anti-Human MAPK6 antibody for Immunoprecipitation

Recommended MAPK6 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 6 (MAPK6) Antibodies
  • ERK3
  • HsT17250
  • PRKM6
  • p97MAPK
  • 2610021I23Rik
  • D130053K17Rik
  • Erk3
  • Mapk4
  • Mapk63
  • Prkm4
  • Prkm6
  • ATMAPK6
  • ATMPK6
  • F18O19.10
  • MAP KINASE 6
  • MAP kinase 6
  • MAPK6
  • SUSERK3
  • erk3
  • p97mapk
  • si:ch211-235f12.1
  • mitogen-activated protein kinase 6
  • MAP kinase 6
  • MAPK6
  • Mapk6
  • MPK6
  • mapk6
Epitope
Internal Region
29
12
8
6
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
152
97
78
10
7
6
6
5
5
4
3
2
2
1
1
1
Host
Goat
116
26
15
5
Clonality
Polyclonal
Conjugate
This MAPK6 antibody is un-conjugated
7
6
5
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
119
97
49
26
17
13
12
10
7
5
2
2
1
Options
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Catalog No. ABIN1590009
$ 429.84
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.643548 ABIN519219 IP Rabbit AA 1-721 Log in to see Polyclonal 0
1 ABIN1911982 IP WB Rabbit IgG AA 671-721 Log in to see Polyclonal 0
1 ABIN4951671 ELISA IP WB Goat Ig Fraction Log in to see Polyclonal 0
1 ABIN2562470 IP ELISA WB Goat IgG Internal Region Log in to see Polyclonal 0
1 ABIN5541249 EIA IP WB Goat C-Term, Internal Region Log in to see Polyclonal 0
1 ABIN2306407 IP Rabbit Log in to see Polyclonal 0
1 ABIN5558310 ELISA IP WB Biotin Chicken Log in to see Polyclonal 0
1 ABIN5558311 ELISA IP WB FITC Chicken Log in to see Polyclonal 0
1 ABIN5558309 ELISA IP WB Chicken Log in to see Polyclonal 0

General

Antigen Mitogen-Activated Protein Kinase 6 (MAPK6) Antibodies
Epitope Internal Region
(29), (12), (8), (6), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(152), (97), (78), (10), (7), (6), (6), (5), (5), (4), (3), (2), (2), (1), (1), (1)
Host Goat
(116), (26), (15), (5)
Clonality
Polyclonal
Conjugate This MAPK6 antibody is un-conjugated
(7), (6), (5), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(119), (97), (49), (26), (17), (13), (12), (10), (7), (5), (2), (2), (1)
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Product Details anti-MAPK6 Antibody

Target Details MAPK6 Application Details Handling Images
Purpose ERK3 / MAPK6
Blocking Peptide Blocking peptide for this product available: ABIN2815635
Sequence HSPVGSPLKS IQ
Specificity Reported variants represent identical protein (NP_056621.4, NP_081694.1).
Predicted Reactivity Human, Mouse, Rat, Dog, Pig, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Plasmids, Primers & others

Target Details MAPK6

Product Details anti-MAPK6 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Mapk6 (MAPK6 Antibody Abstract)
Background MAPK6, mitogen-activated protein kinase 6, ERK3, HsT17250, PRKM6, p97MAPK, ERK-3, MAP kinase 6, MAP kinase isoform p97, MAPK 6, extracellular signal-regulated kinase 3, extracellular signal-regulated kinase, p97, p97-MAPK, protein kinase, mitogen-activated 5, protein kinase, mitogen-activated 6
Gene ID 5597
NCBI Accession NP_002739
Pathways MAPK Signaling, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C

Application Details

Product Details anti-MAPK6 Antibody Target Details MAPK6 Handling Images back to top
Application Notes

DS WB Results: In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and the same band is observed using anti-GFP antibody. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1 μg/mL.

DS IHC Results: Paraffin embedded Human Heart. Recommended concentration: 10 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:128000.

Comment

Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug EB11926. Western blots of the IP were labelled with rabbit anti-ERK3 serum (CST) or with mouse anti-MK5 (SC). An approx 100kDa of ERK3 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in as described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.

Restrictions For Research Use only

Handling

Product Details anti-MAPK6 Antibody Target Details MAPK6 Application Details Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

Images

Product Details anti-MAPK6 Antibody Target Details MAPK6 Application Details Handling back to top
Supplier Images
 image for anti-Mitogen-Activated Protein Kinase 6 (MAPK6) (Internal Region) antibody (ABIN1590009) ABIN1590009 (1.5ug) immunoprecipitation from lysates of MK5/ERK3 double knockout MEFs...
 image for anti-Mitogen-Activated Protein Kinase 6 (MAPK6) (Internal Region) antibody (ABIN1590009) HEK293 lysate (10 µg protein in RIPA buffer) overexpressing GFP-fused Mouse ERK3 prob...