anti-Mouse (Murine) MAP3K5 antibody for ELISA

Recommended MAP3K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
  • ASK1
  • MAPKKK5
  • MEKK5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
  • 7420452D20Rik
  • ASK
  • Mekk5
  • Ask1
  • RGD1306565
  • mitogen-activated protein kinase kinase kinase 5
  • MAP3K5
  • MAPKKK5
  • Map3k5
  • map3k5
Epitope
AA 932-981, pSer966
66
52
30
23
19
18
15
15
15
10
10
9
7
6
6
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
407
179
99
5
1
1
Host
Rabbit
385
28
Clonality
Polyclonal
Conjugate
This MAP3K5 antibody is un-conjugated
11
11
10
5
5
5
5
5
5
5
5
5
5
5
4
4
4
2
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
320
177
138
84
65
51
25
14
12
11
8
4
2
1
1
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Catalog No. ABIN1531773
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1532802 IHC ELISA WB Rabbit IgG AA 932-981 Log in to see Polyclonal 2
1 ABIN2888359 ELISA IHC IHC (p) WB Rabbit IgG pSer966 Log in to see Polyclonal 0
1 ABIN2888947 ELISA IHC WB Rabbit IgG AA 932-981 Log in to see Polyclonal 0
1 ABIN461897 ELISA ICC IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN461898 ELISA IHC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN6255571 ELISA ICC IF IHC WB Rabbit IgG pSer966 Log in to see Polyclonal 0
1 ABIN6271358 ELISA ICC IF WB Rabbit IgG pThr838 Log in to see Polyclonal 0
1 ABIN5958918 ELISA IHC (p) WB Rabbit IgG pSer966 Log in to see Polyclonal 0
1 ABIN3181921 ELISA IHC WB Rabbit IgG pSer966 Log in to see Polyclonal 0
1 ABIN3183403 ELISA IHC WB Rabbit IgG Ser897 Log in to see Polyclonal 0
1 ABIN6260079 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6260078 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6260080 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1736719 IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1736718 ICC IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1031241 ELISA WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN600478 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1030199 ICC ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN5958916 ELISA IHC (p) WB Rabbit IgG Ser966 Log in to see Polyclonal 0
1 ABIN4281878 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
Epitope AA 932-981, pSer966
(66), (52), (30), (23), (19), (18), (15), (15), (15), (10), (10), (9), (7), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(407), (179), (99), (5), (1), (1)
Host Rabbit
(385), (28)
Clonality
Polyclonal
Conjugate This MAP3K5 antibody is un-conjugated
(11), (11), (10), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (2)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(320), (177), (138), (84), (65), (51), (25), (14), (12), (11), (8), (4), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-MAP3K5 Antibody

Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 antibody (ABIN1531773) Images
Specificity

ASK1 (Phospho-Ser966) Antibody detects endogenous levels of ASK1 only when phosphorylated at Ser966.

PhosphorylationH:S966 M:S973 R:S973

Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human ASK1 around the phosphorylation site of Ser966.
Isotype IgG
Plasmids, Primers & others

Target Details MAP3K5

Product Details anti-MAP3K5 Antibody Application Details Handling References for anti-MAP3K5 antibody (ABIN1531773) Images back to top
Antigen
Alternative Name ASK1 (MAP3K5 Antibody Abstract)
Background

Synonyms: Apoptosis signal-regulating kinase 1, ASK-1, kinase ASK1, M3K5, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5, Mitogen-activated protein kinase kinase kinase 5

NCBI Gene Symbol: MAP3K5

Molecular Weight 154 kDa
Gene ID 4217
OMIM 602448
UniProt Q99683
Pathways MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded protein response

Application Details

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling References for anti-MAP3K5 antibody (ABIN1531773) Images back to top
Application Notes WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Comment

Unigene-Number: Hs.186486 (NCBI Gene Symbol: MAP3K5)

Restrictions For Research Use only

Handling

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details References for anti-MAP3K5 antibody (ABIN1531773) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20°C for at least 1 year.
Expiry Date 12 months

References for anti-MAP3K5 antibody (ABIN1531773)

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling Images back to top
Background publications

Mungall, Palmer, Sims, Edwards, Ashurst, Wilming, Jones, Horton, Hunt, Scott, Gilbert, Clamp, Bethel, Milne, Ainscough, Almeida, Ambrose, Andrews, Ashwell, Babbage, Bagguley, Bailey, Banerjee, Barker et al.: "The DNA sequence and analysis of human chromosome 6. ..." in: Nature, Vol. 425, Issue 6960, pp. 805-11, 2003

Ichijo, Nishida, Irie, ten Dijke, Saitoh, Moriguchi, Takagi, Matsumoto, Miyazono, Gotoh: "Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways." in: Science (New York, N.Y.), Vol. 275, Issue 5296, pp. 90-4, 1997

Images

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 antibody (ABIN1531773) back to top
Supplier Images
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 932-981), (pSer966) antibody (ABIN1531773) Western blot analysis of extracts from 293 cells treated with TNF(20ng/ml)+calyculinA...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 932-981), (pSer966) antibody (ABIN1531773) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ASK1...