anti-Human PAK1 antibody for Immunocytochemistry

Recommended PAK1 Antibody (supplied by: Log in to see )

Antigen
P21-Activated Kinase 1 (PAK1) Antibodies
  • X-PAK1
  • XPak1
  • pak
  • pak-1
  • pakalpha
  • PAKalpha
  • AW045634
  • PAK-1
  • Paka
  • p21 protein (Cdc42/Rac)-activated kinase 1 S homeolog
  • p21 (RAC1) activated kinase 1
  • p21 protein (Cdc42/Rac)-activated kinase 1
  • Serine/threonine-protein kinase pak-1
  • pak1.S
  • PAK1
  • Pak1
  • pak-1
Epitope
Center
63
33
21
15
12
12
11
11
9
8
6
5
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
340
181
172
9
9
6
5
4
4
3
2
1
1
Host
Rabbit
306
17
16
1
Clonality
Polyclonal
Conjugate
This PAK1 antibody is un-conjugated
11
11
11
7
7
7
4
4
4
4
4
4
4
4
4
4
4
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
247
127
116
73
55
52
22
20
10
7
6
5
4
1
Options
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Catalog No. ABIN2855705
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4343458 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN1874025 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN4343459 ICC IF IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
1 ABIN2743439 ICC IF IHC (p) WB Rabbit Internal Region Log in to see Polyclonal 0
1 ABIN2743440 ICC IF IHC (p) WB Rabbit pThr212 Log in to see Polyclonal 0
1 ABIN2880325 ICC IHC IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN4343457 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal 0
1 ABIN1982259 ICC Sheep IgG AA 128-242 Log in to see Polyclonal 0
1 ABIN3023361 ICC IP IHC WB Rabbit Log in to see Polyclonal 0
1 ABIN2859027 ICC IF IHC WB Rabbit Log in to see Polyclonal 0

General

Antigen P21-Activated Kinase 1 (PAK1) Antibodies
Epitope Center
(63), (33), (21), (15), (12), (12), (11), (11), (9), (8), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(340), (181), (172), (9), (9), (6), (5), (4), (4), (3), (2), (1), (1)
Host Rabbit
(306), (17), (16), (1)
Clonality
Polyclonal
Conjugate This PAK1 antibody is un-conjugated
(11), (11), (11), (7), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(247), (127), (116), (73), (55), (52), (22), (20), (10), (7), (6), (5), (4), (1)
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Product Details anti-PAK1 Antibody

Target Details PAK1 Application Details Handling Images
Cross-Reactivity Cow (Bovine)
Cross-Reactivity (Details) Bovine (100 %)
Characteristics Rabbit Polyclonal antibody to PAK1 (p21 protein (Cdc42/Rac)-activated kinase 1)
PAK1 antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human PAK1. The exact sequence is proprietary.
Isotype IgG

Target Details PAK1

Product Details anti-PAK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PAK1 (PAK1 Antibody Abstract)
Background PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Cytoplasm , Cell junction , focal adhesion
Molecular Weight 61 kDa
Gene ID 5058
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis

Application Details

Product Details anti-PAK1 Antibody Target Details PAK1 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:10000*
Comment

Positive Control: A431 , HeLa , BCL-1 , PC-12 , SH-SY5Y

Restrictions For Research Use only

Handling

Product Details anti-PAK1 Antibody Target Details PAK1 Application Details Images back to top
Format Liquid
Concentration 0.48 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-PAK1 Antibody Target Details PAK1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-P21-Activated Kinase 1 (PAK1) (Center) antibody (ABIN2855705) WB Image PAK1 antibody detects PAK1 protein by Western blot analysis. A. 30 µg BCL-1 ...
Western Blotting (WB) image for anti-P21-Activated Kinase 1 (PAK1) (Center) antibody (ABIN2855705) WB Image PAK1 antibody detects PAK1 protein by Western blot analysis. A. 30 µg PC-12 ...
Immunofluorescence (IF) image for anti-P21-Activated Kinase 1 (PAK1) (Center) antibody (ABIN2855705) ICC/IF Image PAK1 antibody detects PAK1 protein at cytoplasm by immunofluorescent ana...
Immunohistochemistry (IHC) image for anti-P21-Activated Kinase 1 (PAK1) (Center) antibody (ABIN2855705) IHC-P Image PAK1 antibody detects PAK1 protein on human hepatoma by immunohistochemic...
Western Blotting (WB) image for anti-P21-Activated Kinase 1 (PAK1) (Center) antibody (ABIN2855705) WB Image PAK1 antibody detects PAK1 protein by western blot analysis. Whole cell extr...
Western Blotting (WB) image for anti-P21-Activated Kinase 1 (PAK1) (Center) antibody (ABIN2855705) WB Image Sample (30 ug of whole cell lysate) A: A431 B: HeLa 7.5% SDS PAGE antibody d...