anti-Hamster MAPK3 antibody for Immunofluorescence

Recommended MAPK3 Antibody

Antigen
Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
  • ERK-1
  • ERK1
  • ERT2
  • HS44KDAP
  • HUMKER1A
  • P44ERK1
  • P44MAPK
  • PRKM3
  • p44-ERK1
  • p44-MAPK
  • Erk-1
  • Erk1
  • Ert2
  • Esrk1
  • Mnk1
  • Mtap2k
  • Prkm3
  • p44
  • p44erk1
  • p44mapk
  • fi06b09
  • wu:fi06b09
  • zERK1
  • Tb08.10J17.940
  • MAPK1
  • MNK1
  • ATMAPK3
  • ATMPK3
  • T6D9.4
  • mitogen-activated protein kinase 3
  • mitogen-activated protein kinase 3
  • mitogen activated protein kinase 3
  • mitogen-activated serine/threonine-protein kinase
  • MAPK3
  • Mapk3
  • mapk3
  • Tc00.1047053509475.10
  • Tb927.8.3550
  • CEK1
  • MPK3
Epitope
Tyr204, pThr202
74
48
39
35
23
18
18
16
15
12
11
10
9
9
9
8
7
6
5
4
4
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Hamster, Human, Mouse (Murine), Rat (Rattus)
535
299
279
51
41
38
38
24
19
18
16
10
10
9
9
7
7
4
3
2
2
1
1
1
1
Host
Mouse
423
115
19
6
1
Clonality
Monoclonal
Conjugate
This MAPK3 antibody is un-conjugated
35
26
25
15
14
14
9
9
9
9
9
9
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
424
198
196
87
72
62
55
39
36
35
15
13
8
4
3
2
1
1
1
Options
Catalog No. ABIN220487
$ 859.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
1 ABIN220486 ICC FACS IF IP ELISA WB Rabbit IgG Tyr204, pThr202 Polyclonal 0

General

Antigen Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
Epitope Tyr204, pThr202
(74), (48), (39), (35), (23), (18), (18), (16), (15), (12), (11), (10), (9), (9), (9), (8), (7), (6), (5), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Hamster, Human, Mouse (Murine), Rat (Rattus)
(535), (299), (279), (51), (41), (38), (38), (24), (19), (18), (16), (10), (10), (9), (9), (7), (7), (4), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(423), (115), (19), (6), (1)
Clonality
Monoclonal
Conjugate This MAPK3 antibody is un-conjugated
(35), (26), (25), (15), (14), (14), (9), (9), (9), (9), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(424), (198), (196), (87), (72), (62), (55), (39), (36), (35), (15), (13), (8), (4), (3), (2), (1), (1), (1)
Supplier

Product Details anti-MAPK3 Antibody

Target Details MAPK3 Application Details Handling Images
Specificity Detects endogenous levels of human p44 and p42 MAP kinase (Erk1 and Erk2) dually phosphorylated at threonine 202 and tyrosine 204. Does not cross-react with the corresponding phosphorylated residues of either SAPK/JNK or p38 MAP kinase. Species cross-reactivity: Hamster, mouse, rat and zebrafish.
Purification Ascites
Immunogen Synthetic phosphopeptide (KLH coupled) corresponding to residues around Thr202/Tyr204 of human p44 MAP kinase.

Type of Immunogen: Synthetic phospho-peptide
Isotype IgG1
Plasmids, Primers & others

Target Details MAPK3

Product Details anti-MAPK3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAPK3 / ERK1 (MAPK3 Antibody Abstract)
Background Name/Gene ID: MAPK3
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK3, ERK-1, ERT2, HUMKER1A, HS44KDAP, MAP kinase 1, MAP kinase isoform p44, MAPK 1, MAPK 3, p44, p44MAPK, p44-MAPK, p44ERK1, Insulin-stimulated MAP2 kinase, MAP kinase 3, p44-ERK1, ERK1, PRKM3
Gene ID 5595
UniProt P27361
Pathways MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals

Application Details

Product Details anti-MAPK3 Antibody Target Details MAPK3 Handling Images back to top
Application Notes Approved: ELISA, Flo (1:1000), ICC (1:200), IF (1:200), IP (1:500), WB (1:2000)

Usage: Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry. Western Blot: 1:2000, incubate membrane with diluted antibody in 5 % nonfat dry milk, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:500. Immunofluorescence: 1:200. Immunocytochemistry: 1:200. Flow Cytometry: 1:1000.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol, 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.