anti-Human MAP2K2 antibody for Immunofluorescence

Recommended MAP2K2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) Antibodies
  • Prkmk2
  • CFC4
  • MAPKK2
  • MEK2
  • MKK2
  • PRKMK2
  • AA589381
  • MK2
  • ATMKK2
  • F27B13.50
  • F27B13_50
  • MAP KINASE KINASE 1
  • MAP kinase kinase 2
  • MK1
  • mitogen activated protein kinase kinase 2
  • mitogen-activated protein kinase kinase 2
  • MAP kinase kinase 2
  • Map2k2
  • MAP2K2
  • MKK2
Epitope
Center
55
38
27
20
17
16
14
12
10
10
10
7
5
4
4
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
351
174
137
12
11
4
3
2
1
1
Host
Rabbit
244
98
13
1
Clonality
Polyclonal
Conjugate
This MAP2K2 antibody is un-conjugated
19
18
15
7
7
7
5
5
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
308
118
117
51
46
38
27
18
15
13
12
11
4
1
Options
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Catalog No. ABIN2855313
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.13544 ABIN519254 IF IP WB Rabbit AA 1-400, full length Log in to see Polyclonal 0
12.13544 ABIN1870344 IF IHC WB Rabbit IgG pThr394 Log in to see Polyclonal 0
12.13544 ABIN1882178 IF WB Rabbit AA 1-30, N-Term Log in to see Polyclonal 5
12.13544 ABIN519255 IF WB Rabbit AA 1-400, full length Log in to see Polyclonal 0
12.13544 ABIN948323 IF ELISA WB Mouse IgG2b kappa AA 1-360, partial Log in to see 8D10 0
12.13544 ABIN4333500 ELISA FACS ICC IF WB Mouse IgG1 Log in to see 7F5 0
12.13544 ABIN4333503 ICC IF IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
12.13544 ABIN4333502 ICC IF IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
12.13544 ABIN4333504 IF IHC WB Mouse IgG2a Log in to see 1A-2 0
12.13544 ABIN1108139 EIA IF WB Mouse IgG1 Log in to see 7F5 1
12.13544 ABIN4333501 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal 0
12.13544 ABIN2424775 IF IHC WB Rabbit IgG pThr394 Log in to see Polyclonal 0
12.13544 ABIN3019566 IF IHC WB Rabbit pThr394 Log in to see Polyclonal 0
1 ABIN214275 IF IHC IHC (p) WB Rabbit IgG pThr394 Log in to see Polyclonal 0
1 ABIN392485 IF IHC (p) WB Rabbit Ig Fraction AA 262-292, Center Log in to see Polyclonal 0
1 ABIN1499478 IF WB Mouse IgG1 Log in to see 5B4 0
1 ABIN196622 IF IHC (p) WB Rabbit pThr394 Log in to see Polyclonal 2
1 ABIN5534508 IF IHC (p) WB Rabbit Ig Fraction AA 262-292 Log in to see Polyclonal 0
1 ABIN361888 IF IHC WB Rabbit IgG pThr394 Log in to see Polyclonal 4
1 ABIN5534506 IF IHC (p) WB Rabbit Ig Fraction AA 372-400 Log in to see Polyclonal 0

General

Antigen Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) Antibodies
Epitope Center
(55), (38), (27), (20), (17), (16), (14), (12), (10), (10), (10), (7), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(351), (174), (137), (12), (11), (4), (3), (2), (1), (1)
Host Rabbit
(244), (98), (13), (1)
Clonality
Polyclonal
Conjugate This MAP2K2 antibody is un-conjugated
(19), (18), (15), (7), (7), (7), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(308), (118), (117), (51), (46), (38), (27), (18), (15), (13), (12), (11), (4), (1)
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Product Details anti-MAP2K2 Antibody

Target Details MAP2K2 Application Details Handling Images
Cross-Reactivity Chicken, Dog (Canine), Rat (Rattus), Cow (Bovine)
Cross-Reactivity (Details) Chicken (91 %), Dog (93 %), Rat (92 %), Bovine (93 %)
Characteristics Rabbit Polyclonal antibody to MEK2 (mitogen-activated protein kinase kinase 2)
MEK2 antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human MEK2. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details MAP2K2

Product Details anti-MAP2K2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MEK2 (MAP2K2 Antibody Abstract)
Background The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene.

Molecular Weight 44 kDa
Gene ID 5605
Pathways MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , Jurkat , Raji

Restrictions For Research Use only

Handling

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details Images back to top
Format Liquid
Concentration 0.97 mg/mL
Buffer 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (Center) antibody (ABIN2855313) ICC/IF Image Immunofluorescence analysis of methanol-fixed HeLa, using MEK2, antibody...
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (Center) antibody (ABIN2855313) WB Image Sample (30 ug of whole cell lysate) A: A431 , 10% SDS PAGE antibody diluted ...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (Center) antibody (ABIN2855313) IHC-P Image Immunohistochemical analysis of paraffin-embedded human colon carcinoma, ...