248
78
64
61
40
28
28
26
22
21
16
15
15
15
11
10
9
9
9
8
8
7
7
7
7
6
5
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1257
273
251
70
36
21
21
19
6
4
3
3
3
2
2
Host
Rabbit
702
632
19
6
2
Clonality
Polyclonal
Conjugate
This BCL2 antibody is un-conjugated
62
57
53
50
43
25
24
24
23
15
14
14
14
14
14
14
13
13
11
11
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
6
6
6
6
6
6
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1028
473
473
424
409
406
224
188
156
155
81
42
33
15
13
9
6
3
2
2
1
1
1
1
1
1
Options
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Catalog No. ABIN388095
$ 335.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.108023 ABIN2705577 IC IF IHC WB Rabbit Center Log in to see Polyclonal 0
12.108023 ABIN2705579 IC IF IHC WB Rabbit Center Log in to see Polyclonal 0
12.108023 ABIN1531787 IF IHC IP WB Rabbit IgG AA 26-75, pThr56 Log in to see Polyclonal 1
12.108023 ABIN1871251 IF WB Rabbit IgG Log in to see Polyclonal 0
12.108023 ABIN1532505 IF IHC ELISA WB Rabbit IgG AA 46-95 Log in to see Polyclonal 0
12.108023 ABIN1531491 IF IHC ELISA Rabbit IgG AA 46-95, pThr69 Log in to see Polyclonal 0
12.108023 ABIN1876758 IF IHC IP WB Rabbit IgG Log in to see Polyclonal 0
12.108023 ABIN1538273 IF WB Rabbit Ig Fraction AA 300-327, Center Log in to see Polyclonal 0
12.108023 ABIN4283376 ELISA FACS ICC IF IHC IHC (p) Mouse IgG1 Log in to see 8E12 1
12.108023 ABIN3030102 IF IHC ELISA WB Rabbit Ig Fraction AA 75-110 Log in to see Polyclonal 0
12.108023 ABIN2852691 IC IF IHC WB Mouse Log in to see 0
12.108023 ABIN2957874 IC IF IP IHC WB Rabbit pSer56 Log in to see Polyclonal 0
12.108023 ABIN934065 IF ELISA WB Mouse IgG2b kappa AA 1-211 Log in to see AT1B5 0
12.108023 ABIN567617 EIA IF WB Mouse IgG2b Log in to see AT1B5 0
12.108023 ABIN5075260 ICC IF Rabbit IgG Log in to see Polyclonal 0
12.108023 ABIN1105505 EIA FACS IF IHC (p) WB Mouse IgG1 Log in to see 8-00E-012 0
12.108023 ABIN5648994 ELISA IF IHC (p) WB Rabbit Log in to see Polyclonal 0
12.108023 ABIN5649330 ELISA IF WB Mouse IgG2b kappa Log in to see AT1B5 0
12.108023 ABIN5648763 ELISA FACS IF IHC (p) Mouse IgG1 Log in to see 8E12 0
1 ABIN4283388 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa Log in to see 8C8 0

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope AA 75-110, BH3 Domain
(248), (78), (64), (61), (40), (28), (28), (26), (22), (21), (16), (15), (15), (15), (11), (10), (9), (9), (9), (8), (8), (7), (7), (7), (7), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1257), (273), (251), (70), (36), (21), (21), (19), (6), (4), (3), (3), (3), (2), (2)
Host Rabbit
(702), (632), (19), (6), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This BCL2 antibody is un-conjugated
(62), (57), (53), (50), (43), (25), (24), (24), (23), (15), (14), (14), (14), (14), (14), (14), (13), (13), (11), (11), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1028), (473), (473), (424), (409), (406), (224), (188), (156), (155), (81), (42), (33), (15), (13), (9), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 19 references available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN388095) Images
Specificity This Bcl-2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85~115 amino acids from the BH3 domain of human Bcl2.
Predicted Reactivity Cow (Bovine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This Bcl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-110 AA from human Bcl.
Clone RB04684
Isotype Ig Fraction
Plasmids, Primers & others

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 antibody (ABIN388095) Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background BCL2 is integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.
Synonyms: Apoptosis regulator Bcl-2, B-cell lymphoma protein 2
Pathways: Neurotrophin signaling pathway, Toxoplasmosis, Pathways in cancer, Protein processing in endoplasmic reticulum, Tuberculosis, Focal adhesion, Prostate cancer, Small cell lung cancer, Apoptosis, Colorectal cancer, Amyotrophic lateral sclerosis (ALS)
Molecular Weight 26266 Da
Gene ID 596
UniProt P10415
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 antibody (ABIN388095) Images back to top
Application Notes IHC (p) = 1:10-50, IF = 1:10-50, WB = 1:1000
Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 antibody (ABIN388095) Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-BCL2 antibody (ABIN388095)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Product cited in:

Kiss, Tóth, Sarang, Garabuczi, Szondy: "Retinoids induce Nur77-dependent apoptosis in mouse thymocytes." in: Biochimica et biophysica acta, Vol. 1853, Issue 3, pp. 660-70, 2015

Hu, Baldwin: "Differential roles for Bim and Nur77 in thymocyte clonal deletion induced by ubiquitous self-antigen." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 6, pp. 2643-53, 2015

Cai, Xia, Yang, Liu, Shao, Liu, Yang, Xiong: "Ropivacaine- and bupivacaine-induced death of rabbit annulus fibrosus cells in vitro: involvement of the mitochondrial apoptotic pathway." in: Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society, 2015

Li, Wang, Wang, Gao, Zhang, Lu: "Oridonin phosphate-induced autophagy effectively enhances cell apoptosis of human breast cancer cells." in: Medical oncology (Northwood, London, England), Vol. 32, Issue 1, pp. 365, 2014

Pickles, Destroismaisons, Peyrard, Cadot, Rouleau, Brown, Julien, Arbour, Vande Velde: "Mitochondrial damage revealed by immunoselection for ALS-linked misfolded SOD1." in: Human molecular genetics, Vol. 22, Issue 19, pp. 3947-59, 2013

Chiavarina, Whitaker-Menezes, Martinez-Outschoorn, Witkiewicz, Birbe, Howell, Pestell, Smith, Daniel, Sotgia, Lisanti: "Pyruvate kinase expression (PKM1 and PKM2) in cancer-associated fibroblasts drives stromal nutrient production and tumor growth." in: Cancer biology & therapy, Vol. 12, Issue 12, pp. 1101-13, 2012

Zhao, He, Liu, Zhu, Tian, Gao, He, Yue, Lei, Ni, Wang, Jin, Hao, Lin, Chen: "Natural diterpenoid compound elevates expression of Bim protein, which interacts with antiapoptotic protein Bcl-2, converting it to proapoptotic Bax-like molecule." in: The Journal of biological chemistry, Vol. 287, Issue 2, pp. 1054-65, 2012

Guareschi, Cova, Cereda, Ceroni, Donetti, Bosco, Trotti, Pasinelli: "An over-oxidized form of superoxide dismutase found in sporadic amyotrophic lateral sclerosis with bulbar onset shares a toxic mechanism with mutant SOD1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 13, pp. 5074-9, 2012

Pedrini, Sau, Guareschi, Bogush, Brown, Naniche, Kia, Trotti, Pasinelli: "ALS-linked mutant SOD1 damages mitochondria by promoting conformational changes in Bcl-2." in: Human molecular genetics, Vol. 19, Issue 15, pp. 2974-86, 2010

Ferlini, Cicchillitti, Raspaglio, Bartollino, Cimitan, Bertucci, Mozzetti, Gallo, Persico, Fattorusso, Campiani, Scambia: "Paclitaxel directly binds to Bcl-2 and functionally mimics activity of Nur77." in: Cancer research, Vol. 69, Issue 17, pp. 6906-14, 2009

Deng, Gao, May: "Protein phosphatase 2A inactivates Bcl2's antiapoptotic function by dephosphorylation and up-regulation of Bcl2-p53 binding." in: Blood, Vol. 113, Issue 2, pp. 422-8, 2009

Kolluri, Zhu, Zhou, Lin, Chen, Sun, Tian, Town, Cao, Lin, Zhai, Kitada, Luciano, ODonnell, Cao, He, Lin, Reed, Satterthwait, Zhang: "A short Nur77-derived peptide converts Bcl-2 from a protector to a killer." in: Cancer cell, Vol. 14, Issue 4, pp. 285-98, 2008

Thompson, Winoto: "During negative selection, Nur77 family proteins translocate to mitochondria where they associate with Bcl-2 and expose its proapoptotic BH3 domain." in: The Journal of experimental medicine, Vol. 205, Issue 5, pp. 1029-36, 2008

Del Gaizo Moore, Schlis, Sallan, Armstrong, Letai: "BCL-2 dependence and ABT-737 sensitivity in acute lymphoblastic leukemia." in: Blood, Vol. 111, Issue 4, pp. 2300-9, 2008

Liu, Zhou, Li, Sun, Lin, Lang, He, Cao, Yan, Wang, Lu, Han, Cao, Zhang, Zeng: "Modulation of orphan nuclear receptor Nur77-mediated apoptotic pathway by acetylshikonin and analogues." in: Cancer research, Vol. 68, Issue 21, pp. 8871-80, 2008

Lei, Chen, Du, Yu, Wang, Qu, Xia, He, Mao, Zong, Liao, Mehrpour, Hao, Chen: "Gossypol induces Bax/Bak-independent activation of apoptosis and cytochrome c release via a conformational change in Bcl-2." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 12, pp. 2147-9, 2006

Background publications

Zheng, Zhou, Ye, Li, Bai, Zhang: "The conformation change of Bcl-2 is involved in arsenic trioxide-induced apoptosis and inhibition of proliferation in SGC7901 human gastric cancer cells." in: World journal of surgical oncology, Vol. 8, Issue 1, pp. 31, 2010

Thoulouze, Lafage, Yuste, Baloul, Edelman, Kroemer, Israel, Susin, Lafon: "High level of Bcl-2 counteracts apoptosis mediated by a live rabies virus vaccine strain and induces long-term infection." in: Virology, Vol. 314, Issue 2, pp. 549-61, 2003

Iwata, Stevenson, Minard, Tasch, Tupper, Lagasse, Weissman, Harlan, Winn: "Over-expression of Bcl-2 provides protection in septic mice by a trans effect." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 6, pp. 3136-41, 2003

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN388095) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 75-110), (BH3 Domain) antibody (ABIN388095) Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Bcl-2 ...
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 75-110), (BH3 Domain) antibody (ABIN388095) Left panel, analysis of Bcl-2 BH3 domain exposure in HEK293 cells transfected with a ...
Immunofluorescence (IF) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 75-110), (BH3 Domain) antibody (ABIN388095) Confocal immunofluorescent analysis of Bcl-2 Antibody (BH3 Domain Specific) (ABIN3880...
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 75-110), (BH3 Domain) antibody (ABIN388095) Anti-Bcl- 2 Antibody (BH3) at 1:2000 dilution + THP-1 whole cell lysate Lysates/prote...