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|Antigen||ELK1, Member of ETS Oncogene Family (ELK1) Antibodies|
|Conjugate||This ELK1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-ELK1 AntibodyTarget Details ELK1 Application Details Handling References for anti-ELK1 antibody (ABIN358791) Images
|Specificity||This antibody reacts to ELK1.|
|Purification||Protein A column, followed by peptide affinity purification|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S383 of human ELK1.|
Target Details ELK1Product Details anti-ELK1 Antibody Application Details Handling References for anti-ELK1 antibody (ABIN358791) Images back to top
|Alternative Name||ELK1 (ELK1 Antibody Abstract)|
|Background||ELK1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein is a nuclear target for the ras-raf-MAPK signaling cascade.Synonyms: ETS domain-containing protein Elk-1, Elk-1|
|Gene ID||2002, 9606|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-ELK1 Antibody Target Details ELK1 Handling References for anti-ELK1 antibody (ABIN358791) Images back to top
ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ELK1 Antibody Target Details ELK1 Application Details References for anti-ELK1 antibody (ABIN358791) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-ELK1 antibody (ABIN358791)Product Details anti-ELK1 Antibody Target Details ELK1 Application Details Handling Images back to top
Salinas, Briançon-Marjollet, Bossis, Lopez, Piechaczyk, Jariel-Encontre, Debant, Hipskind: "SUMOylation regulates nucleo-cytoplasmic shuttling of Elk-1." in: The Journal of cell biology, Vol. 165, Issue 6, pp. 767-73, 2004
Yang, Jaffray, Hay, Sharrocks: "Dynamic interplay of the SUMO and ERK pathways in regulating Elk-1 transcriptional activity." in: Molecular cell, Vol. 12, Issue 1, pp. 63-74, 2003
Bébien, Salinas, Becamel, Richard, Linares, Hipskind: "Immediate-early gene induction by the stresses anisomycin and arsenite in human osteosarcoma cells involves MAPK cascade signaling to Elk-1, CREB and SRF." in: Oncogene, Vol. 22, Issue 12, pp. 1836-47, 2003
Murai, Treisman: "Interaction of serum response factor (SRF) with the Elk-1 B box inhibits RhoA-actin signaling to SRF and potentiates transcriptional activation by Elk-1." in: Molecular and cellular biology, Vol. 22, Issue 20, pp. 7083-92, 2002
Sharrocks: "Complexities in ETS-domain transcription factor function and regulation: lessons from the TCF (ternary complex factor) subfamily. The Colworth Medal Lecture." in: Biochemical Society transactions, Vol. 30, Issue 2, pp. 1-9, 2002
ImagesProduct Details anti-ELK1 Antibody Target Details ELK1 Application Details Handling References for anti-ELK1 antibody (ABIN358791) back to top