anti-Human MAP2K1 antibody for Immunofluorescence (Cultured Cells)

Recommended MAP2K1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
  • Mek1
  • CFC3
  • MAPKK1
  • MEK1
  • MKK1
  • PRKMK1
  • MEKK1
  • Prkmk1
  • mek-2
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
  • ATMEK1
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • NMAPKK
  • MEK
  • mitogen activated protein kinase kinase 1
  • mitogen-activated protein kinase kinase 1
  • mitogen-activated protein kinase kinase 1 L homeolog
  • MAP kinase/ ERK kinase 1
  • Map2k1
  • MAP2K1
  • map2k1.L
  • map2k1
  • MEK1
Epitope
pSer298
46
39
36
35
35
34
31
29
25
25
19
18
15
15
15
14
11
10
9
8
7
5
5
5
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
666
428
378
108
80
45
30
25
22
21
10
8
5
5
4
4
4
2
1
1
Host
Rabbit
539
129
5
3
2
1
Clonality
Monoclonal
Conjugate
This MAP2K1 antibody is un-conjugated
19
19
18
12
9
9
9
8
6
6
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Cultured Cells) (IF (cc)), Western Blotting (WB)
530
203
172
132
122
78
27
22
19
10
9
7
6
6
3
2
2
1
1
1
Options
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Catalog No. ABIN5557395
$ 402.49
Plus shipping costs $45.00

General

Antigen Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
Epitope pSer298
(46), (39), (36), (35), (35), (34), (31), (29), (25), (25), (19), (18), (15), (15), (15), (14), (11), (10), (9), (8), (7), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(666), (428), (378), (108), (80), (45), (30), (25), (22), (21), (10), (8), (5), (5), (4), (4), (4), (2), (1), (1)
Host Rabbit
(539), (129), (5), (3), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAP2K1 antibody is un-conjugated
(19), (19), (18), (12), (9), (9), (9), (8), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Cultured Cells) (IF (cc)), Western Blotting (WB)
(530), (203), (172), (132), (122), (78), (27), (22), (19), (10), (9), (7), (6), (6), (3), (2), (2), (1), (1), (1)
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Product Details anti-MAP2K1 Antibody

Target Details MAP2K1 Application Details Handling Images
Purification Purified by Protein A.
Immunogen Synthetic peptide derived from human MEK1 (S298), around 270-320aa (phospho S298).
Clone 20B10
Isotype IgG

Target Details MAP2K1

Product Details anti-MAP2K1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MEK1 (MAP2K1 Antibody Abstract)
Background

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

Synonyms: CFC3, MEK1, MKK1, MAPKK1, PRKMK1, Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1, MAP2K1

Gene ID 5604
UniProt Q02750
Pathways MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling Images back to top
Application Notes WB 1:1000-2000
IF(ICC) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

Images

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer298) antibody (ABIN5557395) Lane 1: Hela, Lane 2: NIH/3T3, Lane 3: A431 cell lysates probed with MEK1 (S298) (20B...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer298) antibody (ABIN5557395) 3T2 cells were stained with MEK1 (S298) (20B10 ) Monoclonal Antibody at [1:200] incu...