anti-Pig (Porcine) BCL2 antibody for Immunofluorescence

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • Bcl-2
  • PPP1R50
  • bcl-2
  • AW986256
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • BCL-2
  • Bcl2
  • BCL2, apoptosis regulator
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2a
  • B-cell CLL/lymphoma 2 protein
  • BCL2
  • Bcl2
  • bcl2a
  • BCL-2
Epitope
AA 41-54
246
80
64
44
40
29
28
27
21
17
15
15
15
11
10
10
10
9
9
9
8
7
7
7
7
6
5
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey, Pig (Porcine)
1237
267
254
67
37
21
19
17
6
4
3
3
3
2
2
Host
Mouse
667
641
19
6
2
Clonality
Monoclonal
Conjugate
This BCL2 antibody is conjugated to PerCP
61
55
48
46
36
23
21
21
17
11
11
11
11
10
10
10
10
10
10
9
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
987
441
441
440
381
357
210
154
143
125
91
42
33
15
13
9
3
3
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN6174825
$ 392.62
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN6174826 FACS IF IHC (f) WB PE Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
7 ABIN6174818 FACS IF IHC (f) WB APC Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
7 ABIN6174829 FACS IF IHC (f) WB Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
7 ABIN3026592 FACS IF IHC (p) WB Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174796 FACS IF IHC (f) WB CF®640R Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174802 FACS IF IHC (f) WB CF®555 Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174804 FACS IF IHC (f) WB CF®660R Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174806 FACS IF IHC (f) WB CF®568 Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174814 FACS IF IHC (f) WB CF®488A Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174800 FACS IF IHC (f) WB CF®647 Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174810 FACS IF IHC (f) WB CF®680 Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174808 FACS IF IHC (f) WB CF®770 Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174812 FACS IF IHC (f) WB CF®680R Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174816 FACS IF IHC (f) WB CF®594 Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174792 FACS IF IHC (f) WB CF®405S Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174794 FACS IF IHC (f) WB CF405M Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174798 FACS IF IHC (f) WB CF®543 Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174827 FACS IF IHC (f) WB Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174821 FACS IF IHC (f) WB Biotin Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0
1 ABIN6174819 FACS IF IHC (f) WB Alkaline Phosphatase (AP) Mouse IgG1, kappa AA 41-54 Log in to see 8C8 0

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope AA 41-54
(246), (80), (64), (44), (40), (29), (28), (27), (21), (17), (15), (15), (15), (11), (10), (10), (10), (9), (9), (9), (8), (7), (7), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey, Pig (Porcine)
(1237), (267), (254), (67), (37), (21), (19), (17), (6), (4), (3), (3), (3), (2), (2)
Host Mouse
(667), (641), (19), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BCL2 antibody is conjugated to PerCP
(61), (55), (48), (46), (36), (23), (21), (21), (17), (11), (11), (11), (11), (10), (10), (10), (10), (10), (10), (9), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
(987), (441), (441), (440), (381), (357), (210), (154), (143), (125), (91), (42), (33), (15), (13), (9), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling Images
Purpose Mouse Monoclonal anti-Bcl-2 (8C8), Per-CP Conjugate
Characteristics This antibody recognizes a protein of 25-26 kDa, identified as the bcl-2 α oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 α oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 α protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.
Clone 8C8
Isotype IgG1, kappa
Plasmids, Primers & others

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background Apoptosis regulator Bcl-2, B-cell CLL/lymphoma-2
Molecular Weight 25-26 kDa
Gene ID 596, 150749
UniProt P10415
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling Images back to top
Application Notes Does not react with mouse or rat, others not tested
  • Immunohistology formalin-fixed 0.5-1 μg/mL
  • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Immunofluorescence 1-2 μg/mL
  • Western blotting 0.5-1 μg/mL
  • Optimal dilution for a specific application should be determined by user
Comment

Jurkat, K562, HL-60, or HeLa Cells. Tonsil or follicular lymphomas.

Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Images back to top
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling back to top
Supplier Images
 image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody (PerCP) (ABIN6174825) anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody (PerCP)
 image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody (PerCP) (ABIN6174825) anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody (PerCP) (Image 2)