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|Antigen||Calnexin (CANX) Antibodies|
|Epitope||Internal Region, AA 25-100 Alternatives|
|Reactivity||Bird (Avian), Chicken, Cow (Bovine), Fruit Fly (Drosophila melanogaster), Guinea Pig, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis, Zebrafish (Danio rerio) Alternatives|
|Conjugate||This Calnexin antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
|9 references available|
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Product Details anti-Calnexin AntibodyTarget Details Calnexin Application Details Handling References for anti-Calnexin antibody (ABIN151464) Images
|Characteristics||Calnexin is a chaperone protein that assists in protein folding and quality control. Calnexin retains unassembled or unfolded N-linked glycoproteins in the endoplasmic reticulum and ensures that only properly assembled and folded proteins proceed down the secretory pathway. Calnexin antibodies can be used as endoplasmic reticulum markers (ER markers) in immunostaining experiments.|
|Purification||Immunogen affinity purified|
|Immunogen||A synthetic peptide made to an internal region of the canine Calnexin protein (within residues 25-100). [Swiss-Prot P24643]|
|Plasmids, Primers & others|
Target Details CalnexinProduct Details anti-Calnexin Antibody Application Details Handling References for anti-Calnexin antibody (ABIN151464) Images back to top
|Alternative Name||Calnexin (CANX Antibody Abstract)|
|Background||Gene Symbol: CANX|
|Molecular Weight||Theoretical MW: 97 kDa|
|Pathways||MAPK Signaling, Thyroid Hormone Synthesis|
Application DetailsProduct Details anti-Calnexin Antibody Target Details Calnexin Handling References for anti-Calnexin antibody (ABIN151464) Images back to top
|Application Notes||Western Blot 2 μg/mL, Simple Western 1:25, Immunohistochemistry 1:40, Immunocytochemistry/Immunofluorescence 1:50, Immunoprecipitation 1:100, Immunohistochemistry-Paraffin 1:40This Calnexin antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, Immunoprecipitation and Western Blot. In Western blot a band is observed approx. 97 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Western Blot Protocol Specific for CANX antibody
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry-Paraffin Embedded Sections Antigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Calnexin Antibody Target Details Calnexin Application Details References for anti-Calnexin antibody (ABIN151464) Images back to top
Tris-Glycine and 0.15M NaCl
Buffer contains: 0.5 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage||4 °C,-20 °C|
|Storage Comment||Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
References for anti-Calnexin antibody (ABIN151464)Product Details anti-Calnexin Antibody Target Details Calnexin Application Details Handling Images back to top
|Product cited in:||
Li, Subrahmanyan, Smith, Yu, Zaidi, Choi, Mane, Nelson-Williams, Bahjati, Kazemi, Hashemi, Fathzadeh, Narayanan, Tian, Montazeri, Mani, Begleiter, Coon, Lynch, Olson, Zhao, Ruland, Lifton, Mani: "Mutations in the Histone Modifier PRDM6 Are Associated with Isolated Nonsyndromic Patent Ductus Arteriosus." in: American journal of human genetics, Vol. 98, Issue 6, pp. 1082-91, 2016 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence
Xu, Li, Zalzala, Xu, Gonzalez, Adorini, Lee, Yin, Zhang: "Farnesoid X receptor activation increases reverse cholesterol transport by modulating bile acid composition and cholesterol absorption in mice." in: Hepatology (Baltimore, Md.), Vol. 64, Issue 4, pp. 1072-85, 2016
Huynh, VonMoss, Smith, Rahman, Felemban, Zuo, Rody, McHugh, Holliday: "Characterization of Regulatory Extracellular Vesicles from Osteoclasts." in: Journal of dental research, Vol. 95, Issue 6, pp. 673-9, 2016 Method employed by authors: Immunoprecipitation (IP)
Smagris, Gilyard, BasuRay, Cohen, Hobbs: "Inactivation of Tm6sf2, a Gene Defective in Fatty Liver Disease, Impairs Lipidation but Not Secretion of Very Low Density Lipoproteins." in: The Journal of biological chemistry, Vol. 291, Issue 20, pp. 10659-76, 2016
Riemersma, Sandrock, Boltje, Büll, Heise, Ashikov, Adema, van Bokhoven, Lefeber: "Disease mutations in CMP-sialic acid transporter SLC35A1 result in abnormal α-dystroglycan O-mannosylation, independent from sialic acid." in: Human molecular genetics, Vol. 24, Issue 8, pp. 2241-6, 2015 (Sample species: Human). Further details: Western Blotting
McFarlane, Cantoria, Linden, January, Liang, Engelking: "Scap is required for sterol synthesis and crypt growth in intestinal mucosa." in: Journal of lipid research, Vol. 56, Issue 8, pp. 1560-71, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting
Yuen, Webb, Chan, Thisse, Thisse, Miller: "Characterization of Ca(2+) signaling in the external yolk syncytial layer during the late blastula and early gastrula periods of zebrafish development." in: Biochimica et biophysica acta, Vol. 1833, Issue 7, pp. 1641-56, 2013 (Sample species: Zebrafish (Danio rerio)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry
Saeki, Sato, Ito, Ikeda, Kanamoto et al.: "Importance of uncharged polar residues and proline in the proximal two-thirds (Pro107-Ser128) of the highly conserved region of mouse ileal Na+-dependent bile acid transporter, Slc10a2, in transport ..." in: BMC physiology, Vol. 13, pp. 4, 2013 (Sample species: Pig (Porcine)). Further details: Western Blotting
Tian, Hoffman, Ten Hagen: "O-glycosylation modulates integrin and FGF signalling by influencing the secretion of basement membrane components." in: Nature communications, Vol. 3, pp. 869, 2012 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence
ImagesProduct Details anti-Calnexin Antibody Target Details Calnexin Application Details Handling References for anti-Calnexin antibody (ABIN151464) back to top