anti-Horse (Equine) BRAF antibody for Western Blotting

Recommended BRAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
  • B-RAF1
  • BRAF1
  • NS7
  • RAFB1
  • C-RMIL
  • RMIL
  • B-Raf
  • braf1
  • rafb1
  • b-raf1
  • sierrabraf
  • BRAF
  • 9930012E13Rik
  • AA120551
  • AA387315
  • AA473386
  • B-raf
  • Braf-2
  • Braf2
  • C230098H17
  • C87398
  • D6Ertd631e
  • braf
  • fc48g01
  • wu:fc48g01
  • B-Raf proto-oncogene, serine/threonine kinase
  • B-Raf proto-oncogene, serine/threonine kinase S homeolog
  • Braf transforming gene
  • si:dkey-222f8.3
  • BRAF
  • Braf
  • braf.S
  • braf
  • si:dkey-222f8.3
Epitope
AA 754-765
36
33
29
24
22
21
21
18
16
13
11
10
10
9
8
6
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Horse (Equine), Human, Monkey
406
84
68
12
8
3
3
1
1
1
1
1
Host
Rabbit
309
95
3
Clonality
Polyclonal
Conjugate
This BRAF antibody is un-conjugated
20
20
20
17
17
17
3
3
3
3
3
3
3
3
3
3
3
Application
Western Blotting (WB)
266
211
145
78
70
45
39
22
11
7
5
4
1
1
1
1
Supplier
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Catalog No. ABIN205677
$ 639.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2617714 WB Rabbit IgG AA 344-362 Log in to see Polyclonal

General

Antigen V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
Epitope AA 754-765
(36), (33), (29), (24), (22), (21), (21), (18), (16), (13), (11), (10), (10), (9), (8), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Horse (Equine), Human, Monkey
(406), (84), (68), (12), (8), (3), (3), (1), (1), (1), (1), (1)
Host Rabbit
(309), (95), (3)
Clonality
Polyclonal
Conjugate This BRAF antibody is un-conjugated
(20), (20), (20), (17), (17), (17), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Western Blotting (WB)
(266), (211), (145), (78), (70), (45), (39), (22), (11), (7), (5), (4), (1), (1), (1), (1)
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Product Details anti-BRAF Antibody

Target Details BRAF Application Details Handling Images
Specificity Recognizes full length recombinant human BRaf (plus 6His tag, Mr ~94kD) and B-Raf splice variants in cell lysates (Mr ~68-94kD). Species cross-reactivity: Rat and mouse.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Horse, Opossum (100%) Mouse, Porcine, Turkey, Rabbit, Chicken, Platypus (92%) Bat (83%).
Purification Protein A purified
Immunogen Synthetic peptide corresponding to amino acids 754-765 (C-IQAGGYGAFPVH) of human B-Raf, with an N-terminal cysteine added for conjugation to KLH. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Horse, Opossum (100%), Mouse, Porcine, Turkey, Rabbit, Chicken, Platypus (92%), Bat (83%).

Type of Immunogen: Synthetic peptide - KLH conjugated
Isotype IgG

Target Details BRAF

Product Details anti-BRAF Antibody Application Details Handling Images back to top
Antigen
Alternative Name BRAF / B-Raf (BRAF Antibody Abstract)
Background Name/Gene ID: BRAF
Subfamily: Raf
Family: Protein Kinase

Synonyms: BRAF, 94 kDa B-raf protein, B-raf, BRAF1, B Raf, Proto-oncogene B-Raf, p94, B-RAF1, NS7, ONCOGENE BRAF, RAFB1
Gene ID 673
UniProt P15056
Pathways MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy

Application Details

Product Details anti-BRAF Antibody Target Details BRAF Handling Images back to top
Application Notes Approved: WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, MAP Kinase Activation and MBP Phosphorylation assays. Western Blot: 1-2 μg/mL detects B-Raf splice variants in RIPA lysates from PC12 cells and the full length purified B-Raf, active enzyme. MAP Kinase Activation and MBP Phosphorylation: 4 μg.
Restrictions For Research Use only

Handling

Product Details anti-BRAF Antibody Target Details BRAF Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide 30 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.