anti-Human BRAF antibody for Western Blotting

Recommended BRAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
  • B-RAF1
  • BRAF1
  • NS7
  • RAFB1
  • C-RMIL
  • RMIL
  • B-Raf
  • braf1
  • rafb1
  • b-raf1
  • sierrabraf
  • BRAF
  • 9930012E13Rik
  • AA120551
  • AA387315
  • AA473386
  • B-raf
  • Braf-2
  • Braf2
  • C230098H17
  • C87398
  • D6Ertd631e
  • braf
  • fc48g01
  • wu:fc48g01
  • B-Raf proto-oncogene, serine/threonine kinase
  • B-Raf proto-oncogene, serine/threonine kinase S homeolog
  • Braf transforming gene
  • si:dkey-222f8.3
  • BRAF
  • Braf
  • braf.S
  • braf
  • si:dkey-222f8.3
Epitope
AA 379-408, Thr400
36
33
29
24
22
21
21
18
16
13
11
10
9
9
8
6
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
406
84
68
13
9
3
3
2
1
1
1
1
Host
Rabbit
309
95
3
Clonality
Polyclonal
Conjugate
This BRAF antibody is un-conjugated
20
20
20
17
17
17
3
3
3
3
3
3
3
3
3
3
3
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
272
220
150
82
69
47
39
25
11
7
5
4
1
1
1
1
Options
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Catalog No. ABIN392248
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2286417 ABIN3043761 WB Rabbit IgG AA 38-230 Log in to see Polyclonal 0
3.2286417 ABIN1105488 EIA IHC (p) WB Mouse IgG1 Log in to see 1H12F1 5
3.2286417 ABIN513800 PLA ELISA WB Mouse IgG1 kappa AA 346-445, partial Log in to see 3D2 1
3.2286417 ABIN3043016 WB Rabbit IgG AA 344-362, Middle Region Log in to see Polyclonal 0
3.2286417 ABIN1724658 IHC ELISA WB Mouse IgG1 Log in to see 1H12F1 5
3.2286417 ABIN1871340 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.2286417 ABIN1881118 FACS WB Rabbit Log in to see Polyclonal 6
3.2286417 ABIN968991 ICC IHC ELISA WB Mouse IgG1 Log in to see 1H12 2
3.2286417 ABIN2736363 WB Rabbit IgG Log in to see Polyclonal 0
3.2286417 ABIN931447 IHC ELISA WB Mouse IgG1 Log in to see 1H12F1-1H12G10-1F12F11C9 0
3.2286417 ABIN499008 IHC (p) WB Rabbit Log in to see Polyclonal 0
3.2286417 ABIN1870020 WB Rabbit IgG pSer445 Log in to see Polyclonal 0
3.2286417 ABIN1585648 ELISA IHC WB Rabbit N-Term Log in to see Polyclonal 0
3.2286417 ABIN2452447 IF IHC WB Mouse IgG2b Log in to see 3E8 0
3.2286417 ABIN2452815 FACS IF WB Mouse IgG1 Log in to see 1D2 0
3.2286417 ABIN2452280 WB Mouse IgG2a Log in to see 4B2 0
3.2286417 ABIN2452282 WB Mouse IgG1 Log in to see 3C3 0
3.2286417 ABIN2561461 WB Rabbit IgG pSer728 Log in to see Polyclonal 0
3.2286417 ABIN513799 ELISA WB Mouse IgM kappa AA 346-445, partial Log in to see 3C6 0
3.2286417 ABIN560094 ELISA WB Mouse IgG1 kappa AA 346-445, partial Log in to see 3C6 0

General

Antigen V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
Epitope AA 379-408, Thr400
(36), (33), (29), (24), (22), (21), (21), (18), (16), (13), (11), (10), (9), (9), (8), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(406), (84), (68), (13), (9), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(309), (95), (3)
Clonality (Clone)
Polyclonal   ( )
Conjugate This BRAF antibody is un-conjugated
(20), (20), (20), (17), (17), (17), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(272), (220), (150), (82), (69), (47), (39), (25), (11), (7), (5), (4), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-BRAF Antibody

Target Details BRAF Application Details Handling References for anti-BRAF antibody (ABIN392248) Images
Specificity This BRAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T400 of human BRAF.
Predicted Reactivity Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This BRAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 379-408 AA from human BRAF.
Clone RB13721
Isotype Ig Fraction

Target Details BRAF

Product Details anti-BRAF Antibody Application Details Handling References for anti-BRAF antibody (ABIN392248) Images back to top
Antigen
Alternative Name BRAF (BRAF Antibody Abstract)
Background BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.
Synonyms: B-Raf proto-oncogene serine/threonine-protein kinase, p94, v-Raf murine sarcoma viral oncogene homolog B1
Molecular Weight 84490 DA
Gene ID 673
UniProt P15056
Pathways MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy

Application Details

Product Details anti-BRAF Antibody Target Details BRAF Handling References for anti-BRAF antibody (ABIN392248) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-BRAF Antibody Target Details BRAF Application Details References for anti-BRAF antibody (ABIN392248) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-BRAF antibody (ABIN392248)

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling Images back to top
Background publications

Hingorani, Jacobetz, Robertson, Herlyn, Tuveson: "Suppression of BRAF(V599E) in human melanoma abrogates transformation." in: Cancer research, Vol. 63, Issue 17, pp. 5198-202, 2003

Davies, Bignell, Cox, Stephens, Edkins, Clegg, Teague, Woffendin, Garnett, Bottomley, Davis, Dicks, Ewing, Floyd, Gray, Hall, Hawes, Hughes, Kosmidou, Menzies, Mould, Parker, Stevens, Watt, Hooper et al.: "Mutations of the BRAF gene in human cancer. ..." in: Nature, Vol. 417, Issue 6892, pp. 949-54, 2002

Naoki, Chen, Richards, Sugarbaker, Meyerson: "Missense mutations of the BRAF gene in human lung adenocarcinoma." in: Cancer research, Vol. 62, Issue 23, pp. 7001-3, 2002

Images

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling References for anti-BRAF antibody (ABIN392248) back to top
Supplier Images
Western Blotting (WB) image for anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) (AA 379-408), (Thr400) antibody (ABIN392248) Western blot analysis of BRAF (arrow) using rabbit polyclonal BRAF Antibody (T400) (A...
Immunohistochemistry (IHC) image for anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) (AA 379-408), (Thr400) antibody (ABIN392248) Formalin-fixed and paraffin-embedded human brain tissue reacted with BRAF-pT400, whic...