anti-Mouse (Murine) MAPK6 antibody for Enzyme Immunoassay

Recommended MAPK6 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 6 (MAPK6) Antibodies
  • ERK3
  • HsT17250
  • PRKM6
  • p97MAPK
  • 2610021I23Rik
  • D130053K17Rik
  • Erk3
  • Mapk4
  • Mapk63
  • Prkm4
  • Prkm6
  • ATMAPK6
  • ATMPK6
  • F18O19.10
  • MAP KINASE 6
  • MAP kinase 6
  • MAPK6
  • SUSERK3
  • erk3
  • p97mapk
  • si:ch211-235f12.1
  • mitogen-activated protein kinase 6
  • MAP kinase 6
  • MAPK6
  • Mapk6
  • MPK6
  • mapk6
Epitope
C-Term, Internal Region
28
11
8
7
6
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
151
97
77
9
6
6
5
5
5
4
3
2
2
1
1
1
Host
Goat
115
27
15
5
Clonality
Polyclonal
Conjugate
This MAPK6 antibody is un-conjugated
7
6
5
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
119
98
49
27
17
13
12
10
8
5
2
1
1
Supplier
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Catalog No. ABIN5541249
$ 536.67
Plus shipping costs $45.00

General

Antigen Mitogen-Activated Protein Kinase 6 (MAPK6) Antibodies
Epitope C-Term, Internal Region
(28), (11), (8), (7), (6), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(151), (97), (77), (9), (6), (6), (5), (5), (5), (4), (3), (2), (2), (1), (1), (1)
Host Goat
(115), (27), (15), (5)
Clonality
Polyclonal
Conjugate This MAPK6 antibody is un-conjugated
(7), (6), (5), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(119), (98), (49), (27), (17), (13), (12), (10), (8), (5), (2), (1), (1)
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Product Details anti-MAPK6 Antibody

Target Details MAPK6 Application Details Handling Images
Sequence C-HSPVGSPLKS IQ
Specificity Recognizes MAPK6 / ERK3.
Purification Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Immunogen Peptide with sequence from the internal region (near C-Terminus) of the protein sequence according to NP_002739.1.
Plasmids, Primers & others

Target Details MAPK6

Product Details anti-MAPK6 Antibody Application Details Handling Images back to top
Antigen
Alternative Name mapk6,erk3 (MAPK6 Antibody Abstract)
Pathways MAPK Signaling, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C

Application Details

Product Details anti-MAPK6 Antibody Target Details MAPK6 Handling Images back to top
Application Notes Peptide ELISA: 1/128000 (Detection Limit). Western blot: 0.5-1 μg/mL. In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and the same band is observed using anti-GFP antibody. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 μg MAPK6 / ERK3 Antibody Cat.-No AP32807PU-N. Western blots of the IP were labelled with Rabbit anti-ERK3 serum (CST) or with Mouse anti-MK5 (SC). An approx 100 kDa of ERK3 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.
Restrictions For Research Use only

Handling

Product Details anti-MAPK6 Antibody Target Details MAPK6 Application Details Images back to top
Format Liquid
Buffer Tris saline, pH ~7.3 Preservatives: 0.02 % Sodium Azide Stabilizers: 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.