anti-Human ERK2 antibody for Immunofluorescence

Recommended ERK2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
  • ERK
  • ERK2
  • ERT1
  • MAPK2
  • P42MAPK
  • PRKM1
  • PRKM2
  • p38
  • p40
  • p41
  • p41mapk
  • 9030612K14Rik
  • AA407128
  • AU018647
  • C78273
  • Erk2
  • Prkm1
  • p42mapk
  • zERK2
  • MAPK1
  • Xp42
  • erk
  • erk2
  • ert1
  • mapk
  • mapk1b
  • mapk2
  • mpk1
  • prkm1
  • prkm2
  • mapk1
  • mapk1a
  • xp42
  • ATMPK1
  • F14N23.9
  • F14N23_9
  • MITOGEN-ACTIVATED PROTEIN KINASE
  • mitogen-activated protein kinase 1
  • mitogen-activated protein kinase 1
  • mitogen activated protein kinase 1
  • mitogen-activated protein kinase 1 L homeolog
  • mitogen-activated protein kinase 1 S homeolog
  • MAPK1
  • Mapk1
  • mapk1
  • mapk1.L
  • mapk1.S
  • PfMAP1
  • MPK1
  • PVX_084965
  • LOC9328617
  • Tsp_01601
Epitope
AA 261-361
51
22
19
13
11
9
7
6
6
5
5
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
372
156
139
57
37
19
14
10
9
9
9
7
5
4
2
2
2
2
1
1
1
1
Host
Mouse
181
175
17
8
3
Clonality
Monoclonal
Conjugate
This ERK2 antibody is un-conjugated
13
11
9
7
6
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
1
Application
ELISA, Immunofluorescence (IF), Western Blotting (WB)
349
163
122
82
78
70
49
25
25
10
7
5
3
3
1
1
1
1
1
1
1
1
1
Options
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Catalog No. ABIN393485
$ 461.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.3485155 ABIN492382 EIA IF WB Mouse IgG2b AA 1-360, N-Term Log in to see AT1A4 0
11.3485155 ABIN4309415 ICC IF IHC IHC (p) SimWes WB Rabbit IgG Log in to see Polyclonal 0
11.3485155 ABIN1873622 IF IHC WB Rabbit IgG N-Term Log in to see Polyclonal 0
11.3485155 ABIN258758 ICC IHC (fro) IF IHC (p) IHC ELISA WB Mouse IgG2a Log in to see 4C11 0
11.3485155 ABIN562405 IF PLA ELISA WB Mouse IgG1 kappa AA 261-360, full length Log in to see 1D1 0
11.3485155 ABIN2720487 IF IP WB Mouse IgG1 Log in to see 7E7 0
11.3485155 ABIN2720483 IF IP WB Mouse IgG2a Log in to see 7C9 0
11.3485155 ABIN1107133 IF IHC (p) WB Mouse IgG2a Log in to see 4C11 2
11.3485155 ABIN3210001 IF IHC ELISA WB Mouse IgG2a Log in to see 4C11 0
11.3485155 ABIN934060 IF ELISA WB Mouse IgG2b kappa Log in to see AT1A4 0
1 ABIN438932 ICC IF IHC IHC (p) IP PLA WB Rabbit AA 310-360 Log in to see Polyclonal 0
1 ABIN1499290 IF IP WB Mouse IgG1 Log in to see 7E7 0
1 ABIN1499289 IF IHC IHC (p) IP WB Mouse IgG1 Log in to see 4C2 0
1 ABIN5950193 FACS ICC IF IHC IHC (p) IP WB Rabbit IgG C-Term Log in to see SZ25-01 0
1 ABIN1872585 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1499288 IF WB Mouse IgG2a Log in to see 7C9 0
1 ABIN3184547 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0
1 ABIN2720486 IF IP IHC WB Mouse IgG1 Log in to see 4C2 0
1 ABIN6254915 ELISA ICC IF IHC WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN5959463 ELISA IF IHC (p) WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
Epitope AA 261-361
(51), (22), (19), (13), (11), (9), (7), (6), (6), (5), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(372), (156), (139), (57), (37), (19), (14), (10), (9), (9), (9), (7), (5), (4), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(181), (175), (17), (8), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ERK2 antibody is un-conjugated
(13), (11), (9), (7), (6), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Application ELISA, Immunofluorescence (IF), Western Blotting (WB)
(349), (163), (122), (82), (78), (70), (49), (25), (25), (10), (7), (5), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-ERK2 Antibody

Target Details ERK2 Application Details Handling References for anti-ERK2 antibody (ABIN393485) Images
Specificity MAPK1 (AAH17832, 261 a.a. ~ 361 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross-Reactivity (Details) Human: ~~ Positive
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen

Antigen Type: Recombinant Protein

Target/Specificity: MAPK1 (AAH17832, 261 a.a. ~ 360 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone 1D1
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details ERK2

Product Details anti-ERK2 Antibody Application Details Handling References for anti-ERK2 antibody (ABIN393485) Images back to top
Antigen
Alternative Name MAPK1 (MAPK1 Antibody Abstract)
Background

Other Names: Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2, MAPK1, ERK2, PRKM1, PRKM2

Gene Description: mitogen-activated protein kinase 1

Gene Summary: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq

Molecular Weight 41390 DA
Gene ID 5594
NCBI Accession NP_002736, NP_620407
UniProt P28482
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals

Application Details

Product Details anti-ERK2 Antibody Target Details ERK2 Handling References for anti-ERK2 antibody (ABIN393485) Images back to top
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.

Restrictions For Research Use only

Handling

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details References for anti-ERK2 antibody (ABIN393485) Images back to top
Buffer Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-ERK2 antibody (ABIN393485)

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling Images back to top
Background publications

Shimada, Miyagawa, Kawashima, Tanaka, Honda, Honda, Tokunaga: "An approach based on a genome-wide association study reveals candidate loci for narcolepsy." in: Human genetics, Vol. 128, Issue 4, pp. 433-41, 2010

Lujan, Hakim, Moyano, Nadal, Caballero, Diaz, Valera, Carrera, Cardesa, Alos: "Activation of the EGFR/ERK pathway in high-grade mucoepidermoid carcinomas of the salivary glands." in: British journal of cancer, Vol. 103, Issue 4, pp. 510-6, 2010

Johnatty, Beesley, Chen, Macgregor, Duffy, Spurdle, deFazio, Gava, Webb, Rossing, Doherty, Goodman, Lurie, Thompson, Wilkens, Ness, Moysich, Chang-Claude, Wang-Gohrke, Cramer, Terry, Hankinson, Tworoger, Garcia-Closas, Yang, Lissowska, Chanock, Pharoah, S: "Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot"." in: PLoS genetics, Vol. 6, Issue 7, pp. e1001016, 2010

Bailey, Xie, Do, Montpetit, Diaz, Mohan, Keavney, Yusuf, Gerstein, Engert, Anand: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010

Kang, Banfield: "Pseudorabies virus tegument protein Us2 recruits the mitogen-activated protein kinase extracellular-regulated kinase (ERK) to membranes through interaction with the ERK common docking domain." in: Journal of virology, Vol. 84, Issue 17, pp. 8398-408, 2010

Images

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling References for anti-ERK2 antibody (ABIN393485) back to top
Supplier Images
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 261-361) antibody (ABIN393485) Immunofluorescence of monoclonal antibody to MAPK1 on HeLa cell (antibody concentrati...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 261-361) antibody (ABIN393485) anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 261-361) antibody (Image 2)
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 261-361) antibody (ABIN393485) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 261-361) antibody (ABIN393485) MAPK1 monoclonal antibody (M01), clone 1D1 Western Blot analysis of MAPK1 expression ...
Immunofluorescence (IF) image for anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 261-361) antibody (ABIN393485) Immunofluorescence of monoclonal antibody to MAPK1 on HeLa cell. [antibody concentrat...
ELISA image for anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 261-361) antibody (ABIN393485) Detection limit for recombinant GST tagged MAPK1 is approximately 0.03ng/ml as a capt...
 image for anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 261-361) antibody (ABIN393485) Proximity Ligation Analysis of protein-protein interactions between TP53 and MAPK1. H...
 image for anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 261-361) antibody (ABIN393485) Proximity Ligation Analysis of protein-protein interactions between MAPK3 and MAPK1 H...