anti-Human BRAF antibody for Dot Blot

Recommended BRAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
  • B-RAF1
  • BRAF1
  • NS7
  • RAFB1
  • C-RMIL
  • RMIL
  • B-Raf
  • braf1
  • rafb1
  • b-raf1
  • sierrabraf
  • BRAF
  • 9930012E13Rik
  • AA120551
  • AA387315
  • AA473386
  • B-raf
  • Braf-2
  • Braf2
  • C230098H17
  • C87398
  • D6Ertd631e
  • braf
  • fc48g01
  • wu:fc48g01
  • B-Raf proto-oncogene, serine/threonine kinase
  • B-Raf proto-oncogene, serine/threonine kinase S homeolog
  • Braf transforming gene
  • si:dkey-222f8.3
  • BRAF
  • Braf
  • braf.S
  • braf
  • si:dkey-222f8.3
Epitope
pThr400
36
33
28
24
22
21
21
18
16
13
11
10
10
9
8
6
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
406
84
68
13
9
3
3
2
1
1
1
1
Host
Rabbit
309
95
3
Clonality
Polyclonal
Conjugate
This BRAF antibody is un-conjugated
20
20
20
17
17
17
3
3
3
3
3
3
3
3
3
3
3
Application
Dot Blot (DB)
273
220
150
81
70
47
39
25
11
7
5
4
1
1
1
1
Options
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Catalog No. ABIN389897
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
28.705544 ABIN389701 DB Rabbit Ig Fraction pThr439 Log in to see Polyclonal 2
28.705544 ABIN389803 DB Rabbit Ig Fraction pSer445 Log in to see Polyclonal 0
28.705544 ABIN389791 DB Rabbit Ig Fraction pThr598 Log in to see Polyclonal 0
28.705544 ABIN3029547 DB ELISA Rabbit Ig Fraction pSer445 Log in to see Polyclonal 0
28.705544 ABIN3029548 DB ELISA Rabbit Ig Fraction pThr400 Log in to see Polyclonal 0
28.705544 ABIN3029549 DB ELISA Rabbit Ig Fraction pThr439 Log in to see Polyclonal 0
1 ABIN1958558 DB ELISA PE Rabbit IgG pSer445 Log in to see Polyclonal 0
1 ABIN1958596 DB ELISA FACS IHC IP WB PE Rabbit IgG pSer601 Log in to see Polyclonal 0
1 ABIN1955095 DB ELISA FACS IHC IP WB Biotin Rabbit IgG pSer601 Log in to see Polyclonal 0
1 ABIN1955243 DB ELISA Biotin Rabbit IgG pThr400 Log in to see Polyclonal 0
1 ABIN1955247 DB ELISA WB Biotin Rabbit IgG pThr439 Log in to see Polyclonal 0
1 ABIN1955261 DB ELISA Biotin Rabbit IgG pThr598 Log in to see Polyclonal 0
1 ABIN1956227 DB ELISA FITC Rabbit IgG pSer445 Log in to see Polyclonal 0
1 ABIN1956419 DB ELISA FITC Rabbit IgG pThr400 Log in to see Polyclonal 0
1 ABIN1956423 DB ELISA WB FITC Rabbit IgG pThr439 Log in to see Polyclonal 0
1 ABIN1956264 DB ELISA FACS IHC IP WB FITC Rabbit IgG pSer601 Log in to see Polyclonal 0
1 ABIN1956438 DB ELISA FITC Rabbit IgG pThr598 Log in to see Polyclonal 0
1 ABIN1953114 DB ELISA WB Alkaline Phosphatase (AP) Rabbit IgG pThr439 Log in to see Polyclonal 0
1 ABIN1953131 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG pThr598 Log in to see Polyclonal 0
1 ABIN1953110 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG pThr400 Log in to see Polyclonal 0

General

Antigen V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
Epitope pThr400
(36), (33), (28), (24), (22), (21), (21), (18), (16), (13), (11), (10), (10), (9), (8), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(406), (84), (68), (13), (9), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(309), (95), (3)
Clonality (Clone)
Polyclonal   ( )
Conjugate This BRAF antibody is un-conjugated
(20), (20), (20), (17), (17), (17), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Dot Blot (DB)
(273), (220), (150), (81), (70), (47), (39), (25), (11), (7), (5), (4), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-BRAF Antibody

Target Details BRAF Application Details Handling References for anti-BRAF antibody (ABIN389897) Images
Specificity This Phospho-BRAF-pT400 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T400 of human BRAF.
Predicted Reactivity Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This BRAF Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T400 of human BRAF.
Clone RB13721
Isotype Ig Fraction

Target Details BRAF

Product Details anti-BRAF Antibody Application Details Handling References for anti-BRAF antibody (ABIN389897) Images back to top
Antigen
Alternative Name BRAF (BRAF Antibody Abstract)
Background BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome), and a wide range of cancers such as lung cancer, non-Hodgkins lymphoma, and colorectal cancer.
Synonyms: B-Raf proto-oncogene serine/threonine-protein kinase, p94, v-Raf murine sarcoma viral oncogene homolog B1
Molecular Weight 84437 DA
Gene ID 673
UniProt P15056
Pathways MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy

Application Details

Product Details anti-BRAF Antibody Target Details BRAF Handling References for anti-BRAF antibody (ABIN389897) Images back to top
Application Notes DB = 1:500
Restrictions For Research Use only

Handling

Product Details anti-BRAF Antibody Target Details BRAF Application Details References for anti-BRAF antibody (ABIN389897) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-BRAF antibody (ABIN389897)

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling Images back to top
Product cited in:

Duan, Cobb: "Calcineurin increases glucose activation of ERK1/2 by reversing negative feedback." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 51, pp. 22314-9, 2010

Background publications

Frattini, Ferrario, Bressan, Balestra, De Cecco, Mondellini, Bongarzone, Collini, Gariboldi, Pilotti, Pierotti, Greco: "Alternative mutations of BRAF, RET and NTRK1 are associated with similar but distinct gene expression patterns in papillary thyroid cancer." in: Oncogene, Vol. 23, Issue 44, pp. 7436-40, 2004

Tsavachidou, Coleman, Athanasiadis, Li, Licht, Olson, Weber: "SPRY2 is an inhibitor of the ras/extracellular signal-regulated kinase pathway in melanocytes and melanoma cells with wild-type BRAF but not with the V599E mutant." in: Cancer research, Vol. 64, Issue 16, pp. 5556-9, 2004

Images

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling References for anti-BRAF antibody (ABIN389897) back to top
Supplier Images
Dot Blot (DB) image for anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) (pThr400) antibody (ABIN389897) Dot blot analysis of anti-BRAF Phospho-specific Pab (ABIN389897) on nitrocellulose me...