anti-Shark HSPB1 antibody for Flow Cytometry

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • 27kDa
  • Hsp27
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • heat shock protein family B (small) member 1
  • heat shock protein 1
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock protein family B (small) member 1 L homeolog
  • HSPB1
  • Hspb1
  • hspb1
  • hspb1.L
Reactivity
Dog (Canine), Human, Mouse (Murine), Shark
967
373
346
162
133
101
86
67
66
61
47
42
35
29
13
11
7
4
3
2
2
2
1
1
1
1
Host
Rabbit
557
428
3
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is conjugated to Atto 488
41
39
27
27
22
18
14
12
12
11
9
9
9
9
9
8
8
8
8
8
8
8
8
8
8
8
7
6
6
6
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
840
429
290
285
261
238
214
211
75
58
42
42
40
18
14
6
5
4
2
2
1
1
1
Options
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Catalog No. ABIN2484346
$ 504.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
21.778215 ABIN2484357 FACS ICC IF WB HRP Rabbit Log in to see Polyclonal 6
21.778215 ABIN2484351 FACS ICC IF WB Atto 680 Rabbit Log in to see Polyclonal 6
21.778215 ABIN2484345 FACS ICC IF WB Atto 390 Rabbit Log in to see Polyclonal 6
21.778215 ABIN2484347 FACS ICC IF WB Atto 565 Rabbit Log in to see Polyclonal 6
21.778215 ABIN2484350 FACS ICC IF WB Atto 655 Rabbit Log in to see Polyclonal 6
21.778215 ABIN2484348 FACS ICC IF WB Atto 594 Rabbit Log in to see Polyclonal 6
21.778215 ABIN2484349 FACS ICC IF WB Atto 633 Rabbit Log in to see Polyclonal 6
21.778215 ABIN2484352 FACS ICC IF WB Atto 700 Rabbit Log in to see Polyclonal 6
21.778215 ABIN2484353 FACS ICC IF WB Alkaline Phosphatase (AP) Rabbit Log in to see Polyclonal 6
21.778215 ABIN2484354 FACS ICC IF WB APC Rabbit Log in to see Polyclonal 6
21.778215 ABIN2484355 FACS ICC IF WB Biotin Rabbit Log in to see Polyclonal 6
21.778215 ABIN2484361 FACS ICC IF WB Streptavidin Rabbit Log in to see Polyclonal 6
21.778215 ABIN2484358 FACS ICC IF WB PE-Atto 594 Rabbit Log in to see Polyclonal 6
21.778215 ABIN2484359 FACS ICC IF WB PerCP Rabbit Log in to see Polyclonal 6
21.778215 ABIN2484360 FACS ICC IF WB PE Rabbit Log in to see Polyclonal 6
21.778215 ABIN2484356 FACS ICC IF WB FITC Rabbit Log in to see Polyclonal 6
20.278215 ABIN1027725 FACS ICC IF WB Rabbit Log in to see Polyclonal 7
1 ABIN5867982 FACS IF/ICC WB APC Rabbit Log in to see Polyclonal 0
1 ABIN5867991 FACS IF/ICC WB Alkaline Phosphatase (AP) Rabbit Log in to see Polyclonal 0
1 ABIN5867986 FACS IF/ICC WB Atto 594 Rabbit Log in to see Polyclonal 0

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Reactivity Dog (Canine), Human, Mouse (Murine), Shark
(967), (373), (346), (162), (133), (101), (86), (67), (66), (61), (47), (42), (35), (29), (13), (11), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(557), (428), (3)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is conjugated to Atto 488
(41), (39), (27), (27), (22), (18), (14), (12), (12), (11), (9), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (7), (6), (6), (6), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(840), (429), (290), (285), (261), (238), (214), (211), (75), (58), (42), (42), (40), (18), (14), (6), (5), (4), (2), (2), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling References for anti-HSPB1 antibody (ABIN2484346) Images
Specificity Detects ~27 kDa.
Purification Protein A Purified
Immunogen Human HSP27, His tagged
Plasmids, Primers & others

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 antibody (ABIN2484346) Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background HSP27s belong to an abundant and ubiquitous family of small heat shock proteins (sHSP). It is an important HSP found in both normal human cells and cancer cells. The basic structure of most sHSPs is a homologous and highly conserved amino acid sequence, with an α-crystallin domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (1, 2). HSP27-oligomers consist of stable dimers formed by as many as 8-40 HSP27 protein monomers (3). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (4). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Other functions include chaperone activity (as mentioned above), thermo tolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. HSP27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that HSP27 may serve some role in cross-bridge formation between actin and myosin (5). And finally, HSP27 is also thought to be involved in the process of cell differentiation. The up-regulation of HSP27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that HSP27 may play a crucial role in termination of growth (6). Looking for more information on HSP27? Visit our new HSP27 Scientific Resource Guide at http://www.HSP27.com.
Gene ID 3315
NCBI Accession NP_001531
UniProt P04792
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 antibody (ABIN2484346) Images back to top
Application Notes
  • WB (1:2000)
  • ICC/IF (1:250)
  • optimal dilutions for assays should be determined by the user.
Comment

A 1:2000 dilution of SPC-106 was sufficient for detection of HSP27 in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 antibody (ABIN2484346) Images back to top
Format Liquid
Concentration 1.38 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-HSPB1 antibody (ABIN2484346)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Background publications

Arrigo: "In search of the molecular mechanism by which small stress proteins counteract apoptosis during cellular differentiation." in: Journal of cellular biochemistry, Vol. 94, Issue 2, pp. 241-6, 2005

Van Montfort, Slingsby, Vierling: "Structure and function of the small heat shock protein/alpha-crystallin family of molecular chaperones." in: Advances in protein chemistry, Vol. 59, pp. 105-56, 2002

Sarto, Binz, Mocarelli: "Heat shock proteins in human cancer." in: Electrophoresis, Vol. 21, Issue 6, pp. 1218-26, 2000

Kim, Kim, Kim: "Crystal structure of a small heat-shock protein." in: Nature, Vol. 394, Issue 6693, pp. 595-9, 1998

Ehrnsperger, Gräber, Gaestel, Buchner: "Binding of non-native protein to Hsp25 during heat shock creates a reservoir of folding intermediates for reactivation." in: The EMBO journal, Vol. 16, Issue 2, pp. 221-9, 1997

Ciocca, Oesterreich, Chamness, McGuire, Fuqua: "Biological and clinical implications of heat shock protein 27,000 (Hsp27): a review." in: Journal of the National Cancer Institute, Vol. 85, Issue 19, pp. 1558-70, 1993

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 antibody (ABIN2484346) back to top
Supplier Images
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (Atto 488) (ABIN2484346) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp27 Polyclonal An...
Western Blotting (WB) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (Atto 488) (ABIN2484346) Western blot analysis of Human HeLa cell lysates showing detection of HSP27 protein u...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (Atto 488) (ABIN2484346) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp27 Polyclonal An...