anti-Human BRAF antibody for Immunofluorescence

Recommended BRAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
  • B-RAF1
  • BRAF1
  • NS7
  • RAFB1
  • C-RMIL
  • RMIL
  • B-Raf
  • braf1
  • rafb1
  • b-raf1
  • sierrabraf
  • BRAF
  • 9930012E13Rik
  • AA120551
  • AA387315
  • AA473386
  • B-raf
  • Braf-2
  • Braf2
  • C230098H17
  • C87398
  • D6Ertd631e
  • B-Raf proto-oncogene, serine/threonine kinase
  • B-Raf proto-oncogene, serine/threonine kinase S homeolog
  • Braf transforming gene
  • BRAF
  • Braf
  • braf.S
  • braf
Epitope
AA 424-453
36
24
24
21
20
20
20
18
18
16
15
13
10
9
9
6
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
397
84
68
13
9
3
3
2
1
1
1
1
Host
Rabbit
300
94
3
1
Clonality
Polyclonal
Conjugate
This BRAF antibody is un-conjugated
20
19
19
16
16
16
3
3
3
3
3
3
3
3
3
3
3
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
264
207
176
73
72
45
39
17
11
5
4
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN3029542
$ 411.13
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.949394 ABIN3032637 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 424-453 Log in to see Polyclonal 0
11.949394 ABIN2716495 FACS IF WB Mouse IgG1 Log in to see 1D2 0
11.949394 ABIN2716488 IF IHC WB Mouse IgG2b Log in to see 3E8 0
11.949394 ABIN513808 IF ELISA WB Mouse IgG2a kappa AA 138-231, partial Log in to see 1H7 0
11.949394 ABIN513802 IF ELISA WB Mouse IgG2a kappa AA 138-231, partial Log in to see 2F7 0
11.949394 ABIN1105489 EIA IF IHC (p) WB Mouse IgG1 Log in to see 1H12 2
1 ABIN1496951 FACS IF IHC IHC (p) WB Mouse IgG1 Log in to see 5A9 0
1 ABIN1496953 FACS IF WB Mouse IgG1 Log in to see 1D2 0
1 ABIN1496952 FACS IF IHC IHC (p) WB Mouse IgG1 Log in to see 1D4 0
1 ABIN1496954 IF IHC IHC (p) WB Mouse IgG2a Log in to see 3A7 0
1 ABIN2879057 ELISA IF IHC IHC (p) WB Rabbit IgG AA 171-220 Log in to see Polyclonal 0
1 ABIN218330 IF IHC IHC (p) Rabbit IgG Ser601, pThr598 Log in to see Polyclonal 0
1 ABIN462545 ELISA IF IHC IHC (p) WB Mouse IgG1 Log in to see 1H12 0
1 ABIN1496955 IF IHC IHC (p) WB Mouse IgG2b Log in to see 3E8 0
1 ABIN302969 IF IHC (p) Rabbit IgG Ser601, pThr598 Log in to see Polyclonal 0
1 ABIN2716487 IF IHC WB Mouse IgG2a Log in to see 3A7 0
1 ABIN2716497 FACS IF IHC WB Mouse IgG1 Log in to see 1D4 0
1 ABIN2716496 FACS IF IHC WB Mouse IgG1 Log in to see 1E2 0
1 ABIN2716484 FACS IF IHC WB Mouse IgG1 Log in to see 5A9 0
1 ABIN2469737 IF IHC Rabbit IgG Ser601, pThr598 Log in to see Polyclonal 0

General

Antigen V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
Epitope AA 424-453
(36), (24), (24), (21), (20), (20), (20), (18), (18), (16), (15), (13), (10), (9), (9), (6), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(397), (84), (68), (13), (9), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(300), (94), (3), (1)
Clonality
Polyclonal
Conjugate This BRAF antibody is un-conjugated
(20), (19), (19), (16), (16), (16), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(264), (207), (176), (73), (72), (45), (39), (17), (11), (5), (4), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-BRAF Antibody

Target Details BRAF Application Details Handling Images
Cross-Reactivity (Details) Expected species reactivity: Mouse, Chicken
Purification Antigen affinity purified
Immunogen A portion of amino acids 424-453 from the human protein was used as the immunogen for this BRAF antibody.
Isotype Ig Fraction

Target Details BRAF

Product Details anti-BRAF Antibody Application Details Handling Images back to top
Antigen
Alternative Name BRAF (BRAF Antibody Abstract)
Background BRAF is a protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. [UniProt]
UniProt P15056
Pathways MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy

Application Details

Product Details anti-BRAF Antibody Target Details BRAF Handling Images back to top
Application Notes The stated application concentrations are suggested starting amounts.Titration of the BRAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Restrictions For Research Use only

Handling

Product Details anti-BRAF Antibody Target Details BRAF Application Details Images back to top
Format Liquid
Buffer In 1X PBS,  pH 7.4, with 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at 4°C for up to one month. For long term, aliquot the BRAF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Images

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) (AA 424-453) antibody (ABIN3029542) BRAF antibody flow cytometric analysis of HeLa cells (right histogram) compared to a ...
Immunofluorescence (IF) image for anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) (AA 424-453) antibody (ABIN3029542) Confocal immunofluorescent analysis of BRAF antibody with HeLa cells followed by Alex...
Immunohistochemistry (IHC) image for anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) (AA 424-453) antibody (ABIN3029542) IHC analysis of FFPE human brain tissue stained with BRAF antibody
Western Blotting (WB) image for anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) (AA 424-453) antibody (ABIN3029542) Western blot analysis of BRAF antibody and 293 cell lysate (2 ug/lane) either nontran...