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|Antigen||B-Cell CLL/lymphoma 2 (BCL2) Antibodies|
|Epitope||AA 61-76 Alternatives|
|Reactivity||Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This BCL2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|12 references available|
|Supplier||Log in to see|
Product Details anti-BCL2 AntibodyTarget Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN371613) Images
|Specificity||This antibody reacts with Bcl-2 (Mr. 26 kDa). The monoclonal antibody 10C2 reacts with an epitope between amino acids 61-76 of Mouse Bcl-2. The antibody also reacts with Rat Bcl-2 (Ref.1,14).|
|Characteristics||Synonyms: BCL2, Bcl-2 alpha, Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2|
|Immunogen||KLH-conjugated peptide corresponding to 61-76 amino acid sequence of murine Bcl-2. (Ref. 1)|
Target Details BCL2Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 antibody (ABIN371613) Images back to top
|Alternative Name||Bcl-2 (BCL2 Antibody Abstract)|
|Background||Bcl-2 is a 26 kDa member of the family of proteins involved in regulation of programmed cell death, or apoptosis. It is expressed in a variety of both normal and neoplastic tissues and appears to be membranebound since it is detectable in crude membrane and nuclear fractions, but not in the soluble fraction of cell lysates. Overexpression of Bcl-2 can prevent apoptosis, while formation of heterodimers with Bax, another Bcl-2-related protein, inhibits Bcl-2's ability to promote cell survival. Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellular systems. (Ref.2) It plays a key role in tissue and organ development as well as in adult tissues during cell turnover. Apoptosis can be induced by a variety of internal and external stimuli including growth factor deprivation, cytokine treatment, antigen-receptor engagement, cell-cell interactions, irradiation and glucocorticoid treatment. (Ref.3) Bcl-2 is a widely studied protein that has been shown to be a potent inhibitor of programmed cell death. It has been localized to the outer mitochondrial membrane, perinuclear membrane, and endoplasmic reticulum. Bcl-2 is expressed in memory and resting, or other long-lived lymphoid cells, follicular mantle cells, medullary thymocytes, and lymphomas. Germical center cells and cortical thymocytes are negative for Bcl-2. Upregulation of Bcl-2 prevents or delays apoptosis induced by a variety of stimuli, including growth factor deprivation, gamma-irradiation, glucocorticoids, and chemotherapeutic agents. During lymphoid development, expression of the Bcl-2 protein appears to be regulated in a stage-specific manner, and is thought to be a survival signal for positive selection.Synonyms: Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2, BCL2, Bcl-2 alpha|
|Pathways||MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling|
Application DetailsProduct Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 antibody (ABIN371613) Images back to top
ELISA. Western Blot: < / = 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 antibody (ABIN371613) Images back to top
|Buffer||100 mM Borate buffered saline, pH 8.2 without preservatives or amine-containing buffer salts.|
Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-BCL2 antibody (ABIN371613)Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Gottschalk, Boise, Oltvai, Accavitti, Korsmeyer, Quintáns, Thompson: "The ability of Bcl-x(L) and Bcl-2 to prevent apoptosis can be differentially regulated." in: Cell death and differentiation, Vol. 3, Issue 1, pp. 113-8, 2006
Nuñez, Clarke: "The Bcl-2 family of proteins: regulators of cell death and survival." in: Trends in cell biology, Vol. 4, Issue 11, pp. 399-403, 2004
Hsu, Youle: "Nonionic detergents induce dimerization among members of the Bcl-2 family." in: The Journal of biological chemistry, Vol. 272, Issue 21, pp. 13829-34, 1997
Cheng, Levine, Boise, Thompson, Hardwick: "Bax-independent inhibition of apoptosis by Bcl-XL." in: Nature, Vol. 379, Issue 6565, pp. 554-6, 1996
Cory: "Regulation of lymphocyte survival by the bcl-2 gene family." in: Annual review of immunology, Vol. 13, pp. 513-43, 1995
Farrow, White, Martinou, Raven, Pun, Grinham, Martinou, Brown: "Cloning of a bcl-2 homologue by interaction with adenovirus E1B 19K." in: Nature, Vol. 374, Issue 6524, pp. 731-3, 1995
Chittenden, Harrington, OConnor, Flemington, Lutz, Evan, Guild: "Induction of apoptosis by the Bcl-2 homologue Bak." in: Nature, Vol. 374, Issue 6524, pp. 733-6, 1995
Kiefer, Brauer, Powers, Wu, Umansky, Tomei, Barr: "Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak." in: Nature, Vol. 374, Issue 6524, pp. 736-9, 1995
Shimizu, Eguchi, Kosaka, Kamiike, Matsuda, Tsujimoto: "Prevention of hypoxia-induced cell death by Bcl-2 and Bcl-xL." in: Nature, Vol. 374, Issue 6525, pp. 811-3, 1995
Yin, Oltvai, Korsmeyer: "BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax." in: Nature, Vol. 369, Issue 6478, pp. 321-3, 1994
Oltvai, Milliman, Korsmeyer: "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death." in: Cell, Vol. 74, Issue 4, pp. 609-19, 1993
Cohen: "Programmed cell death in the immune system." in: Advances in immunology, Vol. 50, pp. 55-85, 1991