anti-Mouse (Murine) HSPB1 antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • 27kDa
  • Hsp27
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • heat shock protein family B (small) member 1
  • heat shock protein 1
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock protein family B (small) member 1 L homeolog
  • HSPB1
  • Hspb1
  • hspb1
  • hspb1.L
Epitope
AA 100-150
83
68
58
26
22
17
17
15
15
13
12
9
7
6
6
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
968
373
345
162
133
102
86
67
67
61
47
42
36
29
13
11
7
4
3
2
2
2
1
1
1
1
Host
Rabbit
557
429
3
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
41
39
27
27
22
18
14
12
12
11
9
9
9
9
9
8
8
8
8
8
8
8
8
8
8
8
8
6
6
6
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
841
429
290
286
261
239
215
211
75
59
42
42
39
18
14
6
5
4
2
2
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Pubmed
2 references available

Available images

Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 100-150) antibody (ABIN672441)
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 100-150) antibody (ABIN672441)
SDS-PAGE (SDS) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 100-150) antibody (ABIN672441)
Immunofluorescence (IF) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 100-150) antibody (ABIN672441)
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 100-150) antibody (ABIN672441)
image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 100-150) antibody (ABIN672441)
Catalog No. ABIN672441
$ 318.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN901432 IF (p) Alexa Fluor 647 Rabbit IgG AA 100-150 Log in to see Polyclonal 0
1 ABIN672447 IF (p) Cy7 Rabbit IgG AA 100-150 Log in to see Polyclonal 0
1 ABIN901431 IF (p) Alexa Fluor 555 Rabbit IgG AA 100-150 Log in to see Polyclonal 0
1 ABIN672445 IF (p) Cy5 Rabbit IgG AA 100-150 Log in to see Polyclonal 0
1 ABIN2814545 IF (p) Alexa Fluor 594 Rabbit IgG AA 100-150 Log in to see Polyclonal 0
1 ABIN672444 IF (p) Cy3 Rabbit IgG AA 100-150 Log in to see Polyclonal 0
1 ABIN672446 IF (p) Cy5.5 Rabbit IgG AA 100-150 Log in to see Polyclonal 0
1 ABIN672448 IF (p) FITC Rabbit IgG AA 100-150 Log in to see Polyclonal 0
1 ABIN901429 IF (p) Alexa Fluor 488 Rabbit IgG AA 100-150 Log in to see Polyclonal 0
1 ABIN901433 IF (p) Alexa Fluor 350 Rabbit IgG AA 100-150 Log in to see Polyclonal 0
1 ABIN877140 IF (p) Alexa Fluor 350 Rabbit IgG pSer82 Log in to see Polyclonal 0
1 ABIN877142 IF (p) Alexa Fluor 555 Rabbit IgG pSer82 Log in to see Polyclonal 0
1 ABIN877141 IF (p) Alexa Fluor 647 Rabbit IgG pSer82 Log in to see Polyclonal 0
1 ABIN685324 IF (p) Cy7 Rabbit IgG pSer82 Log in to see Polyclonal 0
1 ABIN877138 IF (p) Alexa Fluor 488 Rabbit IgG pSer82 Log in to see Polyclonal 0
1 ABIN685322 IF (p) Cy5 Rabbit IgG pSer82 Log in to see Polyclonal 0
1 ABIN685323 IF (p) Cy5.5 Rabbit IgG pSer82 Log in to see Polyclonal 0
1 ABIN685321 IF (p) Cy3 Rabbit IgG pSer82 Log in to see Polyclonal 0
1 ABIN685325 IF (p) FITC Rabbit IgG pSer82 Log in to see Polyclonal 0
1 ABIN2814547 IF (p) Alexa Fluor 594 Rabbit IgG pSer82 Log in to see Polyclonal 0

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope AA 100-150
(83), (68), (58), (26), (22), (17), (17), (15), (15), (13), (12), (9), (7), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(968), (373), (345), (162), (133), (102), (86), (67), (67), (61), (47), (42), (36), (29), (13), (11), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(557), (429), (3)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is un-conjugated
(41), (39), (27), (27), (22), (18), (14), (12), (12), (11), (9), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (6), (6), (6), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(841), (429), (290), (286), (261), (239), (215), (211), (75), (59), (42), (42), (39), (18), (14), (6), (5), (4), (2), (2), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling References for anti-HSPB1 antibody (ABIN672441) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human HSP27
Isotype IgG
Plasmids, Primers & others

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 antibody (ABIN672441) Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background

Heat Shock Protein 27 (HSP27) is a 27 kDa member of a family of proteins whose expression and function are stimulated by heat shock and other stress stimuli. A major function of these proteins is to serve as chaperones that bind to and stabilize the active conformation of other proteins. HSP27, along with other members of the small HSP group, possesses a C-terminal Alpha-crystalline homology domain. HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Cytoplasmic HSP27 exists in multiple complexes. One complex consists of HSP27, Akt (PKB), MAPKAP-kinase 2, and p38 MAPK. The presence of HSP27 in this complex is required for Akt activation by stress stimuli. Another complex consists of HSP27 and the IKK complex. HSP27 is also an actin capping protein that binds to the barbed (growing) ends of actin filaments, thereby inhibiting filament extension. Phosphorylation of HSP27 on serine 82 by MAPKAP-kinase 2 leads to HSP27 dissociation from the Akt/MAPKAP-kinase 2/p38 complex and from actin filaments, and stimulates HSP27 binding to the IKK complex.

Subcellular location: Cytoplasm, Nucleus

Synonyms: CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.7667, HEL-S-12, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27
Gene ID 3315
UniProt P04792
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 antibody (ABIN672441) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 antibody (ABIN672441) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-HSPB1 antibody (ABIN672441)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Product cited in:

Gao, Liu, Chen, Lv, Wu, Mi, Wang: "Comparative study of Hsp27, GSK3?, Wnt1 and PRDX3 in Hirschsprung's disease." in: International journal of experimental pathology, Vol. 95, Issue 3, pp. 229-37, 2014 Method employed by authors: Western Blotting (WB) (Sample species: Human). Further details: Sample: Colon

Wang, Yang, Liu, Zhou, Wu, Qiao, Li, Wang: "Dietary supplementation with the probiotic Lactobacillus fermentum I5007 and the antibiotic aureomycin differentially affects the small intestinal proteomes of weanling piglets." in: The Journal of nutrition, Vol. 142, Issue 1, pp. 7-13, 2011

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 antibody (ABIN672441) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 100-150) antibody (ABIN672441) Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-HSP27/HspB1/H...
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 100-150) antibody (ABIN672441) Formalin-fixed and paraffin embedded rat brain labeled with (ABIN672441) Rabbit Ant...
SDS-PAGE (SDS) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 100-150) antibody (ABIN672441) L1 mouse brain lysates L2 mouse liver lysates probed with Anti HSP27/HspB1/HSP25 Poly...
Immunofluorescence (IF) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 100-150) antibody (ABIN672441) Formalin-fixed and paraffin-embedded rat brain labeled with Anti-HSP27/HspB1/HSP25 Po...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 100-150) antibody (ABIN672441) Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-HSP27/HspB1/H...
image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 100-150) antibody (ABIN672441) A431 cells probed with HSP27 Polyclonal Antibody, unconjugated at 1:100 dilution for...