anti-Mouse (Murine) Calnexin antibody for Enzyme Immunoassay

Recommended Calnexin Antibody (supplied by: Log in to see )

Antigen
Calnexin (CANX) Antibodies
  • CNX
  • IP90
  • P90
  • 1110069N15Rik
  • AI988026
  • Cnx
  • D11Ertd153e
  • canx
  • clgn
  • clnx
  • cnx
  • PP90
  • zgc:63524
  • wu:fe06b12
  • CANX
  • calnexin
  • CalNeXin
  • calnexin L homeolog
  • CANX
  • Canx
  • cnx-1
  • canx.L
  • canx
  • LOC100283947
Epitope
Internal Region
137
61
19
14
13
13
12
7
5
5
4
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
471
298
286
184
177
171
169
165
165
159
159
158
104
95
58
54
54
16
13
7
6
6
4
4
3
2
2
1
1
1
1
1
Host
Goat
310
152
30
Clonality
Polyclonal
Conjugate
This Calnexin antibody is un-conjugated
20
18
17
13
13
12
9
8
8
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
6
5
5
5
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
451
338
237
190
160
159
129
87
67
21
14
9
9
8
3
2
1
1
1
1
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Catalog No. ABIN571025
$ 429.62
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN2561540 EIA IHC ELISA WB Goat IgG Internal Region Log in to see Polyclonal 0
1 ABIN5551877 EIA IHC (p) WB Goat Internal Region Log in to see Polyclonal 0
1 ABIN4950397 ELISA EIA IHC WB Goat Ig Fraction Log in to see Polyclonal 0

General

Antigen Calnexin (CANX) Antibodies
Epitope Internal Region
(137), (61), (19), (14), (13), (13), (12), (7), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(471), (298), (286), (184), (177), (171), (169), (165), (165), (159), (159), (158), (104), (95), (58), (54), (54), (16), (13), (7), (6), (6), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Host Goat
(310), (152), (30)
Clonality
Polyclonal
Conjugate This Calnexin antibody is un-conjugated
(20), (18), (17), (13), (13), (12), (9), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (5), (5), (5), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(451), (338), (237), (190), (160), (159), (129), (87), (67), (21), (14), (9), (9), (8), (3), (2), (1), (1), (1), (1)
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Product Details anti-Calnexin Antibody

Target Details Calnexin Application Details Handling Images
Purpose Calnexin
Blocking Peptide Blocking peptide for this product available: ABIN2815312
Sequence SKTPELNLDQ FHDKT
Specificity Reported variants represent identical protein (NP_001019820.1, NP_001737.1).
Predicted Reactivity Human, Mouse, Rat, Dog, Pig, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Immunogen Peptide with sequence C-SKTPELNLDQFHDKT, from the internal region (near N Terminus) of the protein sequence according to NP_001737.1.
Plasmids, Primers & others

Target Details Calnexin

Product Details anti-Calnexin Antibody Application Details Handling Images back to top
Antigen
Alternative Name CANX (CANX Antibody Abstract)
Background CANX, calnexin, CNX, FLJ26570, IP90, P90, major histocompatibility complex class I antigen-binding protein p88
Gene ID 821
NCBI Accession NP_001737
Pathways MAPK Signaling, Thyroid Hormone Synthesis

Application Details

Product Details anti-Calnexin Antibody Target Details Calnexin Handling Images back to top
Application Notes

DS WB Results: Approx. 100 kDa band observed in Mouse Heart, Human Brain (Cerebellum), NIH3T3 and HeLa lysates (calculated MW of 67.6 kDa according to Human NP_001737.1 and 67.2 kDa according to Mouse NP_001103969.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Oliver et al., J Biol Chem. 1996 Jun 7,271(23):13691-6., PMID: 8662691). Recommended concentration: 0.3-1 μg/mL.

DS IHC Results: In paraffin embedded Human Kidney shows textured staining in the cytoplasm of DCT cells. Recommended concentration: 3-6 μg/mL. Paraffin embedded Human Testis and Uterus. Recommended concentration: 3.75 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:32000.

Comment

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 5μg/ml.

Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant Calnexin captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml

Restrictions For Research Use only

Handling

Product Details anti-Calnexin Antibody Target Details Calnexin Application Details Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

Images

Product Details anti-Calnexin Antibody Target Details Calnexin Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Calnexin (CANX) (Internal Region) antibody (ABIN571025) Antibody (0.3µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Pri...
Enzyme Immunoassay (EIA) image for anti-Calnexin (CANX) (Internal Region) antibody (ABIN571025) Antibody (1.5ug/ml) as the reporter with EB002024 as the capture rabbit antibody (2.5...