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|Antigen||Mitogen-Activated Protein Kinase 6 (MAPK6) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This MAPK6 antibody is un-conjugated Alternatives|
ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-MAPK6 AntibodyTarget Details MAPK6 Application Details Handling Images
|Purpose||ERK3 / MAPK6|
|Blocking Peptide||Blocking peptide for this product available: ABIN2815635|
|Specificity||Reported variants represent identical protein (NP_056621.4, NP_081694.1).|
|Predicted Reactivity||Human, Mouse, Rat, Dog, Pig, Cow|
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Plasmids, Primers & others|
Target Details MAPK6Product Details anti-MAPK6 Antibody Application Details Handling Images back to top
|Alternative Name||Mapk6 (MAPK6 Antibody Abstract)|
|Background||MAPK6, mitogen-activated protein kinase 6, ERK3, HsT17250, PRKM6, p97MAPK, ERK-3, MAP kinase 6, MAP kinase isoform p97, MAPK 6, extracellular signal-regulated kinase 3, extracellular signal-regulated kinase, p97, p97-MAPK, protein kinase, mitogen-activated 5, protein kinase, mitogen-activated 6|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C|
Application DetailsProduct Details anti-MAPK6 Antibody Target Details MAPK6 Handling Images back to top
DS WB Results: In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and the same band is observed using anti-GFP antibody. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1 μg/mL.
DS IHC Results: Paraffin embedded Human Heart. Recommended concentration: 10 μg/mL.
Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:128000.
Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug EB11926. Western blots of the IP were labelled with rabbit anti-ERK3 serum (CST) or with mouse anti-MK5 (SC). An approx 100kDa of ERK3 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in as described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPK6 Antibody Target Details MAPK6 Application Details Images back to top
|Buffer||Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Minimize freezing and thawing.|
|Storage Comment||Aliquot and store at -20 °C.|
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