anti-Human MAP2K2 antibody for Flow Cytometry

Recommended MAP2K2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) Antibodies
  • CFC4
  • MAPKK2
  • MEK2
  • MKK2
  • PRKMK2
  • AA589381
  • MK2
  • Prkmk2
  • ATMKK2
  • F27B13.50
  • F27B13_50
  • MAP KINASE KINASE 1
  • MAP kinase kinase 2
  • MK1
  • zgc:172250
  • mitogen-activated protein kinase kinase 2
  • mitogen activated protein kinase kinase 2
  • MAP kinase kinase 2
  • mitogen-activated protein kinase kinase 2b
  • MAP2K2
  • Map2k2
  • MKK2
  • map2k2b
Reactivity
Human, Mouse (Murine), Rat (Rattus)
315
176
138
13
12
4
3
2
1
1
Host
Mouse
249
57
13
1
Clonality
Monoclonal
Conjugate
This MAP2K2 antibody is un-conjugated
16
15
15
7
7
7
2
2
2
2
2
2
2
2
2
2
2
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
271
120
118
50
47
39
27
18
15
13
12
11
4
1
Options
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Catalog No. ABIN2869479
$ 475.29
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.455378 ABIN5950421 FACS ICC IF IHC IHC (p) IP WB Rabbit IgG AA 1-100 Log in to see SD207-08 0
14.455378 ABIN1844273 ELISA FACS IF/ICC WB Mouse IgG1 Log in to see 0
14.455378 ABIN5682805 ELISA FACS ICC IF WB Mouse IgG1 Log in to see 7F5 0
14.455378 ABIN1724726 ICC FACS ELISA WB Mouse IgG1 Log in to see 7F5 1
14.455378 ABIN4333499 FACS IHC IHC (p) WB Mouse IgG1 Log in to see 8G6 0
14.455378 ABIN5582996 ELISA FACS IF WB Mouse IgG1 Log in to see 7F5 0
1 ABIN1499477 FACS IHC IHC (p) WB Mouse IgG1 Log in to see 8G6 0
1 ABIN1499476 FACS IHC IHC (p) WB Mouse IgG1 Log in to see 5F6 0
1 ABIN466779 ELISA FACS Mouse IgG1 Log in to see 7F5 0
1 ABIN2725467 FACS IHC WB Mouse IgG1 Log in to see 8G6 0
1 ABIN2725466 FACS IHC WB Mouse IgG1 Log in to see 5F6 0
1 ABIN4901927 ELISA FACS IF WB Mouse IgG1 Log in to see 7F5 0
1 ABIN6249135 FACS IHC WB Mouse IgG1 Log in to see OTI5F6 0
1 ABIN6249136 FACS IHC WB Mouse IgG1 Log in to see OTI8G6 0
1 ABIN2314865 IC FACS IP IHC WB Rabbit IgG N-Term Log in to see 7i37 0
1 ABIN2306790 FACS ELISA WB Mouse IgG1 Log in to see 13B493 0
1 ABIN2146178 FACS IF ELISA WB Mouse IgG1 Log in to see 7F5 0
1 ABIN1577723 FACS IHC ELISA WB Mouse Log in to see 0

General

Antigen Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(315), (176), (138), (13), (12), (4), (3), (2), (1), (1)
Host Mouse
(249), (57), (13), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAP2K2 antibody is un-conjugated
(16), (15), (15), (7), (7), (7), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
(271), (120), (118), (50), (47), (39), (27), (18), (15), (13), (12), (11), (4), (1)
Pubmed 3 references available
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Product Details anti-MAP2K2 Antibody

Target Details MAP2K2 Application Details Handling References for anti-MAP2K2 antibody (ABIN2869479) Images
Purpose Purified Mouse Monoclonal Antibody
Characteristics Synonyms: Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, 2.7.12.2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK 2, MAP2K2, MEK2, MKK2, PRKMK2
Purification Ascitic fluid
Immunogen

Immunogen: Purified recombinant fragment of human MAP2K2 expressed in E. Coli.

Antigen Type: Recombinant Protein

Clone 7F5
Isotype IgG1
Plasmids, Primers & others

Target Details MAP2K2

Product Details anti-MAP2K2 Antibody Application Details Handling References for anti-MAP2K2 antibody (ABIN2869479) Images back to top
Antigen
Alternative Name MAP2K2 (MAP2K2 Antibody Abstract)
Background

Other Names: Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, 2.7.12.2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK 2, MAP2K2, MEK2, MKK2, PRKMK2

Description: MAP2K2, also called MEK2, it is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax.

Molecular Weight 44424
Gene ID 5605
UniProt P36507
Pathways MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Handling References for anti-MAP2K2 antibody (ABIN2869479) Images back to top
Application Notes Western Bloting: = 1:500 - 2000. Immunofluorescence: = 1:200 - 1000. Flow cytometry: = 1:200 - 400.
Restrictions For Research Use only

Handling

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details References for anti-MAP2K2 antibody (ABIN2869479) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-MAP2K2 antibody (ABIN2869479)

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details Handling Images back to top
Background publications

Tanimura, Chatani, Hoshino, Sato, Watanabe, Kataoka, Nakamura, Kohno: "Activation of the 41/43 kDa mitogen-activated protein kinase signaling pathway is required for hepatocyte growth factor-induced cell scattering." in: Oncogene, Vol. 17, Issue 1, pp. 57-65, 1998

Moriguchi, Gotoh, Nishida: "Activation of two isoforms of mitogen-activated protein kinase kinase in response to epidermal growth factor and nerve growth factor." in: European journal of biochemistry / FEBS, Vol. 234, Issue 1, pp. 32-8, 1996

Charest, Mordret, Harder, Jirik, Pelech: "Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4679-90, 1993

Images

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details Handling References for anti-MAP2K2 antibody (ABIN2869479) back to top
Supplier Images
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) antibody (ABIN2869479) Figure 1: Western blot analysis using MAP2K2 mouse mAb against PC-12 (1), Jurkat (2),...
Immunofluorescence (IF) image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) antibody (ABIN2869479) Figure 2: Immunofluorescence analysis of Hela cells using anti-MAP2K2 mAb (green). Re...
Flow Cytometry (FACS) image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) antibody (ABIN2869479) Figure 3: Flow cytometric analysis of Hela cells using anti-MAP2K2 mAb (green) and ne...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) antibody (ABIN2869479) Figure 3: Immunohistochemical analysis of paraffin-embedded human Liver tissues using...