anti-Chimpanzee HSPB1 antibody for Immunofluorescence

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • 27kDa
  • Hsp27
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • heat shock protein family B (small) member 1
  • heat shock protein 1
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock protein family B (small) member 1 L homeolog
  • HSPB1
  • Hspb1
  • hspb1
  • hspb1.L
Reactivity
Chicken, Chimpanzee, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
986
368
342
157
128
104
89
68
67
55
49
44
36
30
13
11
6
4
3
2
2
2
1
1
1
1
Host
Mouse
562
444
3
Clonality
Monoclonal
Conjugate
This HSPB1 antibody is conjugated to CF®555
42
40
29
28
24
18
16
13
13
12
10
10
10
10
10
9
9
9
8
8
8
8
8
8
8
8
8
6
6
6
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt), Western Blotting (WB)
865
450
304
288
277
236
216
212
75
58
41
41
40
18
14
6
5
4
2
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Catalog No. ABIN6164179
$ 209.23
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN6164147 FACS IF IHC (f) ISt WB CF®770 Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164155 FACS IF IHC (f) ISt WB CF®594 Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164173 FACS IF IHC (f) ISt WB CF®640R Mouse IgG1, kappa Log in to see HSPB1-774 0
1 ABIN6164171 FACS IF IHC (f) ISt WB CF405M Mouse IgG1, kappa Log in to see HSPB1-774 0
1 ABIN6164175 FACS IF IHC (f) ISt WB CF®543 Mouse IgG1, kappa Log in to see HSPB1-774 0
1 ABIN6164183 FACS IF IHC (f) ISt WB CF®568 Mouse IgG1, kappa Log in to see HSPB1-774 0
1 ABIN6164185 FACS IF IHC (f) ISt WB CF®770 Mouse IgG1, kappa Log in to see HSPB1-774 0
1 ABIN6164187 FACS IF IHC (f) ISt WB CF®680 Mouse IgG1, kappa Log in to see HSPB1-774 0
1 ABIN6164189 FACS IF IHC (f) ISt WB CF®680R Mouse IgG1, kappa Log in to see HSPB1-774 0
1 ABIN6164193 FACS IF IHC (f) ISt WB CF®594 Mouse IgG1, kappa Log in to see HSPB1-774 0
1 ABIN6164153 FACS IF IHC (f) ISt WB CF®488A Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164133 FACS IF IHC (f) ISt WB CF405M Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164135 FACS IF IHC (f) ISt WB CF®640R Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164141 FACS IF IHC (f) ISt WB CF®555 Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164131 FACS IF IHC (f) ISt WB CF®405S Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164149 FACS IF IHC (f) ISt WB CF®680 Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164151 FACS IF IHC (f) ISt WB CF®680R Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164143 FACS IF IHC (f) ISt WB CF®660R Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164145 FACS IF IHC (f) ISt WB CF®568 Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164177 FACS IF IHC (f) ISt WB CF®647 Mouse IgG1, kappa Log in to see HSPB1-774 0

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Reactivity Chicken, Chimpanzee, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
(986), (368), (342), (157), (128), (104), (89), (68), (67), (55), (49), (44), (36), (30), (13), (11), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(562), (444), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HSPB1 antibody is conjugated to CF®555
(42), (40), (29), (28), (24), (18), (16), (13), (13), (12), (10), (10), (10), (10), (10), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (6), (6), (6), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt), Western Blotting (WB)
(865), (450), (304), (288), (277), (236), (216), (212), (75), (58), (41), (41), (40), (18), (14), (6), (5), (4), (2), (2), (1), (1), (1)
Supplier Log in to see

Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling Images
Purpose Mouse Monoclonal anti-HSP27 (HSPB1/774), CF555 Conjugate
Characteristics This antibody recognizes a 24-27 kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced ?p29? and ?24K? protein. About 50 % of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen Recombinant human HSPB1 protein
Clone HSPB1-774
Isotype IgG1, kappa
Plasmids, Primers & others

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Molecular Weight 27 kDa
Gene ID 3315, 520973
UniProt P04792
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Application Notes Immunohistology formalin-fixed 0.5-1 μg/mL
  • Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Immunofluorescence 0.5-1 μg/mL
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Western blotting 0.25-0.5 μg/mL
  • Optimal dilution for a specific application should be determined by user
Comment

~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR.

Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light