anti-Mouse (Murine) MAP2K3 antibody for Enzyme Immunoassay

Recommended MAP2K3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) Antibodies
  • Mkk3
  • MAPKK3
  • MEK3
  • MKK3
  • PRKMK3
  • SAPKK-2
  • SAPKK2
  • AW212142
  • Prkmk3
  • mMKK3b
  • map2k3
  • zMKK3
  • MAP kinase activator XMEK3
  • mitogen activated protein kinase kinase 3
  • mitogen-activated protein kinase kinase 3
  • mitogen-activated protein kinase kinase 6
  • map2k3
  • Map2k3
  • MAP2K3
  • map2k6
Reactivity
Dog (Canine), Human, Mouse (Murine)
314
156
146
56
40
7
6
6
5
3
2
1
1
1
Host
Mouse
210
104
Clonality
Monoclonal
Conjugate
This MAP2K3 antibody is un-conjugated
8
8
7
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
273
105
100
81
67
26
22
14
11
3
2
2
1
1
Supplier
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Available images

Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) antibody (ABIN126840)
Catalog No. ABIN126840
$ 555.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN318070 EIA IHC (p) WB Rabbit pSer189 Log in to see Polyclonal 0

General

Antigen Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) Antibodies
Reactivity Dog (Canine), Human, Mouse (Murine)
(314), (156), (146), (56), (40), (7), (6), (6), (5), (3), (2), (1), (1), (1)
Host Mouse
(210), (104)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAP2K3 antibody is un-conjugated
(8), (8), (7), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(273), (105), (100), (81), (67), (26), (22), (14), (11), (3), (2), (2), (1), (1)
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Product Details anti-MAP2K3 Antibody

Target Details MAP2K3 Application Details Handling Images
Specificity This antibody specifically recognizes the N-terminus of MKK3.
Characteristics Synonyms: MAPKK 3, MEK3, MKK3, MAP kinase kinase 3, Dual specificity mitogen-activated proteinkinase kinase 3
Purification Size exclusion chromatography
Components incl. positive Control
Immunogen Synthetic peptide conjugated to KLH
Clone 5F7
Isotype IgG1

Target Details MAP2K3

Product Details anti-MAP2K3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAP2K3 (MAP2K3 Antibody Abstract)
Background MKK3 activates p38 MAP kinase by phosphorylating a Thr and Tyr residue in the activation loop. MKK3 does not phosphorylate and activate the other major MAP kinases, MAPK1/2 (erk1/2) or SAPK/JNK. Cellular stress and inflammatory cytokines activate MKK3 and lead to phosphorylation of Ser 189 and Thr 193.Synonyms: Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MEK3, MKK3
Gene ID 5606
UniProt P46734
Pathways MAPK Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2

Application Details

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Handling Images back to top
Application Notes Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated A431 cells. ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C/-80 °C
Storage Comment Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) antibody (ABIN126840) anti-Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) antibody