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|Antigen||Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies|
|Epitope||AA 252-270 Alternatives|
|Reactivity||Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This GJA1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
|5 references available|
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Product Details anti-GJA1 AntibodyTarget Details GJA1 Application Details Handling References for anti-GJA1 antibody (ABIN967717) Images
|Cross-Reactivity||Chicken, Dog (Canine), Human, Mouse (Murine)|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Rat Connexin-43 aa. 252-270|
Target Details GJA1Product Details anti-GJA1 Antibody Application Details Handling References for anti-GJA1 antibody (ABIN967717) Images back to top
|Alternative Name||Connexin-43 (GJA1 Antibody Abstract)|
|Background||Gap junctions are intercellular protein pores. They enable cell-to-cell communication by allowing passage of ions and other small molecules. The subunits of gap junction channels are assembled from a family of proteins called connexins. Individual connexin molecules join to make hexameric hemichannels termed connexons, these structures dock to connexons on neighboring cells, forming gap junction pores. Connexin-43 is a member of the connexin family possessing four transmembrane regions, with cytoplasmic amino and carboxyl terminals. It undergoes rapid turnover in the cell and its monomers may reside in the ER/Golgi network, forming a reservoir available for assembly upon degradation of existing connexin-43 channels. In addition, it is believed that phosphorylation of connexin-43 plays a regulatory role both in the assembly of connexons and in gating activity at the gap junction. This antibody is routinely tested by western blot analysis.|
|Molecular Weight||43 kDa|
|Research Area||Extracellular Matrix, Cytoskeleton, Signaling|
|Pathways||MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization|
Application DetailsProduct Details anti-GJA1 Antibody Target Details GJA1 Handling References for anti-GJA1 antibody (ABIN967717) Images back to top
Related Products: ABIN968545, ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-GJA1 Antibody Target Details GJA1 Application Details References for anti-GJA1 antibody (ABIN967717) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20° C.|
References for anti-GJA1 antibody (ABIN967717)Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling Images back to top
|Product cited in:||
Giepmans, Hengeveld, Postma, Moolenaar: "Interaction of c-Src with gap junction protein connexin-43. Role in the regulation of cell-cell communication." in: The Journal of biological chemistry, Vol. 276, Issue 11, pp. 8544-9, 2001
Toyofuku, Akamatsu, Zhang, Kuzuya, Tada, Hori: "c-Src regulates the interaction between connexin-43 and ZO-1 in cardiac myocytes." in: The Journal of biological chemistry, Vol. 276, Issue 3, pp. 1780-8, 2001
Lucke, Choudhry, Thom, Selmer, Burden, Hodgins: "Upregulation of connexin 26 is a feature of keratinocyte differentiation in hyperproliferative epidermis, vaginal epithelium, and buccal epithelium." in: The Journal of investigative dermatology, Vol. 112, Issue 3, pp. 354-61, 1999
Laird, Castillo, Kasprzak: "Gap junction turnover, intracellular trafficking, and phosphorylation of connexin43 in brefeldin A-treated rat mammary tumor cells." in: The Journal of cell biology, Vol. 131, Issue 5, pp. 1193-203, 1996
Loo, Berestecky, Kanemitsu, Lau: "pp60src-mediated phosphorylation of connexin 43, a gap junction protein." in: The Journal of biological chemistry, Vol. 270, Issue 21, pp. 12751-61, 1995
ImagesProduct Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling References for anti-GJA1 antibody (ABIN967717) back to top