anti-Human ERK2 antibody for Western Blotting

Recommended ERK2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
  • ERK
  • ERK2
  • ERT1
  • MAPK2
  • P42MAPK
  • PRKM1
  • PRKM2
  • p38
  • p40
  • p41
  • p41mapk
  • 9030612K14Rik
  • AA407128
  • AU018647
  • C78273
  • Erk2
  • Prkm1
  • p42mapk
  • zERK2
  • MAPK1
  • Xp42
  • erk
  • erk2
  • ert1
  • mapk
  • mapk1b
  • mapk2
  • mpk1
  • prkm1
  • prkm2
  • mapk1
  • mapk1a
  • xp42
  • ATMPK1
  • F14N23.9
  • F14N23_9
  • MITOGEN-ACTIVATED PROTEIN KINASE
  • mitogen-activated protein kinase 1
  • mitogen-activated protein kinase 1
  • mitogen activated protein kinase 1
  • mitogen-activated protein kinase 1 L homeolog
  • mitogen-activated protein kinase 1 S homeolog
  • MAPK1
  • Mapk1
  • mapk1
  • mapk1.L
  • mapk1.S
  • PfMAP1
  • MPK1
  • PVX_084965
  • LOC9328617
  • Tsp_01601
Epitope
AA 324-335, C-Term
48
22
18
15
11
10
7
6
6
5
5
4
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
326
135
134
39
37
19
14
10
9
9
9
7
5
4
2
2
2
2
1
1
1
1
Host
Rabbit
176
135
16
7
3
Clonality
Polyclonal
Conjugate
This ERK2 antibody is un-conjugated
11
9
7
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
304
140
115
69
60
57
27
26
10
7
7
5
3
3
1
1
1
1
1
1
1
1
Options
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Catalog No. ABIN5518657
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0813878 ABIN2787871 WB Rabbit Middle Region Log in to see Polyclonal 0
3.0813878 ABIN2787870 WB Rabbit C-Term Log in to see Polyclonal 0
3.0813878 ABIN3042634 WB Rabbit IgG AA 348-360, C-Term Log in to see Polyclonal 2
3.0813878 ABIN1882240 WB Mouse IgG1, kappa AA 154-183 Log in to see 814CT5-4-2 5
3.0813878 ABIN2788693 WB Rabbit C-Term Log in to see Polyclonal 0
3.0813878 ABIN393485 ELISA IF WB Mouse IgG1 kappa AA 261-361 Log in to see 1D1 5
3.0813878 ABIN2706125 WB Rabbit Center Log in to see Polyclonal 0
3.0813878 ABIN492382 EIA IF WB Mouse IgG2b AA 1-360, N-Term Log in to see AT1A4 0
3.0813878 ABIN126833 EIA WB Mouse IgG1 N-Term Log in to see 6H3 1
3.0813878 ABIN391367 FACS IHC (p) WB Rabbit Ig Fraction AA 154-183, Center Log in to see Polyclonal 3
3.0813878 ABIN126832 EIA IP WB Mouse IgG1 C-Term Log in to see 6G11 0
3.0813878 ABIN391720 IHC (p) WB Rabbit Ig Fraction AA 254-285, C-Term Log in to see Polyclonal 2
3.0813878 ABIN2869212 ELISA ICC IHC WB Mouse IgG2a Log in to see 4C11 1
3.0813878 ABIN438932 ICC IF IHC IHC (p) IP PLA WB Rabbit AA 310-360 Log in to see Polyclonal 0
3.0813878 ABIN1533683 ELISA WB Rabbit IgG AA 136-185 Log in to see Polyclonal 0
3.0813878 ABIN2855862 WB Rabbit IgG N-Term Log in to see Polyclonal 0
3.0813878 ABIN2983288 ELISA IF/ICC IHC WB Mouse IgG2a Log in to see 0
3.0813878 ABIN4309415 ICC IF IHC IHC (p) SimWes WB Rabbit IgG Log in to see Polyclonal 0
3.0813878 ABIN1873622 IF IHC WB Rabbit IgG N-Term Log in to see Polyclonal 0
3.0813878 ABIN3184547 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0

General

Antigen Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
Epitope AA 324-335, C-Term
(48), (22), (18), (15), (11), (10), (7), (6), (6), (5), (5), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(326), (135), (134), (39), (37), (19), (14), (10), (9), (9), (9), (7), (5), (4), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(176), (135), (16), (7), (3)
Clonality
Polyclonal
Conjugate This ERK2 antibody is un-conjugated
(11), (9), (7), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(304), (140), (115), (69), (60), (57), (27), (26), (10), (7), (7), (5), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-ERK2 Antibody

Target Details ERK2 Application Details Handling References for anti-ERK2 antibody (ABIN5518657) Images
Purpose Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase 1(MAPK1) detection. Tested with WB in Human,Mouse,Rat.
Sequence IAEAPFKFDM EL
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase 1(MAPK1) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: mitogen-activated protein kinase 1
Protein Name: Mitogen-activated protein kinase 1
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human ERK2(324-335aa IAEAPFKFDMEL), identical to the related rat and mouse sequences.
Isotype IgG
Plasmids, Primers & others

Target Details ERK2

Product Details anti-ERK2 Antibody Application Details Handling References for anti-ERK2 antibody (ABIN5518657) Images back to top
Antigen
Alternative Name MAPK1 (MAPK1 Antibody Abstract)
Background Mitogen-activated protein kinase 1, also known as MAPK1, p42MAPK, and ERK2, is an enzyme which in humans is encoded by the MAPK1 gene. The gene is mapped to 22q11.2. And The protein encoded by this gene is a member of the MAP kinase family. The deduced 360-amino acid human ERK2 protein shares 98 % identity with rat Erk2. The MAP kinase ERK2 is widely involved in eukaryotic signal transduction. Upon activation, it translocates to the nucleus of the stimulated cell, where it phosphorylates nuclear targets. Futhermore, the ERK pathway is necessary for experience-dependent plasticity and for long-term potentiation of synaptic transmission in the developing visual cortex. And the MAPK pathway is also involved in responses to NTN1.

Synonyms: ERK 2 antibody|ERK antibody|ERK-2 antibody|ERT 1 antibody|ERT1 antibody|Extracellular Signal Regulated Kinase 2 antibody|Extracellular signal-regulated kinase 2 antibody|MAP kinase 1 antibody|MAP kinase 2 antibody|MAP kinase 2 antibody|MAP kinase isoform p42 antibody|MAPK 1 antibody|MAPK 2 antibody|MAPK 2 antibody|MAPK1 antibody|MAPK2 antibody|Mitogen Activated Protein Kinase 1 antibody|Mitogen Activated Protein Kinase 2 antibody|Mitogen-activated protein kinase 1 antibody|Mitogen-activated protein kinase 2 antibody|MK01_HUMAN antibody|P38 antibody|P40 antibody|P41 antibody|P41MAPK antibody|p42 MAPK antibody|p42-MAPK antibody|P42MAPK antibody|P42MAPK antibody|PRKM 1 antibody|PRKM 2 antibody|PRKM1 antibody|PRKM2 antibody|Protein Tyrosine Kinase ERK2 antibody
UniProt P28482
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals

Application Details

Product Details anti-ERK2 Antibody Target Details ERK2 Handling References for anti-ERK2 antibody (ABIN5518657) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions For Research Use only

Handling

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details References for anti-ERK2 antibody (ABIN5518657) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Sodium azide, Thimerosal (Merthiolate)
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-ERK2 antibody (ABIN5518657)

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling Images back to top
Product cited in:

Li, Chen, Zhang, Chen, Nie, Xu, Gong, Shen, Su, Weng, Tan, Zhao, Zeng, Zhou: "SOX9 was involved in TKIs resistance in renal cell carcinoma via Raf/MEK/ERK signaling pathway." in: International journal of clinical and experimental pathology, Vol. 8, Issue 4, pp. 3871-81, 2015

Ma, Wang, Chen, Chen, Wang, Aisa: "Icariin and icaritin stimulate the proliferation of SKBr3 cells through the GPER1-mediated modulation of the EGFR-MAPK signaling pathway." in: International journal of molecular medicine, Vol. 33, Issue 6, pp. 1627-34, 2014

Images

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling References for anti-ERK2 antibody (ABIN5518657) back to top
Supplier Images
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 324-335), (C-Term) antibody (ABIN5518657) Anti-ERK2 antibody, All Western blottingAll lanes: Anti-MAPK1 at 0.5ug/ml Lane 1: Hu...