anti-Rat (Rattus) Calnexin antibody for Enzyme Immunoassay

Recommended Calnexin Antibody (supplied by: Log in to see )

Antigen
Calnexin (CANX) Antibodies
  • CNX
  • IP90
  • P90
  • 1110069N15Rik
  • AI988026
  • Cnx
  • D11Ertd153e
  • canx
  • clgn
  • clnx
  • cnx
  • PP90
  • zgc:63524
  • wu:fe06b12
  • CANX
  • calnexin
  • CalNeXin
  • calnexin L homeolog
  • CANX
  • Canx
  • cnx-1
  • canx.L
  • canx
  • LOC100283947
Epitope
Internal Region
136
80
19
14
13
13
12
7
5
5
5
4
4
4
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
487
300
287
181
175
174
166
164
164
158
157
157
104
95
58
54
54
16
12
5
5
4
4
4
4
3
2
1
1
1
1
1
1
Host
Goat
308
171
28
Clonality
Polyclonal
Conjugate
This Calnexin antibody is un-conjugated
21
18
18
15
14
13
10
8
8
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
6
5
5
5
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
465
356
255
194
176
155
129
89
67
38
13
9
8
3
2
2
1
1
1
1
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Catalog No. ABIN5551877
$ 462.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2561540 EIA IHC ELISA WB Goat IgG Internal Region Log in to see Polyclonal 0

General

Antigen Calnexin (CANX) Antibodies
Epitope Internal Region
(136), (80), (19), (14), (13), (13), (12), (7), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(487), (300), (287), (181), (175), (174), (166), (164), (164), (158), (157), (157), (104), (95), (58), (54), (54), (16), (12), (5), (5), (4), (4), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Host Goat
(308), (171), (28)
Clonality
Polyclonal
Conjugate This Calnexin antibody is un-conjugated
(21), (18), (18), (15), (14), (13), (10), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(465), (356), (255), (194), (176), (155), (129), (89), (67), (38), (13), (9), (8), (3), (2), (2), (1), (1), (1), (1)
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Product Details anti-Calnexin Antibody

Target Details Calnexin Application Details Handling Images
Sequence c-SKTPELNLDQ FHDKT
Specificity This antibody recognizes Calnexin.
Cross-Reactivity (Details) Species reactivity (expected):Rat, Canine, Pig, Bovine.
Species reactivity (tested):Human, Mouse.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Immunogen Peptide with sequence from the internal region (near N-Terminus) of the protein sequence . Genename: CANX
Plasmids, Primers & others

Target Details Calnexin

Product Details anti-Calnexin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Calnexin (CANX Antibody Abstract)
Background Calnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatability complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP 78 and GRP 94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids). These features distinguish calnexin from soluble ER chaperones that cannot interact with the transmembrane and cytosolic domains of integral membrane proteins. The amino acid sequence of calnexin is highly conserved among species, and shares regions of high sequence homology with calreticulin.Synonyms: CANX, ER Marker, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90
Gene ID 821
NCBI Accession NP_001737
UniProt P27824
Pathways MAPK Signaling, Thyroid Hormone Synthesis

Application Details

Product Details anti-Calnexin Antibody Target Details Calnexin Handling Images back to top
Application Notes Peptide ELISA: 1/32000. Western blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Calnexin Antibody Target Details Calnexin Application Details Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.