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|Antigen||Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) Antibodies|
|Epitope||pSer311, pThr315 Alternatives|
|Reactivity||Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Non-Human Primate, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio) Alternatives|
|Conjugate||This MAP2K5 antibody is un-conjugated Alternatives|
Western Blotting (WB)
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Product Details anti-MAP2K5 AntibodyTarget Details MAP2K5 Application Details Handling Images
|Specificity||This antibody is specific for the ~49k MEK5 protein phosphorylated at Ser311, Thr315. Immunolabeling of the MEK5 band is blocked by preadsorption with the phospho-peptide used as antigen but not be the corresponding dephospho-peptide.|
Species reactivity (expected):Mouse, Human, Chicken, Bovine, Canine, non-Human Primates, Xenopus, and Zebrafish.
Species reactivity (tested):Rat.
|Purification||Sequential Chromatography on phospho- and dephosphopeptide affinity columns.|
|Immunogen||Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser311, Thr315 of MEK5.|
Target Details MAP2K5Product Details anti-MAP2K5 Antibody Application Details Handling Images back to top
|Alternative Name||MAP2K5 (MAP2K5 Antibody Abstract)|
|Background||MEK5 (also known as MKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. MEK5 has been shown to specifically activate ERK5 (Zhou et al., 1995) whereas MEK5 itself is regulated by MEKK3 (Chao et al., 1999). An important link between MEK5 and metastatic prostrate cancer has been demonstrated (Mehta et.al., 2003). Dual phosphorylation of Ser311 and Thr315 have been implicated in cell proliferation (Cameron et al., 2004).Synonyms: Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MEK5, MKK5|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-MAP2K5 Antibody Target Details MAP2K5 Handling Images back to top
Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP2K5 Antibody Target Details MAP2K5 Application Details Images back to top
|Buffer||10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody undiluted (in aliquots) at-20 °C.|