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|Antigen||V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This BRAF antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-BRAF AntibodyTarget Details BRAF Application Details Handling Images
|Specificity||This antibody detects endogenous levels of Raf-B protein. (region surrounding Lys439)|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, Mouse.|
|Purity||> 95 % by SDS-PAGE|
Target Details BRAFProduct Details anti-BRAF Antibody Application Details Handling Images back to top
|Background||Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted with highest levels in the cerebrum and testis.Synonyms: B-Raf proto-oncogene serine/threonine-protein kinase, BRAF, BRAF1, RAFB1, p94, v-Raf murine sarcoma viral oncogene homolog B1|
|Molecular Weight||approx. 86 kDa|
|Pathways||MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy|
Application DetailsProduct Details anti-BRAF Antibody Target Details BRAF Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-BRAF Antibody Target Details BRAF Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-BRAF Antibody Target Details BRAF Application Details Handling back to top