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|Antigen||Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies|
|Epitope||AA 121-139 Alternatives|
|Conjugate||This MCL-1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
|6 references available|
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Product Details anti-MCL-1 AntibodyTarget Details MCL-1 Application Details Handling References for anti-MCL-1 antibody (ABIN967399) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. This product contains thimerosal, an organic mercury compound. Mercury and mercury compounds are chemicals known to the State of California to cause birth defects or other reproductive harm. Foreseeable use of this product does not pose a known reproductive toxicity threat.
4. Please refer to us for technical protocols.
|Immunogen||Human Mcl-1 aa. 121-139 Peptide|
Target Details MCL-1Product Details anti-MCL-1 Antibody Application Details Handling References for anti-MCL-1 antibody (ABIN967399) Images back to top
|Alternative Name||Mcl-1 (MCL1 Antibody Abstract)|
|Background||Mcl-1 (for myeloid cell leukemia-1) is a member of the Bcl-2 family that was identified by screening of of cDNA libraries derived from differentiating human myeloid leukemia cells. Bcl-2 family members are involved in apoptosis and share two highly conserved functional regions, Bcl-2 homology 1 and 2 (BH1 and BH2). Several of the family members including Bcl-2 act as inhibitors of apoptosis, whereas others such as Bax promote cell death. It is thought that protein-protein interactions between Bcl-2 family members play an important role in their function. For example, Bax can homodimerize as well as heterodimerize with Bcl-2. When Bax is present in excess, it can counteract the ability of Bcl-2 to inhibit cell death. Although its function remains to be fully elucidated, Mcl-1 may, like Bcl-2, be a blocker of cell death. For example, transfection of Mcl-1 into Chinese hamster ovary cells partially blocked Myc-induced apoptosis, and Mcl-1 blocked Bax mediated cell death in a yeast two-hybrid system. The two-hybrid system results suggest that Mcl-1/Bax interations may have functional homology to Bcl-2/Bax interations in blocking Bax-mediated cell death. This polyclonal antibody is not recommended for the identification of mouse Mcl-1 and has been reported not to cross-react with human Bcl-2.|
|Molecular Weight||39-42 kDa|
Application DetailsProduct Details anti-MCL-1 Antibody Target Details MCL-1 Handling References for anti-MCL-1 antibody (ABIN967399) Images back to top
Related Products: ABIN967390
|Restrictions||For Research Use only|
HandlingProduct Details anti-MCL-1 Antibody Target Details MCL-1 Application Details References for anti-MCL-1 antibody (ABIN967399) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide and ≤0.03 % thimerosal|
|Preservative||Sodium azide, Thimerosal (Merthiolate)|
|Precaution of Use||This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4°C.|
References for anti-MCL-1 antibody (ABIN967399)Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Handling Images back to top
|Product cited in:||
Ohta, Iwai, Kasahara, Taniguchi, Krajewski, Reed, Miyawaki: "Immunoblot analysis of cellular expression of Bcl-2 family proteins, Bcl-2, Bax, Bcl-X and Mcl-1, in human peripheral blood and lymphoid tissues." in: International immunology, Vol. 7, Issue 11, pp. 1817-25, 1996
Reynolds, Yang, Qian, Jenkinson, Zhou, Eastman, Craig: "Mcl-1, a member of the Bcl-2 family, delays apoptosis induced by c-Myc overexpression in Chinese hamster ovary cells." in: Cancer research, Vol. 54, Issue 24, pp. 6348-52, 1995
Krajewski, Bodrug, Gascoyne, Berean, Krajewska, Reed: "Immunohistochemical analysis of Mcl-1 and Bcl-2 proteins in normal and neoplastic lymph nodes." in: The American journal of pathology, Vol. 145, Issue 3, pp. 515-25, 1994
Sato, Hanada, Bodrug, Irie, Iwama, Boise, Thompson, Golemis, Fong, Wang: "Interactions among members of the Bcl-2 protein family analyzed with a yeast two-hybrid system." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 20, pp. 9238-42, 1994
Kozopas, Yang, Buchan, Zhou, Craig: "MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3516-20, 1993
Krajewski, Bodrug, Krajewska, Shabaik, Gascoyne, Berean, Reed et al.: "Immunohistochemical analysis of Mcl-1 protein in human tissues. Differential regulation of Mcl-1 and Bcl-2 protein production suggests a unique role for Mcl-1 in control of programmed cell death in ..." in: The American journal of pathology, Vol. 146, Issue 6, pp. 1309-19, 1995
ImagesProduct Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Handling References for anti-MCL-1 antibody (ABIN967399) back to top