anti-Chimpanzee HSPB1 antibody for Immunofluorescence

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • 27kDa
  • Hsp27
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • heat shock protein family B (small) member 1
  • heat shock protein 1
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock protein family B (small) member 1 L homeolog
  • HSPB1
  • Hspb1
  • hspb1
  • hspb1.L
Reactivity
Chicken, Chimpanzee, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
825
276
271
95
84
71
68
66
36
30
29
26
18
11
11
8
6
3
2
2
2
2
1
1
1
Host
Mouse
503
340
2
Clonality
Monoclonal
Conjugate
This HSPB1 antibody is un-conjugated
34
33
23
22
19
13
13
13
11
11
10
10
10
10
10
9
9
9
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
714
326
270
266
227
212
169
156
59
40
17
14
11
8
6
4
2
2
1
1
1
Supplier
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Catalog No. ABIN3026467
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3025020 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see HSPB1-774
1 ABIN3025018 FACS IF IHC WB Mouse IgG1, kappa Log in to see SPM252
1 ABIN2741544 ELISA FACS IF IHC (p) IP WB Mouse IgG1, kappa Log in to see G3-1
1 ABIN3025019 FACS IF IHC WB Mouse IgG1, kappa Log in to see SPM252
1 ABIN3025022 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see HSPB1-774
1 ABIN3026469 FACS IF IHC WB Mouse IgG1, kappa Log in to see G3-1

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Reactivity Chicken, Chimpanzee, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
(825), (276), (271), (95), (84), (71), (68), (66), (36), (30), (29), (26), (18), (11), (11), (8), (6), (3), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(503), (340), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HSPB1 antibody is un-conjugated
(34), (33), (23), (22), (19), (13), (13), (13), (11), (11), (10), (10), (10), (10), (10), (9), (9), (9), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(714), (326), (270), (266), (227), (212), (169), (156), (59), (40), (17), (14), (11), (8), (6), (4), (2), (2), (1), (1), (1)
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling Images
Characteristics This antibody recognizes a 24-27 kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). HSP27 was recently found to be identical to the estrogen-induced "p29" and "24K" protein. About 50 % of breast carcinomas are positive for HSP27, especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Purification Protein G affinity chromatography
Immunogen Partially purified human HSP27 (earlier called 24K) from breast cancer MCF-7 cells was used as the immunogen for this antibody.
Clone G3-1
Isotype IgG1, kappa

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background This antibody recognizes a 24-27 kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). HSP27 was recently found to be identical to the estrogen-induced ""p29"" and ""24K"" protein. About 50 % of breast carcinomas are positive for HSP27, especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Research Area Heat Shock Proteins
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Application Notes The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,WB: 0.25-0.5 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the HSP27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (ABIN3026467) IHC testing of breast carcinoma (20X) stained with HSP27 antibody (G3.1).
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (ABIN3026467) IHC testing of FFPE human prostate carcinoma and HSP27 antibody.
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (ABIN3026467) IHC testing of human breast carcinoma (10X) stained with HSP27 antibody (G3.1).