anti-Pig (Porcine) BCL2 antibody for Immunofluorescence

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • Bcl-2
  • PPP1R50
  • bcl-2
  • AW986256
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • BCL-2
  • Bcl2
  • BCL2, apoptosis regulator
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2a
  • B-cell CLL/lymphoma 2 protein
  • BCL2
  • Bcl2
  • bcl2a
  • BCL-2
Reactivity
Human, Pig (Porcine), Primate
1257
273
251
69
36
21
20
19
6
4
3
3
3
2
2
Host
Mouse
701
633
19
6
2
Clonality
Monoclonal
Conjugate
This BCL2 antibody is un-conjugated
62
57
53
50
43
25
24
24
23
15
14
14
14
14
14
14
13
13
11
11
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
6
6
6
6
6
6
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1028
473
472
423
409
406
224
187
155
155
81
42
33
15
13
9
6
3
2
2
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN4283388
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4283406 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB HRP Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283394 ELISA ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN6174804 FACS IF IHC (f) WB CF®660R Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174792 FACS IF IHC (f) WB CF®405S Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174794 FACS IF IHC (f) WB CF405M Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174802 FACS IF IHC (f) WB CF®555 Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174806 FACS IF IHC (f) WB CF®568 Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174810 FACS IF IHC (f) WB CF®680 Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174816 FACS IF IHC (f) WB CF®594 Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174800 FACS IF IHC (f) WB CF®647 Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174812 FACS IF IHC (f) WB CF®680R Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174798 FACS IF IHC (f) WB CF®543 Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174796 FACS IF IHC (f) WB CF®640R Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174814 FACS IF IHC (f) WB CF®488A Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174808 FACS IF IHC (f) WB CF®770 Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN3026592 FACS IF IHC (p) WB Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174827 FACS IF IHC (f) WB Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174818 FACS IF IHC (f) WB APC Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174821 FACS IF IHC (f) WB Biotin Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174825 FACS IF IHC (f) WB PerCP Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Reactivity Human, Pig (Porcine), Primate
(1257), (273), (251), (69), (36), (21), (20), (19), (6), (4), (3), (3), (3), (2), (2)
Host Mouse
(701), (633), (19), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BCL2 antibody is un-conjugated
(62), (57), (53), (50), (43), (25), (24), (24), (23), (15), (14), (14), (14), (14), (14), (14), (13), (13), (11), (11), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1028), (473), (472), (423), (409), (406), (224), (187), (155), (155), (81), (42), (33), (15), (13), (9), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling Images
Specificity This antibody recognizes a protein of 25-26 kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Protein A or G purified
Immunogen A synthetic peptide corresponding to amino acids 41-60 of human Bcl-2 was used as immunogen to generate the antibody, GenBank no. NP_000648.2. This sequence is 100% conserved in the human alpha (239 aa) and beta (205 aa) isoforms.
Clone 8C8
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background Gene Symbol: BCL2
Molecular Weight Theoretical MW: 25 kDa
Gene ID 596
UniProt P10415
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling Images back to top
Application Notes Western Blot 0.5-3 μg/mL, Flow Cytometry 0.5-1 μg/million cells, Immunohistochemistry 0.5-1 μg/mL, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunohistochemistry-Paraffin 0.5-1 μg/mL, Immunohistochemistry-Frozen 0.5-1 μg/mLUse in Flow Cytometry and Immunocytochemistry/Immunofluorescence reported in literature. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1 mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Images back to top
Format Liquid
Buffer PBS with 0.05 % BSA.
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C.

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-B-Cell CLL/lymphoma 2 (BCL2) antibody (ABIN4283388) Flow Cytometry: Bcl-2 Antibody (8C8) [NBP2-29630] - Analysis using the HRP conjugate ...
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 2 (BCL2) antibody (ABIN4283388) Western Blot: Bcl-2 Antibody (8C8) [NBP2-29630] - Analysis using the Azide and BSA Fr...
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 2 (BCL2) antibody (ABIN4283388) Western Blot: Bcl-2 Antibody (8C8) [NBP2-29630] - Analysis of Bcl-2 in human A) HeLa,...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-B-Cell CLL/lymphoma 2 (BCL2) antibody (ABIN4283388) Immunohistochemistry-Paraffin: Bcl-2 Antibody (8C8) [NBP2-29630] - Formalin-fixed, pa...