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|Antigen||Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) Antibodies|
|Epitope||AA 311-347, C-Term Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAP2K3 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|2 references available|
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Product Details anti-MAP2K3 AntibodyTarget Details MAP2K3 Application Details Handling References for anti-MAP2K3 antibody (ABIN3043874) Images
|Purpose||Rabbit IgG polyclonal antibody for Dual specificity mitogen-activated protein kinase kinase 3(MAP kinase kinase 3/MAPKK 3)(MAP2K3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.|
|Sequence||AERMSYLELM EHPFFTLHKT KKTDIAAFVK EILGEDS|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Dual specificity mitogen-activated protein kinase kinase 3(MAP kinase kinase 3/MAPKK 3)(MAP2K3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: mitogen-activated protein kinase kinase 3
Protein Name: Dual specificity mitogen-activated protein kinase kinase 3(MAP kinase kinase 3/MAPKK 3)
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human MEK3 (311-347aa AERMSYLELMEHPFFTLHKTKKTDIAAFVKEILGEDS), identical to the related mouse sequence.|
|Plasmids, Primers & others|
Target Details MAP2K3Product Details anti-MAP2K3 Antibody Application Details Handling References for anti-MAP2K3 antibody (ABIN3043874) Images back to top
|Alternative Name||MAP2K3 (MAP2K3 Antibody Abstract)|
Dual specificity mitogen-activated protein kinase kinase 3 is an enzyme that in humans is encoded by the MAP2K3 gene. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. And this kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. Rampoldi et al. (1997) localized the MAP2K3 gene to 17q11.2.
Synonyms: AW212142 antibody|Dual specificity mitogen activated protein kinase kinase 3 antibody|Dual specificity mitogen-activated protein kinase kinase 3 antibody|ERK kinase 3 antibody|MAP kinase kinase 3 antibody|MAP2K 3 antibody|map2k3 antibody|MAPK ERK kinase 3 antibody|MAPK kinase 3 antibody|MAPK/ERK kinase 3 antibody|MAPKK 3 antibody|MAPKK3 antibody|MEK 3 antibody|MEK3 antibody|Mitogen activated protein kinase kinase 3 antibody|MKK 3 antibody|MKK3 antibody| mMKK 3b antibody| mMKK3b antibody|MP2K3_HUMAN antibody|MPK 3 antibody|PRKMK 3 antibody|PRKMK3 antibody|Protein kinase mitogen activated kinase 3 antibody|protein kinase, mitogen-activated, kinase 3 antibody|SAPK kinase 2 antibody|SAPKK 2 antibody|SAPKK2 antibody|SKK2 antibody|Stress activated protein kinase kinase 2 antibody| zMKK 3 antibody
|Pathways||MAPK Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2|
Application DetailsProduct Details anti-MAP2K3 Antibody Target Details MAP2K3 Handling References for anti-MAP2K3 antibody (ABIN3043874) Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details References for anti-MAP2K3 antibody (ABIN3043874) Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
References for anti-MAP2K3 antibody (ABIN3043874)Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details Handling Images back to top
|Product cited in:||
Cheng, Wang, Wang, Wang, Du, Lou: "Silencing Ras-Related C3 Botulinum Toxin Substrate 1 Inhibits Growth and Migration of Hypopharyngeal Squamous Cell Carcinoma via the P38 Mitogen-Activated Protein Kinase Signaling Pathway." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 768-781, 2018
Xu, Zhang, Wei, Jia, Ge, Zhang, Liu: "MicroRNA-21 promotes hepatocellular carcinoma HepG2 cell proliferation through repression of mitogen-activated protein kinase-kinase 3." in: BMC cancer, Vol. 13, pp. 469, 2013
ImagesProduct Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details Handling References for anti-MAP2K3 antibody (ABIN3043874) back to top