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|Antigen||Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies|
|Reactivity||Bird (Avian), Dog (Canine), Chicken, Human, Monkey, Mouse (Murine), Rat (Rattus), Sheep (Ovine), Xenopus laevis, Zebrafish (Danio rerio) Alternatives|
|Conjugate||This GJA1 antibody is un-conjugated Alternatives|
Western Blotting (WB)
|1 reference available|
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Product Details anti-GJA1 AntibodyTarget Details GJA1 Application Details Handling References for anti-GJA1 antibody (ABIN318733) Images
|Specificity||This antibody recognises rat connexin43.|
|Immunogen||Synthetic peptide corresponding to the C-terminal region of rat connexin 43 (CT).|
Target Details GJA1Product Details anti-GJA1 Antibody Application Details Handling References for anti-GJA1 antibody (ABIN318733) Images back to top
|Alternative Name||GJA1 / Cx43 (GJA1 Antibody Abstract)|
|Background||Connexin43 is a 43 kDa member of the Alpha-type (group II) subfamily of the connexins. It is a component of gap junctions, the small channels linking the cytoplasm of adjacent cells and enabling signalling through the transfer of ions and metabolites. Phosphorylation of Connexin 43 at serine 368 regulates the permeability of the gap junction. Connexin 43 is the major protein of gap junctions in the heart, essential to synchronised contractions. It is also crucial in embryonic development and is associated with epilepsy.Synonyms: Connexin-43, GJAL, Gap junction 43 kDa heart protein, Gap junction alpha-1 protein|
|Pathways||MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization|
Application DetailsProduct Details anti-GJA1 Antibody Target Details GJA1 Handling References for anti-GJA1 antibody (ABIN318733) Images back to top
Western Blot: 1: 1000, detects a band of approximately 43 kDa in rat cerebellar lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-GJA1 Antibody Target Details GJA1 Application Details References for anti-GJA1 antibody (ABIN318733) Images back to top
|Buffer||10 mM Hepes pH 7.5, 150 mM NaCl containing 0.09 % Sodium Azide, 0.01 % Bovine Serum Albumin, 50 % Glycerol|
|Precaution of Use||This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-GJA1 antibody (ABIN318733)Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling Images back to top
Bao, Reuss, Altenberg: "Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368." in: The Journal of biological chemistry, Vol. 279, Issue 19, pp. 20058-66, 2004