anti-Mouse (Murine) Calnexin antibody for Immunofluorescence

Recommended Calnexin Antibody (supplied by: Log in to see )

Antigen
Calnexin (CANX) Antibodies
  • CNX
  • IP90
  • P90
  • 1110069N15Rik
  • AI988026
  • Cnx
  • D11Ertd153e
  • canx
  • clgn
  • clnx
  • cnx
  • PP90
  • zgc:63524
  • wu:fe06b12
  • CANX
  • calnexin
  • CalNeXin
  • calnexin L homeolog
  • CANX
  • Canx
  • cnx-1
  • canx.L
  • canx
  • LOC100283947
Epitope
C-Term, AA 550-591
135
63
20
14
13
12
12
7
5
5
5
4
4
4
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Hamster, Human, Mouse (Murine), Rat (Rattus)
473
302
289
181
175
173
167
163
163
158
157
155
104
95
58
54
54
16
11
5
5
4
4
4
3
2
1
1
1
1
1
Host
Rabbit
310
154
29
Clonality
Polyclonal
Conjugate
This Calnexin antibody is un-conjugated
20
18
17
15
14
13
9
8
8
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
6
5
5
5
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
451
340
238
194
161
160
128
89
67
21
13
9
9
6
2
2
1
1
1
1
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Catalog No. ABIN151474
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.3485155 ABIN151464 ICC IF IHC IHC (p) IP SimWes WB Rabbit Internal Region, AA 25-100 Log in to see Polyclonal 9
11.3485155 ABIN2451929 IF IP IHC WB Rabbit C-Term Log in to see Polyclonal 0
11.3485155 ABIN152677 ICC IF IHC (p) IP IHC WB Mouse IgG1 Log in to see AF18 4
11.3485155 ABIN2855124 ICC IF IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal 0
11.3485155 ABIN2704566 IC IF IP IHC WB Rabbit C-Term, pSer583 Log in to see Polyclonal 0
11.3485155 ABIN2704567 IC IF IP IHC WB Rabbit C-Term Log in to see Polyclonal 0
11.3485155 ABIN1532324 IF IHC ELISA WB Rabbit IgG AA 543-592 Log in to see Polyclonal 0
11.3485155 ABIN1531305 IF IHC ELISA WB Rabbit IgG AA 543-592, pSer583 Log in to see Polyclonal 0
11.3485155 ABIN1440007 IF IHC (fro) IHC (p) WB Goat IgG C-Term Log in to see Polyclonal 1
11.3485155 ABIN1440005 IF IHC (fro) IHC (p) WB Goat IgG C-Term Log in to see Polyclonal 0
1 ABIN2481636 ICC FACS IF IP IHC WB Atto 680 Rabbit C-Term Log in to see Polyclonal 7
1 ABIN2481642 ICC FACS IF IP IHC WB HRP Rabbit C-Term Log in to see Polyclonal 7
1 ABIN2481646 ICC FACS IF IP IHC WB Streptavidin Rabbit C-Term Log in to see Polyclonal 7
1 ABIN2481645 ICC FACS IF IP IHC WB PE Rabbit C-Term Log in to see Polyclonal 7
1 ABIN2481630 ICC FACS IF IP IHC WB Atto 390 Rabbit C-Term Log in to see Polyclonal 7
1 ABIN2481633 ICC FACS IF IP IHC WB Atto 594 Rabbit C-Term Log in to see Polyclonal 7
1 ABIN2481634 ICC FACS IF IP IHC WB Atto 633 Rabbit C-Term Log in to see Polyclonal 7
1 ABIN2481635 ICC FACS IF IP IHC WB Atto 655 Rabbit C-Term Log in to see Polyclonal 7
1 ABIN2481639 ICC FACS IF IP IHC WB APC Rabbit C-Term Log in to see Polyclonal 7
1 ABIN2481640 ICC FACS IF IP IHC WB Biotin Rabbit C-Term Log in to see Polyclonal 7

General

Antigen Calnexin (CANX) Antibodies
Epitope C-Term, AA 550-591
(135), (63), (20), (14), (13), (12), (12), (7), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Hamster, Human, Mouse (Murine), Rat (Rattus)
(473), (302), (289), (181), (175), (173), (167), (163), (163), (158), (157), (155), (104), (95), (58), (54), (54), (16), (11), (5), (5), (4), (4), (4), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(310), (154), (29)
Clonality
Polyclonal
Conjugate This Calnexin antibody is un-conjugated
(20), (18), (17), (15), (14), (13), (9), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (5), (5), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
(451), (340), (238), (194), (161), (160), (128), (89), (67), (21), (13), (9), (9), (6), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-Calnexin Antibody

Target Details Calnexin Application Details Handling References for anti-Calnexin antibody (ABIN151474) Images
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to a C-terminal portion of the rat Calnexin protein (between residues 550-591) [UniProt P35565]
Plasmids, Primers & others

Target Details Calnexin

Product Details anti-Calnexin Antibody Application Details Handling References for anti-Calnexin antibody (ABIN151474) Images back to top
Antigen
Alternative Name Calnexin (CANX Antibody Abstract)
Background Gene Symbol: CANX
Molecular Weight Theoretical MW: 97 kDa
Gene ID 821
UniProt P35565
Pathways MAPK Signaling, Thyroid Hormone Synthesis

Application Details

Product Details anti-Calnexin Antibody Target Details Calnexin Handling References for anti-Calnexin antibody (ABIN151474) Images back to top
Application Notes Western Blot 1:1000, Simple Western 1:50, Flow Cytometry, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:100, Immunohistochemistry-Paraffin 1:100This Calnexin antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western Blot, and Immunohistochemistry-paraffin embedded sections. In ICC/IF, endoplasmic reticulum staining was observed in HeLa cells. In Western Blot, a band is seen approx. 90 kDa representing Calnexin. In IHC-P, staining was observed in the endoplasmic reticulum of mouse bladder tisSue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Calnexin antibody Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room
Restrictions For Research Use only

Handling

Product Details anti-Calnexin Antibody Target Details Calnexin Application Details References for anti-Calnexin antibody (ABIN151474) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS and 30 % Glycerol
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-Calnexin antibody (ABIN151474)

Product Details anti-Calnexin Antibody Target Details Calnexin Application Details Handling Images back to top
Product cited in:

Ye, Zhang, Ancrum, Manevich, Townsend, Tew: "Glutathione S-Transferase P-Mediated Protein S-Glutathionylation of Resident Endoplasmic Reticulum Proteins Influences Sensitivity to Drug-Induced Unfolded Protein Response." in: Antioxidants & redox signaling, 2016

Kang, Tang, Schapiro, Loux, Livesey, Billiar, Wang, Van Houten, Lotze, Zeh: "The HMGB1/RAGE inflammatory pathway promotes pancreatic tumor growth by regulating mitochondrial bioenergetics." in: Oncogene, Vol. 33, Issue 5, pp. 567-77, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Xu, Xu, Brown, Rossi, Hurd, Inturrisi, Pasternak, Pan: "Stabilization of the μ-opioid receptor by truncated single transmembrane splice variants through a chaperone-like action." in: The Journal of biological chemistry, Vol. 288, Issue 29, pp. 21211-27, 2013 (Sample species: Hamster). Further details: Western Blotting

Ghosh, Vo, Twiss, Kretz, Jozwiak, Montgomery, Shavit: "Characterization of zebrafish von Willebrand factor reveals conservation of domain structure, multimerization, and intracellular storage." in: Advances in hematology, Vol. 2012, pp. 214209, 2012 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Images

Product Details anti-Calnexin Antibody Target Details Calnexin Application Details Handling References for anti-Calnexin antibody (ABIN151474) back to top
Supplier Images
Western Blotting (WB) image for anti-Calnexin (CANX) (AA 550-591), (C-Term) antibody (ABIN151474) anti-Calnexin (CANX) (AA 550-591), (C-Term) antibody
Simple Western (SimWes) image for anti-Calnexin (CANX) (AA 550-591), (C-Term) antibody (ABIN151474) Simple Western: Calnexin Antibody [ABIN151474] - Simple Western lane view shows a spe...
Flow Cytometry (FACS) image for anti-Calnexin (CANX) (AA 550-591), (C-Term) antibody (ABIN151474) Flow Cytometry: Calnexin Antibody [ABIN151474] - Analysis of Alexa Fluor (R) 488 conj...
Western Blotting (WB) image for anti-Calnexin (CANX) (AA 550-591), (C-Term) antibody (ABIN151474) Western Blot: Calnexin Antibody [ABIN151474] - WB analysis of Calnexin in HeLa whole ...
Flow Cytometry (FACS) image for anti-Calnexin (CANX) (AA 550-591), (C-Term) antibody (ABIN151474) Flow Cytometry: Calnexin Antibody [ABIN151474] - Analysis of Alexa Fluor (R) 488 conj...
Immunohistochemistry (IHC) image for anti-Calnexin (CANX) (AA 550-591), (C-Term) antibody (ABIN151474) Immunohistochemistry: Calnexin Antibody [ABIN151474] - IHC analysis of Calnexin in mo...
Immunofluorescence (IF) image for anti-Calnexin (CANX) (AA 550-591), (C-Term) antibody (ABIN151474) Immunocytochemistry/Immunofluorescence: Calnexin Antibody [ABIN151474] - CANX antibod...