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|Antigen||Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies|
|Epitope||C-Term, Isoform 1 Alternatives|
|Reactivity||Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MCL-1 antibody is un-conjugated Alternatives|
Western Blotting (WB)
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Product Details anti-MCL-1 AntibodyTarget Details MCL-1 Application Details Handling Images
|Specificity||This antibody is expected to recognise isoform 1 (NP_068779.1).|
Species reactivity (expected):Mouse, Rat, Canine (Dog) and Feline (Cat).
Species reactivity (tested):Human.
|Purification||Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.|
|Immunogen||Peptide from the C Terminus of the protein sequence according to NP_068779.1|
Target Details MCL-1Product Details anti-MCL-1 Antibody Application Details Handling Images back to top
|Alternative Name||MCL1 (MCL1 Antibody Abstract)|
|Background||Mcl1 is an anti-apoptotic member of Bcl2 family originally isolated from the ML1 human myeloid leukemia cell line during phorbol ester-induced differentiation along the monocyte/macrophage pathway. Mcl1 localizes to the mitochondria, interacts with and antagonizes pro-apoptotic Bcl2 family members, and inhibits apoptosis by a number of cytotoxic stimuli. It is involved in programing of differentiation and concomitant maintenance of viability but not of proliferation. Isoform 1 inhibits apoptosis while isoform 2 promotes it. Expression increases early during phorbol-ester induced differentiation along the monocyte/macrophage pathway in myeloid leukemia cell lines ML1.Synonyms: BCL2L3, Bcl-2-like protein 3, Bcl-2-related protein, EAT, Induced myeloid leukemia cell differentiation protein Mcl-1, mcl1/EAT, r004|
|Research Area||Apoptosis/Necrosis, Cancer|
Application DetailsProduct Details anti-MCL-1 Antibody Target Details MCL-1 Handling Images back to top
Peptide ELISA: 1/2,000. Western Blot: 1-3 μg/mL. Approx 35 kDa band observed in lysates of Human Lung and cellline K562 (calculated MW of 37.5 kDa according to NP_068779.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Images back to top
|Buffer||Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|