anti-Rat (Rattus) MAPK3 antibody for Immunohistochemistry (Frozen Sections)

Recommended MAPK3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
  • ERK-1
  • ERK1
  • ERT2
  • HS44KDAP
  • HUMKER1A
  • P44ERK1
  • P44MAPK
  • PRKM3
  • p44-ERK1
  • p44-MAPK
  • Erk-1
  • Erk1
  • Ert2
  • Esrk1
  • Mnk1
  • Mtap2k
  • Prkm3
  • p44
  • p44erk1
  • p44mapk
  • fi06b09
  • wu:fi06b09
  • zERK1
  • Tb08.10J17.940
  • MAPK1
  • MNK1
  • ATMAPK3
  • ATMPK3
  • T6D9.4
  • mitogen-activated protein kinase 3
  • mitogen-activated protein kinase 3
  • mitogen activated protein kinase 3
  • mitogen-activated serine/threonine-protein kinase
  • MAPK3
  • Mapk3
  • mapk3
  • Tc00.1047053509475.10
  • Tb927.8.3550
  • CEK1
  • MPK3
Epitope
pThr-Glu-pTyr
65
55
39
38
24
18
16
16
15
14
14
11
11
10
8
7
5
5
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
521
294
268
51
42
38
38
24
21
20
20
10
10
9
9
8
7
4
3
2
2
2
1
1
1
1
Host
Mouse
401
124
20
6
1
Clonality
Monoclonal
Conjugate
This MAPK3 antibody is un-conjugated
27
25
23
14
13
13
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
448
209
202
104
85
70
58
52
39
35
15
13
7
3
3
2
1
1
1
Supplier
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Catalog No. ABIN126831
$ 555.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5551547 IF IHC (fro) WB Rabbit IgG Tyr204, pThr202 Log in to see Polyclonal 0
1 ABIN5551325 IF IHC (fro) IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN126829 EIA IHC (fro) IF IP WB Biotin Mouse IgG1 pThr-Glu-pTyr Log in to see 12D4 0

General

Antigen Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
Epitope pThr-Glu-pTyr
(65), (55), (39), (38), (24), (18), (16), (16), (15), (14), (14), (11), (11), (10), (8), (7), (5), (5), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(521), (294), (268), (51), (42), (38), (38), (24), (21), (20), (20), (10), (10), (9), (9), (8), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(401), (124), (20), (6), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAPK3 antibody is un-conjugated
(27), (25), (23), (14), (13), (13), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(448), (209), (202), (104), (85), (70), (58), (52), (39), (35), (15), (13), (7), (3), (3), (2), (1), (1), (1)
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Product Details anti-MAPK3 Antibody

Target Details MAPK3 Application Details Handling Images
Specificity This antibody specifically interacts with the pThr - Glu - pTyr motif of activated MAP kinases 1 and 2 (erk1/2). The antibody requires phosphorylation both at the threonine and the tyrosine site and does not interact with the non-phosphorylated form of the protein. Mab MAPK-12D4 shows no crossreaction with activated SAP kinases 1 or 2.
Characteristics Synonyms: ERK-1/ERK-2, MAPK1/MAPK2, P42/P44-MAPK, Extracellular signal regulated kinase 1/2,Insulin stimulated MAP2 kinase, Microtubule associated protein 2 kinase, Mitogenactivated protein kinase 1/2/3
Purification Size exclusion chromatography
Components incl. positive Control
Immunogen Synthetic phosphopeptide conjugated to KLH
Clone 12D4
Isotype IgG1
Plasmids, Primers & others

Target Details MAPK3

Product Details anti-MAPK3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAPK3 / ERK1 (MAPK3 Antibody Abstract)
Background Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.Synonyms: ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, PRKM3, p44-ERK1, p44-MAPK
Gene ID 5595
Pathways MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals

Application Details

Product Details anti-MAPK3 Antibody Target Details MAPK3 Handling Images back to top
Application Notes Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from pervanadate-treated HepG2 cells. ELISA: 0.05 μg/mL. Immunoprecipitation: 1 - 10 g per 10^6 pervanadate-treated A431 or HepG2 cells. Immunocytochemistry: 1 - 10 μg/mL. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C/-80 °C
Storage Comment Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (pThr-Glu-pTyr) antibody (ABIN126831) anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (pThr-Glu-pTyr) antibody