anti-Pig (Porcine) JNK antibody for Immunocytochemistry

Recommended JNK Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 8 (MAPK8) Antibodies
  • JNK
  • JNK-46
  • JNK1
  • JNK1A2
  • JNK21B1/2
  • PRKM8
  • SAPK1
  • SAPK1c
  • AI849689
  • Prkm8
  • BSK
  • BSK/DJNK
  • Bsk
  • CG5680
  • D-JNK
  • D-junk
  • DBSK/JNK
  • DJNK
  • DJNK/bsk
  • Dmel\\CG5680
  • JNK/SAPK
  • Jnk
  • Junk
  • SAPKa
  • c-Jun
  • dJNK
  • jnk
  • T10F20.15
  • jnk1
  • sapk1
  • mapk8
  • zgc:112379
  • JNKb
  • mapk8b
  • mitogen-activated protein kinase 8
  • Stress-activated protein kinase jnk-1
  • basket
  • Protein kinase superfamily protein
  • mitogen-activated protein kinase 8 L homeolog
  • mitogen-activated protein kinase 8b
  • mitogen-activated protein kinase 8B-like
  • MAPK8
  • jnk-1
  • Mapk8
  • bsk
  • ATMPK8
  • mapk8.L
  • mapk8b
  • LOC109094263
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
430
198
167
25
20
15
10
8
6
5
4
3
3
2
2
2
2
1
1
1
Host
Rabbit
347
101
3
2
Clonality
Polyclonal
Conjugate
This JNK antibody is un-conjugated
21
19
15
12
11
11
4
3
3
3
3
3
3
3
3
3
3
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
350
206
126
82
73
66
39
39
25
19
15
15
4
3
3
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN6262710
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2625183 ICC IHC IHC (p) WB Rabbit IgG AA 344-363 Log in to see Polyclonal 0
1 ABIN2610988 ICC IF IHC IHC (p) WB Rabbit pThr183 Log in to see Polyclonal 0

General

Antigen Mitogen-Activated Protein Kinase 8 (MAPK8) Antibodies
Reactivity Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(430), (198), (167), (25), (20), (15), (10), (8), (6), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(347), (101), (3), (2)
Clonality
Polyclonal
Conjugate This JNK antibody is un-conjugated
(21), (19), (15), (12), (11), (11), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(350), (206), (126), (82), (73), (66), (39), (39), (25), (19), (15), (15), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-JNK Antibody

Target Details JNK Application Details Handling References for anti-JNK antibody (ABIN6262710) Images
Specificity JNK1/2/3 Antibody detects endogenous levels of total JNK1/2/3
Cross-Reactivity Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
Purification The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Immunogen A synthesized peptide derived from human JNK1/2/3
Isotype IgG
Plasmids, Primers & others

Target Details JNK

Product Details anti-JNK Antibody Application Details Handling References for anti-JNK antibody (ABIN6262710) Images back to top
Antigen
Alternative Name JNK1/2/3 (MAPK8 Antibody Abstract)
Background

Description: Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PubMed:10747973).

Gene: MAPK8

Molecular Weight 46+54kDa
Gene ID 5599
UniProt P45983, P45984, P53779
Pathways MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-JNK Antibody Target Details JNK Handling References for anti-JNK antibody (ABIN6262710) Images back to top
Application Notes WB 1:500-1:2000 IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-JNK Antibody Target Details JNK Application Details References for anti-JNK antibody (ABIN6262710) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

References for anti-JNK antibody (ABIN6262710)

Product Details anti-JNK Antibody Target Details JNK Application Details Handling Images back to top
Product cited in:

Geng, Yang, Wang, Zhang, Hu, Gu, Li: "Trimethylamine N-oxide promotes atherosclerosis via CD36-dependent MAPK/JNK pathway." in: Biomedicine & pharmacotherapy, Vol. 97, pp. 941-947, 2018

Ji, Yuan, Yuan, Xia, Yang: "The p75 neurotrophin receptor might mediate sepsis-induced synaptic and cognitive impairments." in: Behavioural brain research, Vol. 347, pp. 339-349, 2018

Zou, Xiang, Wang, Peng, Wei: "Oregano Essential Oil Improves Intestinal Morphology and Expression of Tight Junction Proteins Associated with Modulation of Selected Intestinal Bacteria and Immune Status in a Pig Model." in: BioMed research international, Vol. 2016, pp. 5436738, 2017

Images

Product Details anti-JNK Antibody Target Details JNK Application Details Handling References for anti-JNK antibody (ABIN6262710) back to top
Supplier Images
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Mitogen-Activated Protein Kinase 8 (MAPK8) antibody (ABIN6262710) ABIN6269260 staining lovo cells by ICC/IF. Cells were fixed with PFA and permeabilize...
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 8 (MAPK8) antibody (ABIN6262710) Western blot analysis of JNK1/2/3 expression in UV treated 293 whole cell lysates,The...