anti-Monkey MAPKAP Kinase 2 antibody for ELISA

Recommended MAPKAP Kinase 2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) Antibodies
  • mapkapk2
  • wu:fb30e10
  • wu:fc56c10
  • wu:fi03a01
  • MGC78852
  • MAPKAPK2
  • AA960234
  • MAPKAP-K2
  • MK-2
  • MK2
  • Rps6kc1
  • mitogen-activated protein kinase-activated protein kinase 2a
  • mitogen-activated protein kinase-activated protein kinase 2 L homeolog
  • mitogen-activated protein kinase-activated protein kinase 2
  • MAP kinase-activated protein kinase 2
  • mapkapk2a
  • mapkapk2.L
  • MAPKAPK2
  • Mapkapk2
Epitope
AA 44-55
55
31
22
15
15
15
15
9
9
7
6
5
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey, Mouse (Murine)
229
172
135
44
14
9
6
6
6
5
4
4
3
2
2
1
Host
Goat
202
25
25
Clonality
Polyclonal
Conjugate
This MAPKAP Kinase 2 antibody is un-conjugated
8
8
8
6
5
5
4
4
3
3
3
3
3
3
3
3
3
Application
ELISA, Western Blotting (WB)
192
104
83
46
39
37
27
17
12
4
4
3
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Catalog No. ABIN1833999
$ 610.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3185455 ELISA IHC WB Rabbit IgG Tyr199, Tyr458 Log in to see Polyclonal 0
1 ABIN3182577 ELISA IHC WB Rabbit IgG pThr222 Log in to see Polyclonal 0

General

Antigen Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) Antibodies
Epitope AA 44-55
(55), (31), (22), (15), (15), (15), (15), (9), (9), (7), (6), (5), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey, Mouse (Murine)
(229), (172), (135), (44), (14), (9), (6), (6), (6), (5), (4), (4), (3), (2), (2), (1)
Host Goat
(202), (25), (25)
Clonality
Polyclonal
Conjugate This MAPKAP Kinase 2 antibody is un-conjugated
(8), (8), (8), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application ELISA, Western Blotting (WB)
(192), (104), (83), (46), (39), (37), (27), (17), (12), (4), (4), (3), (1), (1), (1)
Supplier Log in to see

Product Details anti-MAPKAP Kinase 2 Antibody

Target Details MAPKAP Kinase 2 Application Details Handling Images
Specificity Human MAPKAPK2 / MK2. This antibody is expected to recognize both reported isoforms (NP_004750.1, NP_116584.2).
Purification Immunoaffinity purified
Immunogen Synthetic peptide C-PQFHVKSGLQIK from an internal region of human MAPKAPK2 / MK2 (NP_004750.1, NP_116584.2). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey (100%), Galago, Mouse, Rat, Panda, Rabbit, Pig, Guinea pig (92%), Bovine, Opossum (83%).

Type of Immunogen: Synthetic peptide

Target Details MAPKAP Kinase 2

Product Details anti-MAPKAP Kinase 2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAPKAPK2 / MK2 (MAPKAPK2 Antibody Abstract)
Background Name/Gene ID: MAPKAPK2
Subfamily: MAPKAPK
Family: Protein Kinase

Synonyms: MAPKAPK2, MK-2, MAP KAP Kinase 2, MAPKAP kinase 2, MK2, MAPKAP-K2, MAPKAPK-2
Gene ID 9261
NCBI Accession NP_004750, NP_116584
UniProt P49137
Pathways MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades

Application Details

Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Handling Images back to top
Application Notes Approved: ELISA (1:32000), WB (0.5 - 2 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: In transfected HEK293 transiently expressing either Mouse MK2 fused to GFP or the similar Mouse MK5 fused to GFP, only the expected band was observed in the MK2 lane after EB11929 and EB11930 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration: 0.5-2 μg/mL. Immunoprecipitation: MK2/MK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK2 or empty vector were lysed from confluent plates and used for IP with 1.5 μg EB11929 or EB11930. Western blots of the IP were labeled with rabbit anti-MK2 (CST). An approx 45kD of MK2 is only precipitated from lysates of those KO MEFs that have been rescued by the MK2 expression construct as described in PMID: 19743408. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.
Restrictions For Research Use only

Handling

Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store at -20°C. Minimize freezing and thawing.