anti-Human MAPK3 antibody for Immunoprecipitation

Recommended MAPK3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
  • ERK-1
  • ERK1
  • ERT2
  • HS44KDAP
  • HUMKER1A
  • P44ERK1
  • P44MAPK
  • PRKM3
  • p44-ERK1
  • p44-MAPK
  • Erk-1
  • Erk1
  • Ert2
  • Esrk1
  • Mnk1
  • Mtap2k
  • Prkm3
  • p44
  • p44erk1
  • p44mapk
  • fi06b09
  • wu:fi06b09
  • zERK1
  • Tb08.10J17.940
  • MAPK1
  • MNK1
  • ATMAPK3
  • ATMPK3
  • T6D9.4
  • mitogen-activated protein kinase 3
  • mitogen-activated protein kinase 3
  • mitogen activated protein kinase 3
  • mitogen-activated serine/threonine-protein kinase
  • MAPK3
  • Mapk3
  • mapk3
  • Tc00.1047053509475.10
  • Tb927.8.3550
  • CEK1
  • MPK3
Epitope
AA 279-379
65
55
39
38
24
18
16
16
15
14
13
11
11
10
8
7
5
5
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
521
295
269
51
42
38
38
24
21
21
20
10
10
9
9
8
7
4
3
2
2
2
1
1
1
1
Host
Mouse
401
124
20
6
1
Clonality
Monoclonal
Conjugate
This MAPK3 antibody is un-conjugated
27
25
23
14
13
13
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
448
209
201
104
85
70
58
52
39
35
15
13
8
4
3
2
1
1
1
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Catalog No. ABIN393694
$ 461.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
28.20631 ABIN562406 IF IHC (p) IP ELISA WB Mouse IgG2a kappa AA 279-379, partial Log in to see 3C9 0
19 ABIN2928446 IF/ICC IHC IP WB Rabbit AA 75-312 Log in to see Polyclonal 0
16.20631 ABIN5577504 IP WB Mouse IgG1 Log in to see E32 0
16.20631 ABIN5577503 IP WB Mouse IgG1 Log in to see E19 0
16 ABIN5997046 IF/ICC IP WB Rabbit IgG Log in to see 0
15.755764 ABIN5596818 ELISA IP WB Rabbit IgG C-Term Log in to see Polyclonal 0
15.755764 ABIN933020 IP WB Mouse IgG1 Log in to see E32 0
15.755764 ABIN933019 IP WB Mouse IgG1 Log in to see E19 0
14.5 ABIN967952 IF IP IHC WB Mouse IgG1 AA 325-345 Log in to see MK1 4
12.755764 ABIN6135667 IF IP WB Rabbit Log in to see 0
12.755764 ABIN1690564 IP Sepharose Rabbit Log in to see Polyclonal 0
12.755764 ABIN1690217 IP Magnetic Particles Rabbit Log in to see Polyclonal 0
11.5 ABIN967398 IP WB Mouse IgG2b C-Term Log in to see G262-118 2
8.5 ABIN559693 IP WB Mouse IgG1 Log in to see E32 2
7 ABIN5773982 ELISA ICC IP WB Rabbit IgG pT-E-pY Motif Log in to see G15-B 0
7 ABIN5773955 ELISA ICC IP WB Rabbit IgG C-Term Log in to see B20-U 0
4 ABIN126831 EIA IHC (fro) IF IP WB Mouse IgG1 pThr-Glu-pTyr Log in to see 12D4 0
4 ABIN5583090 ELISA IP WB Rabbit Log in to see Polyclonal 0
4 ABIN5583068 IP WB Biotin Mouse IgG2a Log in to see SB46b 0
4 ABIN5583069 IP WB HRP Mouse IgG2a Log in to see SB46b 0

General

Antigen Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
Epitope AA 279-379
(65), (55), (39), (38), (24), (18), (16), (16), (15), (14), (13), (11), (11), (10), (8), (7), (5), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(521), (295), (269), (51), (42), (38), (38), (24), (21), (21), (20), (10), (10), (9), (9), (8), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(401), (124), (20), (6), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAPK3 antibody is un-conjugated
(27), (25), (23), (14), (13), (13), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(448), (209), (201), (104), (85), (70), (58), (52), (39), (35), (15), (13), (8), (4), (3), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-MAPK3 Antibody

Target Details MAPK3 Application Details Handling References for anti-MAPK3 antibody (ABIN393694) Images
Specificity MAPK3 (AAH13992, 279 a.a. ~ 379 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross-Reactivity (Details) Human: ~~ Positive
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen

Antigen Type: Recombinant Protein

Target/Specificity: MAPK3 (AAH13992, 279 a.a. ~ 379 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone 3C9
Isotype IgG2a kappa
Plasmids, Primers & others

Target Details MAPK3

Product Details anti-MAPK3 Antibody Application Details Handling References for anti-MAPK3 antibody (ABIN393694) Images back to top
Antigen
Alternative Name MAPK3 (MAPK3 Antibody Abstract)
Background

Other Names: Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase, p44-ERK1, MAPK3, ERK1, PRKM3

Gene Description: mitogen-activated protein kinase 3

Gene Summary: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq

Molecular Weight 43136 DA
Gene ID 5595
NCBI Accession NP_001035145, NP_001103361, NP_002737
UniProt P27361
Pathways MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals

Application Details

Product Details anti-MAPK3 Antibody Target Details MAPK3 Handling References for anti-MAPK3 antibody (ABIN393694) Images back to top
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.

Restrictions For Research Use only

Handling

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details References for anti-MAPK3 antibody (ABIN393694) Images back to top
Buffer Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-MAPK3 antibody (ABIN393694)

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling Images back to top
Background publications

Lujan, Hakim, Moyano, Nadal, Caballero, Diaz, Valera, Carrera, Cardesa, Alos: "Activation of the EGFR/ERK pathway in high-grade mucoepidermoid carcinomas of the salivary glands." in: British journal of cancer, Vol. 103, Issue 4, pp. 510-6, 2010

Bergelin, Löf, Balthasar, Kalhori, Törnquist: "S1P1 and VEGFR-2 form a signaling complex with extracellularly regulated kinase 1/2 and protein kinase C-alpha regulating ML-1 thyroid carcinoma cell migration." in: Endocrinology, Vol. 151, Issue 7, pp. 2994-3005, 2010

Chai, Luo, Weerasinghe, Brown: "Sorafenib downregulates ERK/Akt and STAT3 survival pathways and induces apoptosis in a human neuroblastoma cell line." in: International journal of clinical and experimental pathology, Vol. 3, Issue 4, pp. 408-15, 2010

Shan, Redhu, Saleh, Halayko, Chakir, Gounni: "Thymic stromal lymphopoietin receptor-mediated IL-6 and CC/CXC chemokines expression in human airway smooth muscle cells: role of MAPKs (ERK1/2, p38, and JNK) and STAT3 pathways." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 12, pp. 7134-43, 2010

Litherland, Elias, Hui, Macdonald, Catterall, Barter, Farren, Jefferson, Rowan: "Protein kinase C isoforms zeta and iota mediate collagenase expression and cartilage destruction via STAT3- and ERK-dependent c-fos induction." in: The Journal of biological chemistry, Vol. 285, Issue 29, pp. 22414-25, 2010

Images

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling References for anti-MAPK3 antibody (ABIN393694) back to top
Supplier Images
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 279-379) antibody (ABIN393694) Immunofluorescence of monoclonal antibody to MAPK3 on HeLa cell (antibody concentrati...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 279-379) antibody (ABIN393694) anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 279-379) antibody (Image 2)
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 279-379) antibody (ABIN393694) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 279-379) antibody (ABIN393694) MAPK3 monoclonal antibody (M01), clone 3C9 Western Blot analysis of MAPK3 expression ...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 279-379) antibody (ABIN393694) Immunoperoxidase of monoclonal antibody to MAPK3 on formalin-fixed paraffin-embedded ...
Immunofluorescence (IF) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 279-379) antibody (ABIN393694) Immunofluorescence of monoclonal antibody to MAPK3 on HeLa cell. [antibody concentrat...
Immunoprecipitation (IP) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 279-379) antibody (ABIN393694) Immunoprecipitation of MAPK3 transfected lysate using anti-MAPK3 monoclonal antibody ...
ELISA image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 279-379) antibody (ABIN393694) Detection limit for recombinant GST tagged MAPK3 is approximately 0.1ng/ml as a captu...