anti-Mouse (Murine) MAP2K2 antibody for Immunofluorescence (Cultured Cells)

Recommended MAP2K2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) Antibodies
  • Prkmk2
  • CFC4
  • MAPKK2
  • MEK2
  • MKK2
  • PRKMK2
  • AA589381
  • MK2
  • ATMKK2
  • F27B13.50
  • F27B13_50
  • MAP KINASE KINASE 1
  • MAP kinase kinase 2
  • MK1
  • mitogen activated protein kinase kinase 2
  • mitogen-activated protein kinase kinase 2
  • MAP kinase kinase 2
  • Map2k2
  • MAP2K2
  • MKK2
Epitope
AA 1-50
56
38
27
20
16
16
14
12
10
10
10
7
5
4
4
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
351
173
136
12
11
4
3
2
1
1
Host
Rabbit
244
98
13
1
Clonality
Polyclonal
Conjugate
This MAP2K2 antibody is un-conjugated
19
18
15
7
7
7
5
5
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
308
118
117
51
47
38
26
18
15
13
12
11
4
1
Options
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Available images

Western Blotting validation image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-50) antibody (ABIN726500)
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-50) antibody (ABIN726500)
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-50) antibody (ABIN726500)
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-50) antibody (ABIN726500)
Catalog No. ABIN726500
$ 306.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN906102 IF (cc) IF (p) Alexa Fluor 555 Rabbit IgG AA 1-50 Log in to see Polyclonal 0
1 ABIN906105 IF (cc) IF (p) Alexa Fluor 647 Rabbit IgG AA 1-50 Log in to see Polyclonal 0
1 ABIN906106 IF (cc) IF (p) Alexa Fluor 488 Rabbit IgG AA 1-50 Log in to see Polyclonal 0
1 ABIN906104 IF (cc) IF (p) Alexa Fluor 350 Rabbit IgG AA 1-50 Log in to see Polyclonal 0
1 ABIN726507 IF (cc) IF (p) FITC Rabbit IgG AA 1-50 Log in to see Polyclonal 0
1 ABIN726504 IF (cc) IF (p) Cy5 Rabbit IgG AA 1-50 Log in to see Polyclonal 0
1 ABIN726503 IF (cc) IF (p) Cy3 Rabbit IgG AA 1-50 Log in to see Polyclonal 0
1 ABIN726506 IF (cc) IF (p) Cy7 Rabbit IgG AA 1-50 Log in to see Polyclonal 0
1 ABIN726505 IF (cc) IF (p) Cy5.5 Rabbit IgG AA 1-50 Log in to see Polyclonal 0
1 ABIN2808137 IF (cc) IF (p) Alexa Fluor 594 Rabbit IgG AA 1-50 Log in to see Polyclonal 0
1 ABIN5006727 IF (cc) IF (p) Alexa Fluor 750 Rabbit IgG AA 1-50 Log in to see Polyclonal 0
1 ABIN5006726 IF (cc) IF (p) Alexa Fluor 680 Rabbit IgG AA 1-50 Log in to see Polyclonal 0

General

Antigen Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) Antibodies
Epitope AA 1-50
(56), (38), (27), (20), (16), (16), (14), (12), (10), (10), (10), (7), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(351), (173), (136), (12), (11), (4), (3), (2), (1), (1)
Host Rabbit
(244), (98), (13), (1)
Clonality
Polyclonal
Conjugate This MAP2K2 antibody is un-conjugated
(19), (18), (15), (7), (7), (7), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(308), (118), (117), (51), (47), (38), (26), (18), (15), (13), (12), (11), (4), (1)
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Product Details anti-MAP2K2 Antibody

Target Details MAP2K2 Application Details Handling Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human MAPKK2/MEK2
Isotype IgG
Plasmids, Primers & others

Target Details MAP2K2

Product Details anti-MAP2K2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MEK2 (MAP2K2 Antibody Abstract)
Background

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).

Subcellular location: Intracellular

Synonyms: CFC4, MEK2, MKK2, MAPKK2, PRKMK2, Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK 2, MAP2K2
Gene ID 5605
UniProt P36507
Pathways MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Handling Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

Images

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-50) antibody (ABIN726500) Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti MEK2/MAPK...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-50) antibody (ABIN726500) HT-55 cells were stained with bs-0223R Rabbit Anti-MEK2 Polyclonal Antibody at 1:250....
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-50) antibody (ABIN726500) Images provided by the Independent Validation Program, badge number 029748. Lane one:...
'Independent Validation' Badge
Lot Number 110511
Method validated Western Blotting
Positive Control Hela cell extract
Negative Control c6/36 Mosquito cell extract (non-reactive species)
Notes A single positive band at the correct molecular weight was detected in positive control HeLa cell extract. Several bands were observed in the non-reactive species negative control at approximately the same molecular weight as MAP2K2. These bands may constitute cross-reactivity of the target antigen, or they may be non-specific bands.
Primary Antibody
  • Antigen: Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (1:150 dilution)
  • Catalog number: ABIN726500
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  • Supplier catalog number: Log in to see
  • Lot number: 110511
Secondary Antibody
  • Antibody: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:20,000 dilution)
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  • Catalog number: Log in to see
  • Lot number: L170-6515
Controls
  • Positive control: HeLa cell extract
  • Negative control: c6/36 Mosquito cell extract
Protocol
  • 1. Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
  • 2. 46 μg of boiled extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
  • 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
  • 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • 5. The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) non-fat dry milk at room temperature for 1 h.
  • 6. The membrane was rinsed with TBST once.
  • 7. The membrane was immersed with the protein side up in the primary antibody solution (anti-MAP2K2; 1:150) in TBST containing 5% (W/V) non-fat dry milk and incubated for 16 h at 4°C.
  • 8. The membrane was rinsed in TBST thrice for 5 min each.
  • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) non-fat dry milk and incubated for 1 h at room temperature (~26°C) with gentle agitation.
  • 10. The membrane was rinsed thrice TBST thrice for 5 min each.
  • 11. The membrane was rinsed in TBS twice for 30 s each.
  • 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
  • 13. The membrane was rinsed three times TBST.
  • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
  • 15. The membrane was washed in TBST 2 times for 10 min each.
  • 16. Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).
Experimental Notes - No challenges noted.
Validation Images
Western Blotting validation image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-50) antibody (ABIN726500) Figure 1. Western blot of lysates from HeLa cells (Lane 1) and c6/36 cells (Lane 2) p...