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|Antigen||P21-Activated Kinase 2 (PAK2) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PAK2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-PAK2 AntibodyTarget Details PAK2 Application Details Handling Images
|Specificity||This antibody detects PAK2 at N-term.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, mouse, rat|
|Purification||Peptide affinity chromatography|
|Immunogen||14 amino acid peptide from near the amino terminus of human PAK2|
Target Details PAK2Product Details anti-PAK2 Antibody Application Details Handling Images back to top
|Alternative Name||PAK2 (PAK2 Antibody Abstract)|
|Background||The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6, and are not activated by Cdc42/Rac binding. Of the six PAK proteins, only PAK2 is ubiquitously expressed and cleaved by caspase-3. This cleavage removes the amino-terminal regulatory domain and generates a constitutively active kinase fragment. Recent experiments have shown that following cleavage, the active fragment is myristoylated and directed to the plasma membrane and membrane ruffles where it promotes cell death via increased signaling through the c-Jun N-terminal kinase pathway, but without compromising mitochondrial integrity.Synonyms: Gamma-PAK, PAK 2, PAK gamma, PAK-2, PAK65, Serine/threonine-protein kinase PAK 2, p21-activated kinase 2, p58|
|Research Area||Apoptosis/Necrosis, Cancer, Kinases/Phosphatases, Tyrosine Kinases|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha|
Application DetailsProduct Details anti-PAK2 Antibody Target Details PAK2 Handling Images back to top
ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PAK2 Antibody Target Details PAK2 Application Details Images back to top
|Buffer||PBS containing 0.02 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.|