anti-Mouse (Murine) HSPB1 antibody for Immunofluorescence

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • 27kDa
  • Hsp27
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • heat shock protein family B (small) member 1
  • heat shock protein 1
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock protein family B (small) member 1 L homeolog
  • HSPB1
  • Hspb1
  • hspb1
  • hspb1.L
Epitope
Center
85
69
60
45
22
18
18
16
16
13
13
8
7
6
6
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
992
373
347
158
129
104
89
68
67
56
49
44
36
30
13
11
7
4
3
2
2
2
1
1
1
1
Host
Rabbit
567
445
3
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
42
40
29
28
24
18
16
13
13
12
10
10
10
10
10
9
9
9
8
8
8
8
8
8
8
8
8
6
6
6
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
871
451
309
288
284
236
217
215
75
59
42
42
40
18
14
6
5
4
2
2
1
1
1
Options
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Catalog No. ABIN2854951
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.32649 ABIN4319980 ICC IF IHC IHC (p) WB Rabbit AA 80-150, Internal Region Log in to see Polyclonal 2
11.32649 ABIN4320008 CyTOF FACS ICC IF WB Mouse IgG1 Log in to see 6H11 0
11.32649 ABIN4320010 ICC IF WB Chicken Log in to see Polyclonal 0
11.32649 ABIN1873103 IF WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN361756 FACS ICC IF IHC IP WB Mouse IgG1 kappa Log in to see 8A7 9
1 ABIN1027725 FACS ICC IF WB Rabbit Log in to see Polyclonal 7
1 ABIN2484350 FACS ICC IF WB Atto 655 Rabbit Log in to see Polyclonal 6
1 ABIN2484356 FACS ICC IF WB FITC Rabbit Log in to see Polyclonal 6
1 ABIN2484358 FACS ICC IF WB PE-Atto 594 Rabbit Log in to see Polyclonal 6
1 ABIN2484355 FACS ICC IF WB Biotin Rabbit Log in to see Polyclonal 6
1 ABIN2484360 FACS ICC IF WB PE Rabbit Log in to see Polyclonal 6
1 ABIN2484359 FACS ICC IF WB PerCP Rabbit Log in to see Polyclonal 6
1 ABIN2484347 FACS ICC IF WB Atto 565 Rabbit Log in to see Polyclonal 6
1 ABIN2484349 FACS ICC IF WB Atto 633 Rabbit Log in to see Polyclonal 6
1 ABIN2484346 FACS ICC IF WB Atto 488 Rabbit Log in to see Polyclonal 6
1 ABIN2484352 FACS ICC IF WB Atto 700 Rabbit Log in to see Polyclonal 6
1 ABIN2484345 FACS ICC IF WB Atto 390 Rabbit Log in to see Polyclonal 6
1 ABIN2484348 FACS ICC IF WB Atto 594 Rabbit Log in to see Polyclonal 6
1 ABIN2484353 FACS ICC IF WB Alkaline Phosphatase (AP) Rabbit Log in to see Polyclonal 6
1 ABIN2484351 FACS ICC IF WB Atto 680 Rabbit Log in to see Polyclonal 6

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope Center
(85), (69), (60), (45), (22), (18), (18), (16), (16), (13), (13), (8), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(992), (373), (347), (158), (129), (104), (89), (68), (67), (56), (49), (44), (36), (30), (13), (11), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(567), (445), (3)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is un-conjugated
(42), (40), (29), (28), (24), (18), (16), (13), (13), (12), (10), (10), (10), (10), (10), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (6), (6), (6), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(871), (451), (309), (288), (284), (236), (217), (215), (75), (59), (42), (42), (40), (18), (14), (6), (5), (4), (2), (2), (1), (1), (1)
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling Images
Cross-Reactivity Rhesus Monkey
Cross-Reactivity (Details) Rhesus Monkey (100 %)
Characteristics Rabbit Polyclonal antibody to HSP27 (heat shock 27 kDa protein 1)
HSP27 antibody
Purification Affinity purified by Protein A.
Immunogen Recombinant protein encompassing a sequence within the center region of human HSP27. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).

Cellular Localization: Cytoplasm , Nucleus , Spindle
Molecular Weight 23 kDa
Gene ID 3315
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Application Notes Suggested dilution Reference Flow cytometry/FACS Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceFlow cytometry/FACSAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:1000* Western blot1:500-1:10000*
Comment

Positive Control: A431 , HeLa , mouse liver , *HT29

Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
Format Liquid
Concentration 3.23 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
Preservative ProClin
Precaution of Use This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibody (ABIN2854951) WB Image Sample (50 ug of whole cell lysate) A: mouse liver 12% SDS PAGE antibody dil...
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibody (ABIN2854951) IHC-P Image HSP27 antibody detects HSPB1 protein at cytosol on U87 xenograft by immun...
Immunoprecipitation (IP) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibody (ABIN2854951) IP Image Immunoprecipitation of HSP27 protein from HeLa whole cell extracts using 5 μ...
Western Blotting (WB) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibody (ABIN2854951) WB Image HSP27 antibody detects HSP27 protein by western blot analysis. A. 30 μg A431...
Immunofluorescence (IF) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibody (ABIN2854951) ICC/IF Image HSP27 antibody detects HSP27 protein at cytoplasm and cytoskeleton by im...