anti-Pig (Porcine) BCL2 antibody for Immunofluorescence

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • Bcl-2
  • PPP1R50
  • bcl-2
  • AW986256
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • BCL-2
  • Bcl2
  • BCL2, apoptosis regulator
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2a
  • B-cell CLL/lymphoma 2 protein
  • BCL2
  • Bcl2
  • bcl2a
  • BCL-2
Reactivity
Human, Pig (Porcine), Primate
1068
264
220
51
26
21
19
16
5
4
3
3
3
2
2
2
Host
Mouse
577
557
18
6
2
2
2
Clonality
Monoclonal
Conjugate
This BCL2 antibody is un-conjugated
52
51
45
41
35
25
24
16
15
15
14
14
14
14
14
14
13
13
7
7
7
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
848
421
390
339
338
308
234
196
169
81
40
15
14
13
12
11
9
3
3
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4283388
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN337080 FACS IF IHC (p) ELISA WB Mouse IgG1 AA 41-54 Log in to see 0
1 ABIN4283406 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB HRP Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283394 ELISA ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN3026592 FACS IF IHC (p) WB Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN386113 FACS IF IHC (p) WB Mouse IgG1 AA 41-54 Log in to see 8C8 0
1 ABIN4283386 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283400 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283402 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Biotin Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283405 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283387 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283397 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283403 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283399 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283407 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283411 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283408 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283412 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 405 Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283398 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283404 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283410 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 550 Mouse IgG1 kappa Log in to see 8C8 0

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Reactivity Human, Pig (Porcine), Primate
(1068), (264), (220), (51), (26), (21), (19), (16), (5), (4), (3), (3), (3), (2), (2), (2)
Host Mouse
(577), (557), (18), (6), (2), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BCL2 antibody is un-conjugated
(52), (51), (45), (41), (35), (25), (24), (16), (15), (15), (14), (14), (14), (14), (14), (14), (13), (13), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(848), (421), (390), (339), (338), (308), (234), (196), (169), (81), (40), (15), (14), (13), (12), (11), (9), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling Images
Specificity This antibody recognizes a protein of 25-26 kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Protein A or G purified
Immunogen A synthetic peptide corresponding to amino acids 41-60 of human Bcl-2 was used as immunogen to generate the antibody, GenBank no. NP_000648.2. This sequence is 100% conserved in the human alpha (239 aa) and beta (205 aa) isoforms.
Clone 8C8
Isotype IgG1 kappa

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background Gene Symbol: BCL2
Molecular Weight Theoretical MW: 25 kDa
Gene ID 596
UniProt P10415
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling Images back to top
Application Notes Western Blot 0.5-3 μg/mL, Flow Cytometry 0.5-1 μg/million cells, Immunohistochemistry 0.5-1 μg/mL, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunohistochemistry-Paraffin 0.5-1 μg/mL, Immunohistochemistry-Frozen 0.5-1 μg/mLUse in Flow Cytometry and Immunocytochemistry/Immunofluorescence reported in literature. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1 mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Images back to top
Format Liquid
Buffer PBS with 0.05 % BSA.
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C.

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-B-Cell CLL/lymphoma 2 (BCL2) antibody (ABIN4283388) Flow Cytometry: Bcl-2 Antibody (8C8) [NBP2-29630] - Analysis using the HRP conjugate ...
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 2 (BCL2) antibody (ABIN4283388) Western Blot: Bcl-2 Antibody (8C8) [NBP2-29630] - Analysis using the Azide and BSA Fr...
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 2 (BCL2) antibody (ABIN4283388) Western Blot: Bcl-2 Antibody (8C8) [NBP2-29630] - Analysis of Bcl-2 in human A) HeLa,...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-B-Cell CLL/lymphoma 2 (BCL2) antibody (ABIN4283388) Immunohistochemistry-Paraffin: Bcl-2 Antibody (8C8) [NBP2-29630] - Formalin-fixed, pa...