anti-Human MAP2K1 antibody for Immunohistochemistry

Recommended MAP2K1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
  • Mek1
  • CFC3
  • MAPKK1
  • MEK1
  • MKK1
  • PRKMK1
  • MEKK1
  • Prkmk1
  • mek-2
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
  • ATMEK1
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • NMAPKK
  • MEK
  • mitogen activated protein kinase kinase 1
  • mitogen-activated protein kinase kinase 1
  • mitogen-activated protein kinase kinase 1 L homeolog
  • MAP kinase/ ERK kinase 1
  • Map2k1
  • MAP2K1
  • map2k1.L
  • map2k1
  • MEK1
Epitope
pThr386
39
39
38
35
32
32
32
27
26
20
19
18
16
15
15
15
11
10
10
10
8
7
6
5
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
696
447
395
105
81
44
30
24
21
19
10
8
5
5
4
4
4
1
1
Host
Rabbit
568
128
5
3
2
1
Clonality
Polyclonal
Conjugate
This MAP2K1 antibody is un-conjugated
20
19
18
12
10
9
8
7
5
5
5
5
5
5
5
5
5
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
568
282
206
131
129
66
30
24
23
10
10
8
7
6
6
2
1
1
1
1
Supplier
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Catalog No. ABIN6270106
$ 450.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
21.748539 ABIN3181573 IHC WB Rabbit IgG Log in to see Polyclonal 0
19 ABIN1499381 IF IHC IHC (p) WB Mouse IgG1 Log in to see 3H3 0
19 ABIN1499380 IF IHC IHC (p) WB Mouse IgG2b Log in to see 4F1 0
19 ABIN1499378 IF IHC IHC (p) WB Mouse IgG2b Log in to see 3H5 0
19 ABIN1499385 IF IHC IHC (p) WB Mouse IgG1 Log in to see 7E2 0
19 ABIN1499394 IF IHC IHC (p) WB Mouse IgG2a Log in to see 2F12 0
19 ABIN1499383 IF IHC IHC (p) WB Mouse IgG2b Log in to see 2F1 0
19 ABIN1499388 IF IHC IHC (p) WB Mouse IgG2b Log in to see 8A6 0
19 ABIN1499389 IF IHC IHC (p) WB Mouse IgG1 Log in to see 1A10 0
19 ABIN1499391 IF IHC IHC (p) WB Mouse IgG1 Log in to see 3E6 0
19 ABIN1499376 IF IHC IHC (p) WB Mouse IgG2a Log in to see 2B2 0
19 ABIN1499377 IF IHC IHC (p) WB Mouse IgG2b Log in to see 2A9 0
19 ABIN1499392 IF IHC IHC (p) WB Mouse IgG2b Log in to see 2A1 0
19 ABIN1499393 IF IHC IHC (p) WB Mouse IgG2a Log in to see 4B1 0
19 ABIN1499379 IF IHC IHC (p) WB Mouse IgG2b Log in to see 4H8 0
19 ABIN1499374 IF IHC IHC (p) WB Mouse IgG2b Log in to see 2H3 0
18.992775 ABIN6270104 ELISA IHC WB Rabbit IgG pSer298 Log in to see Polyclonal 0
18.755764 ABIN3185523 ELISA IHC WB Rabbit IgG Thr38 Log in to see Polyclonal 0
17.255764 ABIN966559 IHC Rabbit pSer217 Log in to see 5
17.255764 ABIN966561 IHC Rabbit pThr291 Log in to see 4

General

Antigen Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
Epitope pThr386
(39), (39), (38), (35), (32), (32), (32), (27), (26), (20), (19), (18), (16), (15), (15), (15), (11), (10), (10), (10), (8), (7), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(696), (447), (395), (105), (81), (44), (30), (24), (21), (19), (10), (8), (5), (5), (4), (4), (4), (1), (1)
Host Rabbit
(568), (128), (5), (3), (2), (1)
Clonality
Polyclonal
Conjugate This MAP2K1 antibody is un-conjugated
(20), (19), (18), (12), (10), (9), (8), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
(568), (282), (206), (131), (129), (66), (30), (24), (23), (10), (10), (8), (7), (6), (6), (2), (1), (1), (1), (1)
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Product Details anti-MAP2K1 Antibody

Target Details MAP2K1 Application Details Handling Images
Specificity Phospho-MEK1 (Thr386) Antibody detects endogenous levels of MEK1 only when phosphorylated at Thr386
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human MEK1 around the phosphorylation site of Thr386
Isotype IgG
Plasmids, Primers & others

Target Details MAP2K1

Product Details anti-MAP2K1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MEK1 (MAP2K1 Antibody Abstract)
Background

Description: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

Gene: MAP2K1

Molecular Weight 45kDa
Gene ID 5604
UniProt Q02750
Pathways MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling Images back to top
Application Notes WB 1:500-1:2000, IHC 1:50-1:200
Restrictions For Research Use only

Handling

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pThr386) antibody (ABIN6270106) ABIN6270107 at 1/100 staining mouse heart tissue sections by IHC-P. The tissue was fo...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pThr386) antibody (ABIN6270106) ABIN6270107 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was f...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pThr386) antibody (ABIN6270106) ABIN6270107 at 1/100 staining rat heart tissue sections by IHC-P. The tissue was form...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pThr386) antibody (ABIN6270106) ABIN6270107 at 1/100 staining human gastric cancer tissue sections by IHC-P. The tiss...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pThr386) antibody (ABIN6270106) ABIN6270107 at 1/100 staining human liver tissue sections by IHC-P. The tissue was fo...
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pThr386) antibody (ABIN6270106) Western blot analysis of Phospho-MEK1 (Thr386) in lysates of NIH-3T3 , using Phospho-...