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|Antigen||Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) Antibodies|
|Epitope||pSer311, pThr315 Alternatives|
|Reactivity||Cow (Bovine), Dog (Canine), Chicken, Human, Monkey, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio) Alternatives|
|Conjugate||This MAP2K5 antibody is un-conjugated Alternatives|
Western Blotting (WB)
|1 reference available|
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Product Details anti-MAP2K5 AntibodyTarget Details MAP2K5 Application Details Handling References for anti-MAP2K5 antibody (ABIN318850) Images
|Specificity||This antibody specifically recognises 49 kDa MEK5, also known as MAP2K5, when phosphorylated at Ser311 and Thr315. Activation of MEK5 itself is regulated by upstream MAP3K3/MEKK3, and phosphorylation of MEK5 at both the Ser311 and Thr315 sites has been implicated in cell proliferation.|
|Immunogen||Synthetic phosphopeptide corresponding to an amino acid sequence within MEK5 , which includes phoshorylated Ser311 and Thr315.|
Target Details MAP2K5Product Details anti-MAP2K5 Antibody Application Details Handling References for anti-MAP2K5 antibody (ABIN318850) Images back to top
|Alternative Name||MAP2K5 (MAP2K5 Antibody Abstract)|
|Background||MEK5 is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family, which acts as a key regulator in a mitogen-activated protein (MAP) kinase cascade, and hence growth factor-induced cellular stimulation. MEK5 exists in naturally occurring MEK5alpha and MEK5beta splice variants, the former of which is known to specifically activate/regulate MAPK7/ERK5/BMK1, whilst the latter may act as a suppressor of MAPK7 activation.Synonyms: Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MEK5, MKK5|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-MAP2K5 Antibody Target Details MAP2K5 Handling References for anti-MAP2K5 antibody (ABIN318850) Images back to top
Western Blot: 1: 1000, detects a band of approximately 49 kDa in rat testes cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP2K5 Antibody Target Details MAP2K5 Application Details References for anti-MAP2K5 antibody (ABIN318850) Images back to top
|Buffer||10 mM Hepes pH 7.5 containing 0.09 % Sodium Azide (NaN3), 0.01 % Bovine Serum Albumin, 50 % Glycerol|
|Precaution of Use||This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-MAP2K5 antibody (ABIN318850)Product Details anti-MAP2K5 Antibody Target Details MAP2K5 Application Details Handling Images back to top
Cameron, Abe, Malik, Che, Yang: "Differential role of MEK5alpha and MEK5beta in BMK1/ERK5 activation." in: The Journal of biological chemistry, Vol. 279, Issue 2, pp. 1506-12, 2004