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|Antigen||Mitogen-Activated Protein Kinase 8 (MAPK8) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This JNK antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-JNK AntibodyTarget Details JNK Application Details Handling Images
|Specificity||This antibody specifically interacts with the Thr - Gly/Pro - pTyr motif of activated, monophosphorylated SAPK1/2 kinases. The antibody accepts the dually phosphorylated site as well (pThr - Gly/Pro - pTyr) and does not interact with the non-phosphorylated form of the protein. Mab SAPK1/2-9H8 shows no crossreaction with activated MAP Kinases 1 or 2.|
|Characteristics||Synonyms: Mitogen-activated protein kinase 8, c-Jun N-terminal kinase 1, JNK-46, JNK-1, PRKM8,Stress-activated protein kinase JNK1|
|Purification||Size exclusion chromatography|
|Components||incl. positive Control|
|Immunogen||Synthetic peptide conjugated to KLH.|
Target Details JNKProduct Details anti-JNK Antibody Application Details Handling Images back to top
|Alternative Name||MAPK8 / JNK1 (MAPK8 Antibody Abstract)|
|Background||Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines.Synonyms: JNK-1, JNK-46, Mitogen-activated protein kinase 8, PRKM8, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1|
|Pathways||MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-JNK Antibody Target Details JNK Handling Images back to top
Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from sorbit-treated A431 cells. ELISA: 0.05 μg/mL. Immunoprecipitation: 1 - 10 g per 10^6 pervanadate-treated A431 cells. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-JNK Antibody Target Details JNK Application Details Images back to top
|Reconstitution||Restore with 1 mL H2O (15 min, RT).|
|Buffer||1 mL2 x PBS / 0.1 % Na-azide / PEG and Sucrose|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C/-80 °C|
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-JNK Antibody Target Details JNK Application Details Handling back to top