anti-Monkey HSPB1 antibody for Immunofluorescence

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • 27kDa
  • Hsp27
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • heat shock protein family B (small) member 1
  • heat shock protein 1
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock protein family B (small) member 1 L homeolog
  • HSPB1
  • Hspb1
  • hspb1
  • hspb1.L
Reactivity
Chicken, Chimpanzee, Human, Monkey, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
992
373
347
157
128
104
89
68
66
55
49
44
36
30
13
11
7
4
3
2
2
2
1
1
1
1
Host
Mouse
568
444
3
Clonality
Monoclonal
Conjugate
This HSPB1 antibody is conjugated to CF®405S
42
40
29
28
24
18
16
13
13
12
10
10
10
10
10
9
9
9
8
8
8
8
8
8
8
8
8
6
6
6
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt), Western Blotting (WB)
871
451
309
289
284
236
218
216
75
59
41
41
40
18
14
6
5
4
2
2
1
1
1
Supplier
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Catalog No. ABIN6164131
$ 209.23
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN6164141 FACS IF IHC (f) ISt WB CF®555 Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164147 FACS IF IHC (f) ISt WB CF®770 Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164151 FACS IF IHC (f) ISt WB CF®680R Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164135 FACS IF IHC (f) ISt WB CF®640R Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164139 FACS IF IHC (f) ISt WB CF®647 Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164145 FACS IF IHC (f) ISt WB CF®568 Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164149 FACS IF IHC (f) ISt WB CF®680 Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164133 FACS IF IHC (f) ISt WB CF405M Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164137 FACS IF IHC (f) ISt WB CF®543 Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164143 FACS IF IHC (f) ISt WB CF®660R Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164153 FACS IF IHC (f) ISt WB CF®488A Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164155 FACS IF IHC (f) ISt WB CF®594 Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164166 FACS IF IHC (f) ISt WB Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164157 FACS IF IHC (f) ISt WB APC Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164160 FACS IF IHC (f) ISt WB Biotin Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164164 FACS IF IHC (f) ISt WB PerCP Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164158 FACS IF IHC (f) ISt WB Alkaline Phosphatase (AP) Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164165 FACS IF IHC (f) ISt WB PE Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164168 FACS IF IHC (f) ISt WB Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN222927 IF IP WB Rabbit IgG Log in to see Polyclonal 1

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Reactivity Chicken, Chimpanzee, Human, Monkey, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
(992), (373), (347), (157), (128), (104), (89), (68), (66), (55), (49), (44), (36), (30), (13), (11), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(568), (444), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HSPB1 antibody is conjugated to CF®405S
(42), (40), (29), (28), (24), (18), (16), (13), (13), (12), (10), (10), (10), (10), (10), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (6), (6), (6), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt), Western Blotting (WB)
(871), (451), (309), (289), (284), (236), (218), (216), (75), (59), (41), (41), (40), (18), (14), (6), (5), (4), (2), (2), (1), (1), (1)
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling Images
Purpose Mouse Monoclonal anti-HSP27 (G3.1), CF405S Conjugate
Characteristics This MAb reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50 % homology with low molecular weight Drosophila HSPs and mammalian α-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore HSP27 may be useful in classifying various hormone sensitive tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
Clone G3-1
Isotype IgG1, kappa
Plasmids, Primers & others

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Molecular Weight 27 kDa
Gene ID 3315, 520973
UniProt P04792
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Application Notes Immunohistology formalin-fixed 0.5-1 μg/mL
  • Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Immunofluorescence 0.5-1 μg/mL
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Western blotting 0.25-0.5 μg/mL
  • Optimal dilution for a specific application should be determined by user
Comment

~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR.

Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light